Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Structural Analysis of the fcbABC Gene Cluster Responsible for Hydrolytic Dechlorination of 4-Chlorobenzoate from pJS1 Plasmid of Comamonas sp. P08

  • Lee, Jeong-Soon (Department of Microbiology and Biotechnology, Chungbuk National University) ;
  • Lee, Kyoung (Department of Microbiology, Changwon National University) ;
  • Ka, Jong-Ok (Department of Agricultural Biology, Seoul National University) ;
  • Jong-Chan (Biotechnology Center Agriculture and Environment Rutgers University) ;
  • Kim, Chi-Kyung (Department of Microbiology and Biotechnology, Chungbuk National University)
  • Published : 2003.06.01
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Abstract

Bacterial strain No. P08 isolated from wastewater at the Cheongju industrial complex was found to be capable of degrading 4-chlorobenzoate under aerobic condition. P08 was identified as Comamonas sp. from its cellular fatty acid composition and 16S rDNA sequence. The fcb genes, responsible for the hydrolytic dechlorination of 4-chlorobenzoate, were cloned from the plasmid pJJl of Comamonas sp. P08. The fcb gene cluster of comamonas sp. PO8 was organized in the order fcbB-fcbA-fcbTl-fcbT2-fcbT3-fcbC. This organization of the fcb genes was very similar to that of the fcb genes carried on the chromosomal DNA of pseudomonas sp. DJ-12. However, it differed from the fcbA-fcbB -fcbC ordering of Arthrobacter sp. SU. The nucleotide sequences of the fcbABC genes of strain P08 showed 98% and 53% identities to those of Pseudomonas sp. DJ-12 and Arthrobacter sp. SU, respectively. This suggests that the fcb genes might have been derived from Pseudomonas sp. DJ-12 to form plasmid pJSl in Comamonas sp. P08, or that the fcb genes in strain DJ-12 were transposed from Comamonas sp. P08 plasmid.

Keywords

References

  1. Appl. Environ. Microbiol. v.61 A meta cleavage pathway for 4chlorobenzoate, an intermediate in the metabolism of 4-chlorobiphenyl by Pseudomonas cepacia P166 Arensdorf,J.J.;D.D.Focht
  2. Biochemistry v.31 Ancestry of the 4-chlorobenzoate dehalogenase: analysis of amino acid sequence identities among families of acyl : adenylligase, enoyl-CoA hydratases/isomerases, and acyl-CoA thioesterases Babbitt,P.C.;G.L.Kenyon;B.M.Martin;H.Charest;M.Sylvestre;J.D.Schoten;K.H.Chang;P.H.Liang;D.Dunaway Mariano https://doi.org/10.1021/bi00139a024
  3. Anal. Chem. v.29 Determination of trace amounts of chlorine in naphtha Bergmann,J.G.;J.Sanik https://doi.org/10.1021/ac60122a018
  4. J. Microbiol. v.35 Dechlorination of 4-chlorobenzoate by Pseudomonas sp. DJ-12 Chae,J.C.;C.K.Kim
  5. Gene v.258 Genetic structure and functional implication of the fcb gene cluster for hydrolytic dechlorination of 4-chlorobenzoate from Pseudomonas sp. DJ-12 Chae,J.C.;Y.S.Kim;Y.C.Kim;G.J.Zylstra;C.K.Kim https://doi.org/10.1016/S0378-1119(00)00419-4
  6. Biochemistry v.31 Isolation and characterization of the three polypeptide components of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS-3 Chang,K.H.;P.H.Liang;W.Beck;J.D.Scholten;D.Dunaway Mariano
  7. Adv. Appl. Microbiol. v.36 Microbial transformations of herbicides and pesticides Cork,D.J.;J.P.Krueger https://doi.org/10.1016/S0065-2164(08)70450-7
  8. Biodegaradation v.5 On the origins and functions of the enzymes of the 4-chlorobenzoate to 4-hydroxybenzoate converting pathway. Dunaway- Mariano,D.;P.C.Babbitt https://doi.org/10.1007/BF00696464
  9. Int. J. Syst. Bacteriol. v.41 Phylogenetic analysis of Aquaspirillum magnetotacticum using polymerase chain reaction-amplified 16S rDNA-specific DNA Eden,P.A.;T.M.Schmidt;R.P.Blakemore;N.R.Pace https://doi.org/10.1099/00207713-41-2-324
  10. Biodegradation v.5 Molecular genetics and evolutionary relationship of PCB-degrading bacteria Furukawa,K. https://doi.org/10.1007/BF00696466
  11. J. Bacteriol. v.183 Isolation and characterization of IS1409, an insertion element of 4-chlorobenzoatedegrading Arthrobacter sp. strain TM1, and development of a system fro transposon mutagenesis Gartemann,K.H.;R.Eichenlaub https://doi.org/10.1128/JB.183.12.3729-3736.2001
  12. FEMS Micriobiol. Rev. v.103 Microbial breakdown of halogenated aromatic pesticides and related compounds H$\ "{a}$ggblom,M.M. https://doi.org/10.1111/j.1574-6968.1992.tb05823.x
  13. Adv. Appl. Microbiol. v.37 Microbial degradation of biphenyl and its derivatives Higson,F.K. https://doi.org/10.1016/S0065-2164(08)70254-5
  14. Appl. Environ. Microbiol. v.62 Phenotypic and genotypic characterization of phenanthrene-degrading fluorescent Pseudomonas biovars Johnsen,K.;S.Andersen;C.S.Jacobsen
  15. J. Bacteriol. v.176 Nucleotide sequence and functional analysis of the meta-cleavage pathway involved in biphenyl and polychlorinated biphenyl degradation in Pseudomonas sp. strain KKS102 Kikuchi,Y.;Y.Yasukochi;Y.Nagata;M.Fukuda
  16. Biotechniques v.15 A rapid optimized protocol for downward alkaline southern blotting of DNA Koetsier,P.A.;J.Schorr;W.Doerfler
  17. Appl. Environ. Microbiol. v.58 Evidence for 4-chlorobenzoic acid dehalogenation mediated by plasmids related to pSS50 Layton,A.C.;J.Sanseverino;W.Wallace;C.Corcoran;G.S.Sayler
  18. Biochem. Biophy. Res. Commun. v.176 Dehalogenation of 4-chlorobenzoate by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3: an ATP/coenzyme A dependent reaction Loffler,F.;R.Muller;F.Lingens https://doi.org/10.1016/0006-291X(91)90398-Q
  19. Experiments in molecular genetics Miller,J.H.
  20. Ann. Rev. Microbiol. v.42 Microbial degradation of haloaromatics Reineke,W. https://doi.org/10.1146/annurev.mi.42.100188.001403
  21. Molecular cloning, A laboratory manual Sambrook,J.;E.F.Fritsch;T.Maniatis
  22. Proc. Natl. Acad. Sci. USA v.74 Sequencing with chain-terminating inhibitors Sanger,F.;S.Nicklen;A.R.Coulson https://doi.org/10.1073/pnas.74.12.5463
  23. J. Bacteriol. v.168 Cloning of Pseudomonas sp. strain CBS3 genes specifying dehalogenation of 4-chlorobenzote Savard,P.;L.Peloquin;M.Sylvestre
  24. Appl. Environ. Microbiol. v.58 Cloning and sequence analysis of genes for dehalogenation of 4-chlorobenzoate from Arthrobacter sp. strain SU. Schmitz,A.;K.H.Gartemann;J.Fielder;E.Grund;R.Eichenlaub
  25. Science v.253 Novel enzymic hydrolytic dehalogenation of a chlorinated aromatic Scholten,J.D.;K.H.Chang;P.C.Babbitt;H.Charest;M.Sylvestre;D.Dunaway-Mariano https://doi.org/10.1126/science.1853203
  26. FEMS Microbiol. Lett. v.41 Initial characterization of 4-chlorobenzoatedehalogenase from Pseudomonas sp. CBS2 Thiele,J.;R.Muller;F.Lingens
  27. FEMS Microbiol. Ecol. v.31 Phylogenetic and phenotypic dirversity of 4-chlorobenzoate-degrading bacteria isolated from soils Yi,H.R.;K.H.Min;C.K.Kim;J.O.Ka https://doi.org/10.1111/j.1574-6941.2000.tb00671.x
  28. Kor. J. Microbiol. v.30 Divergence of the cbp genes in 4CB catabolizing bacteria Youn,D.J;J.J.Han;C.K.Kim;Y.S.Kim