Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Identification and Characterization of the Vitro vulnificus Phosphomannomutase Gene

  • Lee, Jeong-Hyun (Department of Food Science and Technology, Department of Molecular Biotechnology, Instiute of Biotechnology Chonnam National University) ;
  • Park, Na-Young (Department of Food Science and Technology, Department of Molecular Biotechnology, Instiute of Biotechnology Chonnam National University) ;
  • Park, Soon-Jung (Department of Parasitology and Institute of Tropical, Yonsei University College of Medicine) ;
  • Choi, Sang-Ho (Department of Food Science and Technology, Department of Molecular Biotechnology, Instiute of Biotechnology Chonnam National University)
  • Published : 2003.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

Numerous virulence factors such as O antigen have been proposed to account for the fulminating and destructive nature of V. vulnificus infections. To better characterize the role of O antigen, a pmm gene encoding a phosphomannomutase was identified and cloned from V. vulnificus. The deduced amino acid sequence of the pmm was 42 to 71% similar to that reported from other Enterobacteriaceae. Functions of the pmm gene in virulence were assessed by the construction of an isogenic mutant, whose pmm gene was inactivated by allelic exchanges, and by evaluating its phenotype changes in vitro and in mice. The disruption of pmm resulted in a loss of more than 90% of phosphomannomutase, and reintroduction of recombinant pmm could complement the decrease of phosphomannomutase activity, indicating that the pmm gene encodes the phosphomannomutase of V. vulnificus. There was no difference in the $LD_50S$ of the wild-type and the pmm mutant in mice, but the $LD_50S$ observed by the mutant complemented with recombinant pmm were lower. Therefore, it appears that PMM is less important in the pathogenesis of V. vulnificus than would have been predicted by examining the effects of injecting purified LPS into animals, but it is not completely dispensable for virulence in mice.

Keywords

References

  1. Cell v.109 Small talk: Cell-to-cell communication in bacteria Bassler B. L. https://doi.org/10.1016/S0092-8674(02)00749-3
  2. Annu. Rev. Microbiol. v.34 Diseases of humans (other than cholera) caused by vibrios Blake P. A.;R. E. Weaver;D. G. Hollis https://doi.org/10.1146/annurev.mi.34.100180.002013
  3. Analytical Biochemistry v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding Bradford M. M. https://doi.org/10.1016/0003-2697(76)90527-3
  4. Infect. Immun. v.59 Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector Donnenberg M. S.;J. B. Kaper
  5. Microb. Pathog. v.13 Reversal of hypotension by Vibrio vulnificus lipopolysaccharide in the rat by inhibition of nitic oxide synthetase Elmore S. P.;J. A. Watts;L. M. Simpson;J. D. Oliver https://doi.org/10.1016/0882-4010(92)90082-Y
  6. Ann. Surg. v.223 A role for tumor necrosis factor-alpha in the increased mortality associated with Vibrio vulnificus infection in the presense of hepatic dysfunction Espat N. J.;T. Auffenberg;A. Abouhamze;J. Baumhofer;L. L. Moldawer;R. J. Howard https://doi.org/10.1097/00000658-199604000-00012
  7. Rev. Infect. Dis. v.10 Molecular Kochs postulates applied to microbial pathogenicity Falkow S. https://doi.org/10.1093/cid/10.Supplement_2.S274
  8. Annu. Rev. Microbiol. v.50 Census and consensus in bacteria ecosystems: the luxR-LuxI family of quorum-sensing transcriptional regulators Fuqua C.;S. C. Winans;E. P. Greenberg https://doi.org/10.1146/annurev.micro.50.1.727
  9. J. Bacteriol. v.175 Synthesis of lipopolysaccharide o side chains by Pseudomonas aeruginosa PAO1 requires the enzyme phosphomannomutase Goldberg J. B.;K. Hanato;G. B. Pier
  10. J. Biol. Chem. v.276 Differential expression of Vibrio vulnificus elastase gene in a growth phase-dependent manner by two different types of promoters Jeong H. S.;K. C. Jeong;H. K. Choi;K. J. Park;K. H. Lee;J. H. Rhee;S. H. Choi
  11. Infect. Immun. v.68 Construction and phenotypic evaluation of Vibrio vulnificus vvpE mutant for elastolytic protease Jeong K. C.;H. S. Jeong;J. H. Rhee;S. E. Lee;S. S. Chung;A. M. Starks;G. M. Escudero;P. A. Gulig;S. H. Choi https://doi.org/10.1128/IAI.68.9.5096-5106.2000
  12. Gene v.70 Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria Keen N. T.;S. Tamaki;D. Kobayashi;D. Trollinger https://doi.org/10.1016/0378-1119(88)90117-5
  13. J. Microbiol. Biotechnol. v.12 Identification and fuctional analysis of the putAP genes encoding Vibrio vulnificus proline dehydrogenase and proline permease Kim H. J.;J. H. Lee;J. E. Rhee;H. S. Jeong;H. K. Choi;H. J. Chung;S. Ryu;S. H. Choi
  14. Ann. Intern. Med. v.109 Syndromes of Vibrio vulnificus infections. Clinical and epidemiologic features in Florida cases, 1981-1987 Klontz K. C.;S. Lieb;M. Schreiber;H. T. Janowski;L. M. Baldy;R. A. Gunn https://doi.org/10.7326/0003-4819-109-4-318
  15. J. Microbiol. Biotechnol. v.11 Enumeration of Vibrio vulnificus in natural samples by colony blot hybridization Lee J. H.;K. H. Lee;S. H. Choi
  16. Mol. Microbiol. v.8 Chromosomal mapping, expression and synthesis of lipopolysaccharide in Pseudomonas aeruginosa: A role guanosine diphospho(GDP)-D-mannose Lightfoot J.;J. S. Lam https://doi.org/10.1111/j.1365-2958.1993.tb01620.x
  17. Trends Microbiol. v.29 Pseudomonas aeruginosa: Genes and enzymes of alginate synthesis May T. B.;A. M. Chakrabarty
  18. Microbios. v.67 Physiological effects of the lipopolysaccharide of Vibrio vulnificus on mice and rats McPherson V. L.;J. A. Watts;L. M. Simpson;J. D. Oliver
  19. Annu. Rev. Microbiol. v.55 Quorum sensing in bacteria Miller M. B.;B. B. Bassler https://doi.org/10.1146/annurev.micro.55.1.165
  20. J. Bacteriol. v.170 A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR Miller V. L.;J. J. Mekalanos
  21. J. Microbiol. Biotechnol. v.12 Involvement of lipopolysaccharide of Bradyrhizobium japonicum in metal binding Oh E. T.;H. S. Yun;T. R. Heo;S. C. Koh;K. H. Oh;J. S. So
  22. J. Mol. Biol. v.147 Nucleotide sequence of the kanamycin resistance transposon Tn903 Oka A.;H. Sugisaki;M. Takanami https://doi.org/10.1016/0022-2836(81)90438-1
  23. Am. J. Hyg. v.27 A simple method of estimating fifty percent endpoints Reed L. J.;H. Muench
  24. FEMS Microbiol. Lett. v.208 Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress Rhee J. E.;J. H. Rhee;P. Y. Ryu;S. H. Choi https://doi.org/10.1111/j.1574-6968.2002.tb11089.x
  25. Gene v.262 Identification and characterization of an in vivo regulated D15/Oma87 homologue in Shigella flexneri using differential display polymerase chain reaction Robb C. W.;C. J. Orihuela;M. B. Ekkelenkamp;D. W. Niesel https://doi.org/10.1016/S0378-1119(00)00537-0
  26. J. Bacteriol. v.169 Alginate biosynthetic enzymes in mucoid and nonmucoid Pseudomonas aeruginosa: Overproduction of phosphomannose isomerase, phosphomannomutase and GDP-mannose pyrophosphorylase by overexpression of the phosphomannose isomerase (pmi) gene Sa Correia I.;A. Darzins;S. K. Wang;A. Berry;A. M. Chakrabarty