Plant Terpenes Enhance Survivability of Polychlorinated Biphenyl (PCB) Degrading Pseudomonas pseudoalcaligenes KF707 Labeled with gfp in Microcosms Contaminated with PCB

  • Oh, Eun-Taex (Department of Biological Engineering and Center for Advanced Bioseperation Technology, Inha University) ;
  • Koh, Sung-Cheol (Division of Civil and Environmental Engineering, Korea Maritime University) ;
  • Kim, Eung-Bin (Department of Biology, Yonsei University) ;
  • Ahn, Young-Hee (Department of Environmental Engineering and Science, Kwangju Institute of Science & Technology) ;
  • So, Jae-Seong (Department of Biological Engineering and Center for Advanced Bioseperation Technology, Inha University)
  • 발행 : 2003.06.01

초록

Polychlorinated biphenyl are toxic pollutants and their degradation is quite slow in the environment. Recently, interest if bioremediation using PCB-degrading bacteria has increaset,. In a previous report, plant terpenes (p-cymene, (S)-(-)-limonene, ${\alpha}-pynene$, and ${\alpha}-terpinene$) have been found to be utilized by a PCB degrader and to induce the biphenyl dioxygenase gene in pure culture. In this study, Pseudomonas pseudoalcaligenes KF707, a PCB-degrading Gram-negative soil bacterium, was used to determine whether the terpene stimulation of PCB degrader occurred in the natural environment. First, P. pseudoalcaligenes KF707 was genetically tagged using a transposon with gfp (green fluorescent protein) as a reporter gone. The population dynamics of P. pseudoalcaligenes KF707 harboring gfp gene in a PCB-contaminated environment was examined with or without terpenoids added to the microcosm. About 10-100-fold increase was found in the population of PCB degraders when terpene was added, compared with control (non-terpenes samples and biphenyl added samples). It was proposed that the gfp-monitoring system is very useful and terpenes enhance the survivability of PCB degraders in PCB-contaminated environments.

키워드

참고문헌

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