Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Genetic Relatedness within Streptococcus pneumoniae Serotype 19F and 23F Isolates in Korea by Pulsed-Field Gel Electrophoresis

  • Lee, Kwang-Jun (Laboratory of Respiratory Infections, Department of Bacteriology, National Institute of Heath) ;
  • Bae, Song-Mee (Laboratory of Respiratory Infections, Department of Bacteriology, National Institute of Heath) ;
  • Hwang, Kyu-Jam (Laboratory of Respiratory Infections, Department of Bacteriology, National Institute of Heath) ;
  • Lee, Young-Hee (Laboratory of Respiratory Infections, Department of Bacteriology, National Institute of Heath) ;
  • Kim, Ki-Sang (Laboratory of Respiratory Infections, Department of Bacteriology, National Institute of Heath)
  • Published : 2003.03.01
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Abstract

The genetic relatedness of multidrug-resistant pneumococcal isolates of serotypes 19F and 23F was investigated. The DNA fragments digested with Sma I were resolved by pulsed-field gel electrophoresis (PFGE). PFGE analysis of 365. pneumoniae isolates showed 13 different patterns. Among 22 isolates of serotype 19F, 9 different PFGE patterns were present and 14 isolates of serotype 23F isolates represented 5 distinct PFGE patterns. Two isolates of serotype 19F and six isolates of serotype 23F shared the same PFGE pattern (Pattern I). Based on the genetic relatedness within the strains (one genetic cluster was defined as having more than 85% homology), we divided the pneumococcal strains into genefic clusters (Ⅰ, II, III, IV, V, and VI). The 22 strains of serotype 19F belonged to five distinct genetic clusters (I, II, III, IV, V and VI) and 14 strains of serotype 23F represented two genetic clusters (I and II ). These results showed that strains of serotype 19F are genetically more diverse than those of serotype 23F, Serotype 19F isolates with PFGE patterns H and I appeared to be less related to those of the remaining PFCE patterns (A to G) (less than 60% genetic relatedness), but those strains were genetically closely related with serotype 23f. These results suggest that the latter isolates originated from horizontal transfer of the capsular type 19F gene locus to 23F pneumococcal genotypes. In conclusion, the multidrug-resistant pneumococcal isolates of serotype 19f and 23F isolated in Korea are the result of the spread of a limited number of resistant clones.

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References

  1. Clin. Infect. Dis v.15 Antimicrobial resistance in Streptococcus pneumoniae Appelbaum, P.C. https://doi.org/10.1093/clinids/15.1.77
  2. Manual of clinical microbiology(5th ed.) Streptococcus and related catalase-negative gram-positive cocci Facklam, R.R.;J.A. Washington;A. Balows(ed.);W.J. Hausler(ed.);R.L. Hermann(ed.);H.D. Isenberg(ed.);H.J. Shsadomy (ed.)
  3. J. Clin. Microbiol. v.34 Clonal distribution of penicillin-resistant Streptococcus pneumoniae 23F in France Ferroni, A.;L. Nguyen;P.gehanno;I. Boucot;P. Berche
  4. Microb. Drug. Resist. v.3 Molecular epidemiology of drug-resistant pneumococci: toward and international approach Hermans, P.W.M.;M. Sluijter;S. Dejsirilert;N. Lemmens;K. Elzenaar;A. van Veen;W.H.F. Goessens;R. de Groot https://doi.org/10.1089/mdr.1997.3.243
  5. Semin. pediatr. Infect. Dis. v.7 The emerging antibiotic resistance of Streptococcus pneumoniae Kaplan, S. https://doi.org/10.1016/S1045-1870(96)80015-X
  6. Microb. Drug. Resist. v.2 High incidence of multidrug-resistant Streptococcus pneumoniae in South Korea Kim, S.N.;S.W. Kim;I.H. Choi;S.N. Pyo;D.K. Rhee https://doi.org/10.1089/mdr.1996.2.401
  7. J. Infect. Dis. v.164 Intercontinental spread of a multiresistant clone of serotype 23F Streptococcus pneumoniae Munoz, R.;T.R. Coffey;M. Daniels;C.G. Dowson;G. Laible;J. Casal;R. Hakenbeck;M. Jacobs;J.M. Musser;B.G. Spratt;A. Tomasz
  8. Performance standards for antimicrobial susceptibility testing, 9th informational supplement. M100-S9 National Committee for Clinical Laboratory Standards
  9. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically(4th ed.)Approved standatds M7-A4. National Committee for Clinical Laboratory Standards
  10. J. Infect. Dis v.177 Capsular trasformation of a multidrug-resistant Streptococcus pneumoniae in vivo Nesin, M.;M. Ramirez;A. Tomasz https://doi.org/10.1086/514242
  11. Clin. Infect. Dis v.21 Antimicrobial resistance and type distribution of Streptococcus pneumoniae isolates causing systemic infections in Germany Reinert, R.R.;A. Queck;A. Kaufhold;M. Kresken;R. Lutticken https://doi.org/10.1093/clinids/21.6.1398
  12. J. Med. Microbiol. v.43 Penicillin-resistant Streptococcus pneumoniae in Germany:genetic relationship to clones from other European countries Reichmann, P.;E. Varon;E. Gunther;R.R. Reinert;R. Littiken;A. Marton;P. Geslin;J. Wagner;R. Hakenbeck https://doi.org/10.1099/00222615-43-5-377
  13. J. Infect. Dis. v.148 Considerations for formulating the second-generation pneumococcal capsular polysaccharide vaccine with emphasis on the cross-reactive types within groups Robbins. J.B.;R. Austrian;C.-J. Lee;S.C. Rastogi;G. Schiffman;J. Henrichsen;P.H. Makela;C.V. Broome;R.R. Facklam;R.H.Tiesjema;J.C. Parke Jr. https://doi.org/10.1093/infdis/148.6.1136
  14. Clin Infect Dis v.28 Spread of drug-resistant Streptococcus pneumoniae in Asian countries: Asian Network for Surveillance of Resistnat Pathogens (ANSORP) Study Song J.H.;N.Y. Lee;S. Ichiyama;R. Yoshida;Y. Hirakata;W. Fu;A.Chongthaleong;N. Aswapokee;C.H. Chiu;M.K. Latitha;K. Thomas;J. Perera;T.T. Yee;F. Jamal;U.C. Warsa;B.X. Vinh;M.R. Jacobs;P.C. Appelbaum;the ANSORP Study Group https://doi.org/10.1086/514783
  15. J. Clin. Microbiol. v.33 Interpreting chromosonmal DNA restriction patterns produced by pulsedfield gel electrophoresis: Criteria for bacterial strain typing Tenover, F.C.;R.D. Arbeit;R.V. Goering;P.A. Mickelsen;B.E. Murray;D.H. Persing;B. Swaminathan
  16. N. Engl. J. Med. v.332 Pathogenesis of pneumococcal infection Tuomanen, E.I.;R. Austrian;H.R. Masure https://doi.org/10.1056/NEJM199505113321907