Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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In vitro Response of Cytochrome P450 (CYP) and EROD Activity in Bivalve (Coelomactra antiquata) by DDT, PCB-153 and TCDD

유기염소화합물인 DDT, PCB-153 및 TCDD에 in vitro 노출시킨 명주조개 미크로좀의 CYP및 EROD활성 반응

  • Published : 2003.06.01
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Abstract

The effect of xenobiotics on cytochrome P450 (CYP) and 7-ethoxyresorufin-O-deethylase (EROD) in hepatopancreatic microsome of surf clam, Coelomactra anti-quata, were investigated. The microsome isolated from the digestive gland of the surf clam, collected from the east coast of Korea, was in vitro exposed to p, p -DDT (0.1,0.4 and 1.0 mM) for 30 min and 2,3,7,8-TCDD (0.01, 0.04 and 0.1 ppb) and PCB-153 (0.01, 0.04 and 0.1 ppb) for 7 hr. In the case of DDT exposure, the CYP content and EROD activity of 1.0 mM exposure group increased up to about 117% and 120% of the DMSO solvent control group after 10 min. exposure, respectively. After 2 hr exposure of TCDD, the CYP content and EROD activity were also induced to the range of 103∼110% and 121∼139%, respectively. The PCB-153 exposure group showed 107∼115% of CYP content and 129∼140% of EROD activity after 2 hr exposure. Three test chemicals apparently induced CYP and EROD activity in the microsome of surf clam. The inducing potentials depend en the test chemicals.

내분비계 장애물질이 해양생물에게 미치는 영향을 조사하기 위한 연구의 일환으로 우리나라 연안떼서 보편적으로 분포하는 p,p'-DDT, 2, 3, 7, 8-TCDD및 PCB-153을 대상으로 이들 화합물이 동해안산 명주조개의 미크로좀 중 약물대사 효소계에 미치는 형향을 살펴보았다. 명주조개의 중장선으로 만든 미크로좀에 상기한 화합물을 DMSO에 녹여 일정한 농토가 되도록 첨가하고 p,p'-DDT (0.1, 0.4 및 1.0 mM)는 30분까지 그리고 2, 3, 7, 8-TCDD (0.01, 0.04 및 0.1 ppb) 및 PCB-153 (0.01, 0.04및 0.1ppb)은 7시간까지 in vitro로 배양하면서 CYP 함량과 EROD 활성의 변화를 경시적으로 측정하였다. 그리고 용제인 DMSO만을 첨가한 미크로좀으로 sham구를 설정하였다. DDT와 배양한 경우, 0.4 및 1.0mM 배양구의 CYP농도는 노출 전에 비해 10분 후에 각각 115%와 117%로 높아졌고, EROD활성도 120%로 증가하였다. TCDD와 배양한 경우, CYP농도는 노출 전에 비해 9시간 후에 최대(103∼110%)가 되었고, BROD활성도 2시간 후에 최대(121∼139%)가 되었다. PCB-153와 배양한 경우, 0.01,0.04 및 0.10 ppb 배양구는 CYP농도가 9시간 후에 각각 107%, 112%및 112%로 최대가 되었고, EROD활성도 각각 129%,140% 및 135%로 최대가 되었다. 이것으로 세 화합물은 모두 중장선의 미크로좀에서 CYP와 ERDD수준을 높이는 유도효과가 있음을 확인할 수 있었다.

Keywords

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