Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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In Vitro Immunopotentiating Activity of Cellular Components of Lactococcus lactis ssp. lactis

  • Kim, Ji Yeon (Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Seong-Kyu (Department of Applied Biological Chemistry, The University of Tokyo) ;
  • Ciiimura, Satoshi-Ha (Department of Applied Biological Chemistry, The University of Tokyo) ;
  • Kaminogawa, Shuichi (Department of Applied Biological Chemistry, The University of Tokyo) ;
  • Lee, Hyong-Joo (Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University)
  • Published : 2003.04.01
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Abstract

To determine the effect of immunopotentiating activity of cellular components of Lactococcus lactis ssp. lactis, the immune function was analyzed in vitro using mice cells. When stimulated with mitogens, productions of $IFN-{\gamma}$, IL-12, $TNF-{\alpha}$, and IL-6 were enhanced in spleen cells treated with cellular components, with IL-4 production being the highest in spleen cells treated with cytoplasm fraction. Without mitogen stimulation, the productions of $IFN-{\gamma}$ and IL-12 were the highest in spleen cells treated with heat-killed whole cell. $TNF-{\alpha}$ and IL-6 productions were also high in spleen cells treated with all cellular components. Only heat-killed whole cell showed significant enhancement in natural killer cell activity. In peritoneal exudates cells, $TNF-{\alpha}$ production was enhanced significantly by all cellular components of Lactococcus lactis ssp. lactis These results indicate that the cellular components of Lactococcus lactis ssp. lactis are capable of stimulating immune cells to produce cytokines, and that both their cell walls and cytoplasm fraction contribute to these capacities.

Keywords

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