Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biotransformation of a Fungicide Ethaboxam by Soil Fungus Cunninghamella elegans

  • PARK, MI-KYUNG (School of Agricultural Biotechnology, Seoul national University) ;
  • KWANG-HYEON LIU (Department of Pharmacology, Inje university College of Medicine) ;
  • YOONGHO LIM (Department of Pharmacology, Inje university College of Medicine) ;
  • YOUN-HYUNG LEE (Plant Metabolism Research Center, Kyung Hee University) ;
  • HOR-GIL HUR (Department of Environmental Science & Engineering, Kwangju Institute of Science & Technology) ;
  • JEONG-HAN KIM (School of Agricultural Biotechnology, Seoul national University)
  • Published : 2003.02.01
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Abstract

Metabolism of a new fungicide ethaboxam by soil fungi was studied. Among the fungi tested, Cunninghamelia elegans produced metabolites from ethaboxam, which were not found in the control experiments. M5, a major metabolite from ethaboxam was firmly identified as N-deethylated ethaboxam by LC/MS/MS and NMR. N-Deethylated ethaboxam has been found as a single metabolite in in vitro metabolism with rat liver microsomes. Ml was proved to be 4-ethyl-2-(ethylamino)-1,3-thiazole-5-carboxamide (ETC) by comparing with the authentic compound. In addition, M2, M3, and M4, and M6 were tentatively Identified by LC/MS/MS as hydroxylated and methoxylated ethaboxams, respectively. Production of the major metabolite, N-deethylated ethaboxam, by the fungus suggested that C. elegans would be an efficient eukaryotic microbial candidate for evaluating xenobiotic-driven mammalian risk assessment.

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