Hematological Changes of Olive Flounder, Paralichthys Olivaceus Exposed to Aroclor 1254

Aroclor 1254에 노출된 Olive flounder, Paralichthys of olivaceus의 혈액학적 변동

  • 김재원 (부경대학교 수산생명의학과) ;
  • 지정훈 (부경대학교 수산생명의학과) ;
  • 홍수희 (부경대학교 수산생명의학과) ;
  • 강주찬 (부경대학교 수산생명의학과)
  • Published : 2003.06.01

Abstract

Effects of Aroclor 1254, on hematological disturbance were investigated in the olive flounder, Paralichthys olivaceus. Flounders were exposed to waterborne PCB (3.0${\mu}g \; L^{-1}$) for 00 days. Blood samples were taken to determine their hematological disturbances. Plasma calcium concentration was significantly decreased in PCBs exposed flounder compared to the control group, while magnesium and inorganic phosphorus concentrations in PCBs exposed flounders were not significantly influenced during the test Belied. Although plasma total protein and albumin level were significantly reduced compared to the control group after 40 days, plasma glucose level was found to be significantly increased over the control group. Exposure to waterborne PCBs resulted in significant increase in the various enzyme activities, such as GOT, GPT and ALP in the flounder. The results of the present study led us to conclude that concentration of PCBs 3.0${\mu}g \; L^{-1}$ of the estuarine could markedly affect the hems tological distribution of olive flounder, Paralichthys olivaceus.

$PCB_{s}$ 농도 3.0${\mu}g \; L^{-1}$ 노출에 따른 넙치의 혈액학적 변동을 조사하기 위하며 60일간 침지실험을 실시하였다. PCBs에 노출된 넙치의 무기성분 중에 혈장 magnesium과 inorganic phosphorus는 유의적 변동이 없었으나, calcium은 대조구에 비해 유의한 감소를 보였다. 또 한 유기성분 중에 total protein과 albumin은 노출 20일 째부터 유의한 감소를 보여 실험 종료시가지 이러한 감소는 지속되었고, glucose는 노출 40일 이후 유의한 증가를 나타내었다. 혈장 ALP와 GPT의 활성도는 노출 20 일 이후부터 대조구에 비해 유의한 증가를 나타내었고, GOT는 노출 40일 이후 유의한 증가를 나타내었다. 이상의 결과는 해양에 3.0${\mu}g \; L^{-1}$의 PCBs농도가 존재할 경우 넙치의 혈액학적 변동을 야기할 수 있음을 의미한다.

Keywords

References

  1. Assessment Report;Arctic Pollution Issues Arctic Monitoring and Assessment Program AMAP
  2. Mutagenesis v.11 no.5 Cytogenetic studies of PCB77 on brown trout(Salmo trutta fario) using the micronucleus test and the alkaline comet assay Belpaeme,K.;K,Delbeke;L.Zhu;M.Kirsch-Volders https://doi.org/10.1093/mutage/11.5.485
  3. Nature v.395 Accumulation of persistent organochlorine compounds in mountains of western Canada Blais,J.M.;D.M.Schindler;D.C.G.Muir;L.E.Kimpe;D.B.Donald;B.Rosenberg https://doi.org/10.1038/26944
  4. Helgoland Marine Res. v.53 The use of fish metabolic pathological and parasitological indices in pollution monitoring. I. North Sea. Broeg,K.;S.Zander;A.Diamant;G.Kruner;I.Paperna;H.V.Westernhagen https://doi.org/10.1007/s101520050023
  5. J. Fish Dis. v.8 no.5 Serum chemistry of diseased English sole, Parophrys vetulus Girard, from polluted areas of Puget Sound, Washington Casillas,E.;W.Ames https://doi.org/10.1111/j.1365-2761.1985.tb01277.x
  6. Environ. Health Perspect. v.107 Potential mechanisms of thyroid disruption in humans;interaction of organochlorine compounds with thyroid receptor, transthyretin, and thyroid-binding globulin Cheek,A.O.;K.Kow;J.Chen;J.A.McLachlan
  7. Chemically-induced Alterations in Sexual and Functional Development;The Wildlife/Human Connection v.XXI Colborn,T.(ed.);C.Clement(ed.)
  8. Mar. Env. Res. v.54 Application of Real time PCR determination to assess interanimal variabilities in CYP1A induction in the European flounder(Platichthys flesus) Dixon,T.J.;J.B.Taggart;S.G.George https://doi.org/10.1016/S0141-1136(02)00189-7
  9. Environ. toxicol. Chem. v.12 Effects of chemical contaminants on blood chemistry of teleost fish;A bibliography and synopsis of selected effects Folmar,L.C. https://doi.org/10.1897/1552-8618(1993)12[337:EOCCOB]2.0.CO;2
  10. Uttar Pradesh J. Zool v.7 Impact of commercial grade hexagor and sumidon on behaviour and some aspects of carbohydrate metabolism in fish Cyprinus carpio Ghosh,T.K.
  11. Environ. Health. Perspect. v.102 Developmental abnormalities of the gonad and abnomal sex hormone concentrations in juvenile alligators from contaminated and control lakes in Florida Guillette,L.J.;T.S.Gross;G.R.Masson;J.M.Matter;H.F.Percival;A.R.Woodward https://doi.org/10.2307/3432198
  12. Acta Pharmac. Tox. v.35 Malathion induced biochemical changes in rats Gupta,P.K.
  13. Toxicol. Lett. v.15 The effects of a few xenobiotics on certain phosphates in the plasma of Clarias batrachus and Cirrhina mrigala Gupta,A.K.;S.S.Dhillon https://doi.org/10.1016/0378-4274(83)90213-8
  14. Environ. Health Perspect. v.106 Stepping backward to improve assessment of PCB congeners toxicities Hansen,L.G. https://doi.org/10.2307/3433919
  15. Environ. Toxicol. Chem. v.11 no.11 Effects of bleached kraft mill effluent on fish in the St. Maurice River, Quebec Hodson,P.V.;M.Mcwhirter;K.Ralph;B.Gray;D.Thivierge;J.H.Carey;G.Van-Der-Kraak;D.M.Whittle;M.C.Levesque https://doi.org/10.1897/1552-8618(1992)11[1635:EOBKME]2.0.CO;2
  16. Bull. Jap. Soc. Sci. Fish v.46 no.4 Changes in glucose, protein contents and enzyme activities of serum in carp administered orally with PCB Ito,Y.;T.Murata
  17. Bull. Jap. Soc. Sci. Fish v.43 no.5 Effects of oral administration of cadmium on fish-Ⅰ. Analytical results of the blood and bones Jiro,K.;I.Yasuo https://doi.org/10.2331/suisan.43.523
  18. Pak. J. Zool v.28 no.1 Effect of malathion on blood parameters of the fish, Cyprinion watsoni Khattak,I.U.D.;M.A,Hafeez
  19. Rev. Fish. Biol. Fish. v.4 The effects of pollution on reproduction in fish Kime,D.E.
  20. Occup. Environ. Med. v.54 Cancer mortality among electric utility workers exposed to polychlorinated biphenyls Loomis,D.;S.R.Browning;A.P.Schenck;E.Gregory;D,Savitz https://doi.org/10.1136/oem.54.10.720
  21. Am. fish. Soc. v.114 Waste transformer oil and PCB toxicity to rainbow trout Mayer,K.S.;F.L.Mayer,;A.Witt https://doi.org/10.1577/1548-8659(1985)114<869:WTOAPT>2.0.CO;2
  22. Fish Physioloty Chemical properties of the blood McDonald,D.G.;C.L.Milligan;Hoar,W.S.(ed.);D.J.Randall(ed.);A.P.Farrell(ed.)
  23. Elsevier Estrogens in the environment Ⅱ McLachlan,J.A.(Ed.)
  24. Fish Shellfish Immunol. v.13 no.1 In vitro toxicity and interations of environmental contaminants(Arochlor 1254 and mercury) and immunomodulatory agents(lipopoly saccharide and cortisol) on thymocytes from lake trout(Salvelinus namaycush) Miller,G.G.;L.I.Sweet;J.V.Adams;G.M.Omann;D.R.Passino-Reader;P.G.Meier https://doi.org/10.1006/fsim.2001.0381
  25. Handbook of ecotoxicology v.2 PCBs, PCDDs and PCDFs Mimmi,A.J.;Calow,P.(ed.)
  26. Comp. Biochem. Physiol. v.103C Differential effects of 3,4,5,3'.4',5'-hexachlorobipheny(HCB) on interrenal steroidogenesis in male and female rainbow trout, Oncorhynchus mykiss Miranda,C.L.;M.C.Henderson;J.L.Wang;H.A.Chang;H.D.Hendricks;D.R.Buhler
  27. Environ. Sci. Technol. v.22 Organochlorine Contaminants in arctic food chains; Accumulation of specific polychlorinated biphenyls and chlordanerelated compounds Muir,D.C.G.;R.J.Norstrom;M.Simon https://doi.org/10.1021/es00174a012
  28. Environ. Int. v.26 Tissue distribution and temperature-dependence of Anguilla anguilla L.EROD activity following exposure to model inducers and relationship with plasma cortisol, lactate and glucose levels Pacheco,M.;M.A.Santo https://doi.org/10.1016/S0160-4120(00)00101-X
  29. J. Nat. Conserv. v.2 no.2 Histopathological and biochemical changes in the liver of a fresh water fish exposed to heptachlor Rao,P.P.;K.V.Joseph;K.J.Rao
  30. Bull. Environ. Contam. Toxicol. v.49 Changes in erythropoietic activity of Sarotherodon mossambicus exposed to sublethal concentration of the herbicide diuron Reddy,D.C.;P.Vijayakumari;V.Kalarani;W.Davis Ronald
  31. J. Fish. Res. Board Can. v.36 no.11 Relationship of cadmium-induced hypocalcemia with mortality in rainbow trout(Salmo gairdneri)and the influence of temperature on toxicity Roch,M.;E.J.Maly
  32. Environ. Health Perspect v.103 no.SUP.4 Environmentally induced alterations in development; a focus on wildlife Rolland,R.;M.Gilbertson;T.Colborn(eds.)
  33. Chemically-Induced Alterations in Functional Development and Reproduction of Fishes Rolland,R.M.(ed.);M.Gilvertson(ed.);R.E.Peterson(ed.)
  34. Bull. Jap. Soc. Sci. Fish. v.44 no.3 Requirement of red sea bream for dietary iron.Ⅱ. Sakamoto,S.;Y.Yone https://doi.org/10.2331/suisan.44.223
  35. Mar. Environ. Sci. v.16 no.1 Effects of petroleum on the sero-proteins of Tilapia mossambica Shen,H.;Q.Zhang;R.Xu;G.Wang
  36. J. Fish. Biol. v.11 Changes of blood enzymes in brook trout induced by infection with Aeromonas salmonicida Shich,M.S.
  37. J. Fish. Biol. v.13 Some histopathological effects of Aroclor 1254 in the liver and gonads of rainbow trout, Salmo gairdneri and carp, Cyprinus carpio Sivarajah,K.;C.S.Franklin;W.P.Williams https://doi.org/10.1111/j.1095-8649.1978.tb03448.x
  38. J. Fish. Biol. v.17 Automated chemical analysis in fish health assessment Smith,A.C.;F.Ramos https://doi.org/10.1111/j.1095-8649.1980.tb02777.x
  39. Mar. Environ. Res. v.46 no.1-5 Assessment of environmental quality and inland water pollution using biomarker responses in caged carp(Cyprinus carpio): Use of a bioactivation : detoxication ratio as a biotransformation index(BTI) Van der Oost,R.;S.C.C.Lopes;H.Homen;K.Satumalay;R.Van der Bos;H.Heida;N.P.E.Vermeulen https://doi.org/10.1016/S0141-1136(97)00096-2
  40. Bull. Jap. Soc. Sci. Fish. v.52 no.3 Hemochemical changes in carp exposed to low cadmium concentrations Yamawaki,K.;W.Hashimoto;K.Fujii;J.Koyama;Y.Ikeda;H.Ozaki https://doi.org/10.2331/suisan.52.459