$F(ab)_2$-ELISA for the Detection of Nuclear Polyhedrosis Virus of Silk-worm, Bombyx mori L.

  • Sivaprasad, V. (Central Sericultural Research & Training Institute (CSRTI), Mysore 570 008, India.) ;
  • Nataraju, B. (Central Sericultural Research & Training Institute (CSRTI), Mysore 570 008, India.) ;
  • Baig, M. (Central Sericultural Research & Training Institute (CSRTI), Mysore 570 008, India.) ;
  • Samson, M.V. (Central Sericultural Research & Training Institute (CSRTI), Mysore 570 008, India.) ;
  • Datta, R.K. (Central Sericultural Research & Training Institute (CSRTI), Mysore 570 008, India.)
  • Published : 2003.06.01

Abstract

$F(ab`)_2$-ELISA and direct antigen coating-ELISA (DAC-ELISA) were evaluated in the detection of purified Bombyx mori nuclear polyhedrosis virus (BmNPV) and nuclear polyhedrosis virus infection in silkworm larvae inoculated with BmNPV polyhedra. Although nanogram levels of BmNPV was detected in both DAC- and $F(ab`)_2$-ELISA, similar concentrations of antigen was detected in case of F(ab’)$_2$-ELISA even at higher dilution of antibody (up to 1 : 20 K). One hundred percent nuclear polyhedrosis infection was detected 6 hrs after inoculation in BmNPV infected silkworm larvae by $F(ab`)_2$-ELISA. On the other hand, detection of 100% infection was observed only three days after inoculation in DAC-ELISA. In this study, it was observed $F(ab`)_2$-ELISA was more sensitive than DAC-ELISA in the detection of purified BmNPV as well as nuclear polyhedrosis infection in silkworm larvae.

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