Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Signal Transduction-related Gene Expression Analysis in MCF-7 followed by $\gamma$-radiation

MCF-7 세포주에서$\gamma$선에 의한 세포신호 전달 관련 유전자의 발현 양상의 분석

  • 박지윤 (한양대학교 생화학 및 분자생물학과) ;
  • 황창일 (서울대학교 의과대학 생화학교실) ;
  • 박웅양 (서울대학교 의과대학 생화학교실) ;
  • 김진규 (한국원자력연구소 동위원소 방사선 응용팀) ;
  • 채영규 (한양대학교 생화학 및 분자생물학과)
  • Published : 2003.03.01
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Abstract

There is considerable evidence that ionizing radiation (IR) mediates checkpoint control, repair and cell death. In this study, we have used a high density microarray hybridization approach to characterize the transcriptional response of human breast carcinoma MCF-7 cell line to ${\gamma}$-radiation, such as 4 Gy 4 hr, 8 Gy 4 hr, and 8 Gy 12 hr. We found that exposure to ${\gamma}$-ray alters by at least a $log_2$ factor of 1.0 the expression of 115 known genes. Of the 66 genes affected by ${\gamma}$-radiation, 49 are down-regulated. In our results, the cellular response to irradiation includes induction of the c-jun and EGR1 early response genes. The present work has examined potential cytoplasmic signaling cascades that transduce IR-induced signals to the nucleus. 40S ribosomal protein s6 kinase modulates the activities of the mitogen activated protein kinase (MAPK) and c-Jun $NH_2$-terminal kinase (JNK1) cascades in human monocytic leukemia (U937/pREP4) cells. 14-3-3 family members are dimeric phosphoserine -binding proteins that participate in signal transduction and checkpoint control pathways.

Keywords

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