Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Demonstration of Two Independent Dextranase and Amylase Active Sites on a Single Enzyme Elaborated by Lipomyces starkeyi KSM 22

  • LEE, SO-YOUNG (Department of Material and Biochemical Engineering, Chonnam National University) ;
  • JIN-HA LEE (Engineering Research Institute, Chonnam National University) ;
  • JOHN F. ROBYT (Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, USA) ;
  • EUN-SEONG SEO (Department of Material and Biochemical Engineering, Chonnam National University) ;
  • HYEN-JOUNG PARK (Department of Material and Biochemical Engineering, Chonnam National University) ;
  • DOMAN KIM (Department of Material and Biochemical Engineering, Chonnam National University)
  • Published : 2003.04.01
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Abstract

Lipomyces starkeyi KSM 22 elaborates an enzyme that has both dextranase and amylase activities in a single protein of 100 kDa. Competition studies, using different amounts of dextran and starch as substrates, gave a competition plot consistent with the hypothesis that the hydrolysis of dextran and starch occurs at two independent active sites, each specific for starch and dextran, respectively.

Keywords

References

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