The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification and Characterization of New Copia-like Retrotransposon Osr1 in Rice

  • Lee, Yong-Hwan (Department of Molecular Biology, College of Life Science, Sejong University) ;
  • Jwa, Nam-Soo (Department of Molecular Biology, College of Life Science, Sejong University) ;
  • Park, Sook-Young (School of Agricultural Biotechnology, Seoul National University) ;
  • Park, Chan-Ho (School of Agricultural Biotechnology, Seoul National University) ;
  • Han, Seong-Sook (Plant Pathology Division, National Institute of Agriculture Science and Technology)
  • Published : 2003.02.01
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Abstract

An insertion sequence identified as a solo long terminal repeat (LTR) of a new rice copia-like retrotransposon was detected in the ORE of the Pi-b gene from the rice cv. Nipponbare, and was designated as Osr1. Osr1 consists of a 6386 bp nucleotide sequence including 965 bp LTRs on both ends with an 82％ nucleotide sequence identity to the wheat Tarl retrotransposon on reverse transcriptase. Nucleotide divergence was noted among the individual LTRs, as well as the coding region of Osr1. Various restriction fragment length polymorphism (RFLP) of LTR were detected in indica cultivars, whereas, only a few could be detected in the japonica cultivars. The population of Osr1 is lower in the wild-type rice compared with that in the domesticated cultivars. The insertion of LTR sequence in the h-b gene in the susceptible cultivar suggested that retro-tyansposon-mediated insertional mutation might play an important role in the resistance breakdown, as well as in the evolution of resistance genes in rice.

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