Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Effects of anti-allergic drugs on intestinal mastocytosis and worm expulsion of rats infected with Neodiplostomum seoulense

  • Shin, Eun-Hee (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Kim, Tae-Heung (Department of Dermatology, College of Medicine, Gyeongsang National University) ;
  • Hong, Sung-Jong (Department of Parasitology, Chung-Ang University College of Medicine) ;
  • Park, Jae-Hwan (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Guk, Sang-Mee (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center)
  • Published : 2003.06.01
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Abstract

The effects of anti-allergic drugs on intestinal mastocytosis and the expulsion of Neodiplostomum seoulense were observed in Sprague-Dawley rats, after oral infection with 500 metacercariae. The drugs used were hydroxyzine (a histamine receptor H$_1$ blocker), cimetidine (a H$_2$ blocker), cyclosporin-A (a helper T-cell suppressant), and prednisolone (a T- and B-cell suppressant). Infected, but untreated controls, and uninfected controls, were prepared. Worm recovery rate and intestinal mastocytosis were measured on weeks 1, 2, 3, 5, and 7 post-infection. Compared with the infected controls, worm expulsion was significantly (P < 0.05) delayed in hydroxyzine- and cimetidine-treated rats, despite mastocytosis being equally marked in the duodenum of all three groups. In the cyclosporin-A- and prednisolone-treated groups, mastocytosis was suppressed, but worm expulsion was only slightly delayed, without statistical significance. Our results suggest that binding of histamine to its receptors on intestinal smooth muscles is more important in terms of the expulsion of N. seoulense from rats than the levels of histamine alone, or mastocytosis.

Keywords

References

  1. Chai JY, Kim TH, Kho WG, et al. (1993) Mucosal mast cell responses to experimental Metagonimus yokogawai infection in rats. Korean J Parasitol 31: 129-134.
  2. Chai JY, Kim TK, Cho WH, et al. (1998) Intestinal mastocytosis and goblet cell hyperplasia in BALB/c and C3H mice infected with Neodiplostomum seoulense. Korean J Parasitol 36: 109-119.
  3. Chai JY, Shin EH, Han ET, Guk SM, Choi MH, Lee SH (2000) Genetic difference in susceptibility and fatality of three strains of mice experimentally infected with Neodiplostomum seoulense. J Parasitol 86: 1140-1144.
  4. Dehlawi MS, Wakelin D, Behnke JM (1987) Suppression of mucosal mastocytosis by infection with the intestinal nematode Nematospiroides dubius. Parasite Immunol 9:187-194.
  5. Else KJ, Finkelman FD (1998) Intestinal nematode parasites, cytokines and effector mechanisms. Int J Parasitol 28:1145-1158.
  6. Fujino T, Fried B, Ichikawa H, Tada I (1996) Rapid expulsion of the intestinal trematodes Echinostoma trivolvis and E. caproni from C3H mice by trapping with increased goblet cell mucins. Int J Parasitol 26: 319-324.
  7. Fujino T, Ichikawa H, Fukuda K, Fried B (1998) The expulsion of Echinostoma trivolvis caused by goblet cell hyperplasia in severe combined immunodeficient (SCID) mice. Parasite 5: 219-222.
  8. Goldsmith P, Mcgarity B, Walls AF, et al. (1990) Corticosteroid treatment reduces mast cell numbers in inflammatory bowel disease. Dig Dis Sci 35: 1409-1413.
  9. Hong SJ, Lee SH, Seo BS, et al. (1983) Studies on intestinal trematodes in Korea IX. Recovery rate and development of Fibricola seoulensis in experimental animals. Korean J Parasitol 21: 224-233.
  10. Hong ST, Hong SJ, Lee SH, et al. (1982) Studies on intestinal trematodes in Korea VI. On the metacercaria and the second intermediate host of Fibricola seoulensis. Korean J Parasitol 20: 101-111.
  11. Hong ST (1982) Studies on intestinal trematodes in Korea VII. Growth, development and recovery of Fibricola seoulensis from experimentally infected rats and mice. Korean J Parasitol 20: 112-121.
  12. Huh S, Chai JY, Hong ST, et al. (1988) Clinical and histopathologic findings in mice heavily infected with Fibricola seoulensis. Korean J Parasitol 26: 45-53.
  13. King SJ, Miller HR, Newlands GF, Woodbury RG (1985) Depletion of mucosal mast cell protease by corticosteroids: effect on intestinal anaphylaxis in the rat. Proc Natl Acad Sci USA 82: 1214-1218.
  14. Kho WG, Chai JY, Chun CH, et al. (1990) Mucosal mast cell response to experimental Fibricola seoulensis infection in rats. Seoul J Med 31: 191-199.
  15. Kook J, Nawa Y, Lee SH, Chai JY (1998) Pathogenicity and lethality of a minute intestinal fluke, Neodiplostomum seoulense, to various strains of mice. J Parasitol 84: 1178-1183.
  16. Koyama K, Ito Y (2000) Mucosal mast cell responses are not required for protection against infection with the murine nematode parasite Trichuris muris. Parasite Immunol 22: 13-20.
  17. Lee SH, Yoo BH, Hong ST, et al. (1985) A Histopathological study on the intestine of mice and rats infected by Fibricola seoulensis. Korean J Parasitol 23: 58-72.
  18. Mary JM, Richard AH, Pamela CC (2000) Antihistamines; Lippincott's illustrated reviews. In Pharmacology. pp 420-421, Lippincott Williams & Wilkins, USA.
  19. McLauchlan PE, Roberts HC, Loxton NJ, Wastling JM, Newlands GF, Chappell LH (1999) Mucosal mast cell responses and release of mast cell protease-I in infections of mice with Hymenolepis diminuta and H. microstoma: modulation by cyclosporin A. Parasite Immunol 21: 151-61.
  20. Nawa Y, Ishikawa N, Tsuchiya K, et al. (1994) Selective effector mechanisms for the expulsion of intestinal helminths. Parasite Immunol 16: 333-338.
  21. Onah DN, Nawa Y (2000) Mucosal immunity against parasitic gastrointestinal nematodes. Korean J Parasitol 38: 209-236.
  22. Rothwell TL, Love RJ, Evans DP (1978) Studies on the role of histamine and 5-hydroxytryptamine in immunity against the nematode Trichostrongylus colubriformis. IV. Inhibition of the expulsion of worms transplanted into the duodenum of immune guinea pigs. Int Arch Allergy Appl Immunol 56: 457-462.
  23. Scott RB, Maric M (1993) Mediation of anaphylaxis-induced jejunal circular smooth muscle contraction in rats. Dig Dis Sci 38: 396-402.
  24. Seo BS (1990) Fibricola seoulensis Seo, Rim and Lee, 1964 (Trematoda) and fibricoliasis in man. Seoul J Med 31: 61-96.
  25. Starke WA, Oaks JA (2001) Ileal mucosal mast cell, eosinophil, and goblet cell populations during Hymenolepis diminuta infection of the rat. J Parasitol 87:1222-1225.
  26. Strobel S, Miller HR, Ferguson A (1981) Human intestinal mucosal mast cells: evaluation of fixation and staining techniques. J Clin Pathol 34: 851-858.
  27. Takeyama K, Tamaoki J, Nakata J, Konno K (1996) Effect of oxitropium bromide on histamine-induced airway goblet cell secretion. Am J Respir Crit Care Med 154: 231-236.
  28. Tamaoki J, Nakata J, Takeyama K, Chiyotani A, Konno K (1997) Histamine H2 receptor-mediated airway goblet cell secretion and its modulation by histaminedegrading enzymes. J Allergy Clin Immunol 99: 233-238.
  29. Weinstein MS, Fried B (1991) The expulsion of Echinostoma trivolvis and retention of Echinostoma caproni in the ICR mouse: pathological effects. Int J Parasitol 21: 255-257.
  30. Woodbury RG, Miller HRP, Huntly JF, et al. (1984) Mucosal mast cells are functionally active during spontaneous expulsion of intestinal nematode infections in rat. Nature 312: 450-452.