Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Constructions of bft-k and t-3 Deficient Mutants of Bacteroides fragilis: Possible Role for Metalloprotease in Pathogenesis

  • Lee, Gun-Young (Department of microbiology, National Institute of Health) ;
  • Rhie, Gi-Eun (Department of microbiology, National Institute of Health) ;
  • Chung, Gyung-Tae (Department of microbiology, National Institute of Health) ;
  • Sung, Won-Keun (Department of microbiology, National Institute of Health) ;
  • Oh, Hee-Bok (Department of microbiology, National Institute of Health)
  • Published : 2003.04.01
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Abstract

We earlier reported the identification of bif-k, t-3, and a third ORF from an enterotoxigenic strain of Bacteroides fragilis 419, which was isolated from the blood of a Korean patient suffering from systemic infections. In the present study, the deleted fragments of the t-3 and the bft-k genes from B. fragilis 419 were cloned into suicide vector PJST55 and used to create a mutant with chromosomal disruption of the t-3 and bft-k genes. Structures of the selected mutants, DMP-2 and DBT-4, were found to be intermediate forms that integrated the suicide vector into the chromosome. t-3 disrupted Dmp-2 and Bft-k disrupted DBT-4 did not react with polyclonal antibodies against T-3 or BFT-K, and had no biological activity in $HT29/C_1$, cells.

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