Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genotoxicity Assay Using Chromosomally-Integrated Bacterial recA::Lux

  • Min, Ji-Ho (National Research Laboratory of Environmental Biotechnology, Kwangju Institute of Science and Technology(K-JIST)) ;
  • Gu, Man-Bock (National Research Laboratory of Environmental Biotechnology, Kwangju Institute of Science and Technology(K-JIST))
  • Published : 2003.02.01
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Abstract

An Escherichia coli strain containing the recA promoter that fused to the luxCDABE operon originating from Photorhabdus luminescens was shown to respond sensitively to genotoxic stresses. Two different recombinant bacteria, one (DPDI 657) harboring a plasmid with the recA promoter that fused to the luxCDABE operon, and the other (DPD1710) containing a chromosomally-integrated recA promoter that fused with luxCDABE, were compared and it was found that the sensitivity of 'the two strains was significantly different in terms of their bioluminescent level, response time, and the minimum detectable concentration of a chemical causing DNA damaging stress. DPDI 710, with a chromosomally-integrated single copy, generally led to lower basal luminescence levels, faster responses, increased response ratios, and an enhanced sensitivity to mutagens, when compared to DPD 1657 with a multi-copy plasmid.

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References

  1. Mutat. Res. v.31 Methods for detecting carciogens and mutagens with the Salmonellal mammalian microsome mutagenicity test Ames B. N.;J. McCann;E. Yamasaki https://doi.org/10.1016/0165-1161(75)90046-1
  2. J. Microbiol. Biotechnol. v.7 Stabilization of bioluminescence of immobilized Photobacterium phosphoreum and monitoring of environmental pollutants Britz M. L.;N. Simonov;U. H. Chun
  3. J. Microbiol. Biotechnol. v.7 Immobilized luminescent cell-based flow through monitoring of environmental pollutants Britz M. L.;N. Simonov;U. H. Chun
  4. Chemosphere v.46 Toxicity monitoring and classification of Endocrine-Disrupting Chemicals (EDCs) using recombinant bioluminescent bacteria Gu M. B.;J. Min;E. J. Kim https://doi.org/10.1016/S0045-6535(01)00081-9
  5. J. Biochem. Biophys. Meth. v.45 Bacterial bioluminescent emission from recombinant Escherichia coli harboring a recA::luxCDABE fusion Gu M. B.;J. Min;R. A. LaRossa https://doi.org/10.1016/S0165-022X(00)00100-7
  6. J. Microbiol. Biotechnol. v.4 Characterization of a cadmium-resistant yeast strain in response to cadmium or heat shock stress Huh N. E.;N. S. Choi;Y. K. Seo;T. S. Yu;H. S. Lee
  7. Environ. Sci. Technol. v.30 Rapid detection of mutagens by induction of luciferase-bearing prophage in Escherichia coli. Karine J.;J. M. Mailiard;J. Benedik;R. C. Wilson https://doi.org/10.1021/es950774t
  8. Biotech. Bioprocess Eng. v.5 Toxicity monitoring of endocrine disrupting chemicals (EDCs) using freeze-dried recombinant bioluminescent bacteria Kim S. W.;S. H. Choi;J. Min;M. B. Gu https://doi.org/10.1007/BF02932344
  9. J. Microbiol. Biotechnol. v.10 Stress responses of the Escherichia coli groE promoter Kwak Y. H.;J. K. Sung;Y. L. Ki;K. B. Han
  10. J. Microbiol. Biotechnol. v.11 Characterization of plasmids from Bifidobacterium sp. Lee J. H.;M. S. Park;K. H. Lee;G. E. Ji
  11. Mutat. Res. v.442 Distinct responses of a recA::luxCDABE Escherichia coli strain to direct and indirect DNA damaging agents Min J.;E. J. Kim;R. A. LaRossa;M. B. Gu https://doi.org/10.1016/S1383-5718(99)00059-5
  12. J. Microbiol. Biotechnol. v.12 Construction and characterization of a recombinant bioluminescence Streptomycetes for potential environmental monitoring Park H. J.;K. O. Hwang;E. S. Kim
  13. Appl. Env. Microbiol. v.63 A biosensor for environmental genotoxin screening based on a SOS lux assay in recombinant Escherichia coli cells Ptitsynm L. R.;G. Horneck;O. Komova;S. Kozubek;E. A. Krasavin;M. Bonev;P. Rettberg
  14. Mutat. Res. v.389 The Vitotox test an SOS bioluminescence Salmonella typhimurium test to measure genotoxicity kinetics van der Lelie D.;L. Regniers;B. Borremans;A. Provoost;L. Verschaeve https://doi.org/10.1016/S1383-5718(96)00158-1
  15. Appl. Env. Microbiol. v.63 Detection of DNA damage by use of Eschrichia coli carrying recA'::lux, uvrA'::lux, or AlkA'::lux reporter plasmids Vollmer A. C.;S. Belkin;D. R. Smulski;T. K. van Dyk;R. A. LaRossa
  16. Mutat. Res. v.466 Improved bacterial SOS promoter::lux fusions for genotoxicity detection Yaakov D.;R. Rozen;D. R. Smulski;T. K. van Dyk,A. C. Vollmer;D. A. Elsemore;R. A. Larossa;S. Belkin https://doi.org/10.1016/S1383-5718(99)00233-8