Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Isolation and Characterization of Bud6p, an Actin Interacting Protein, from Yarrowia lipolytica

  • Yunkyoung Song (Department of Microbiolog and Institute of Biotechnology, Chungnam National University) ;
  • Cheon, Seon-Ah (epartment of Microbiolog and Institute of Biotechnology, Chungnam National University) ;
  • Hwang, Ji-Sook (epartment of Microbiolog and Institute of Biotechnology, Chungnam National University) ;
  • Kim, Jeong-Yoon (epartment of Microbiolog and Institute of Biotechnology, Chungnam National University)
  • Published : 2003.06.01
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Abstract

The identification of genes involved in true hypha formation is important in the study of mechanisms underlying the morphogenetic switch in yeast. We isolated a gene responsible for the morphogenetic switch in Yarrowia lipolytica, which forms true hyphae in response to serum or N-acetylglucosamine. The isolated gene, encoding 847 amino acids, had sequence identities of 27% and 25% with the Bud6 (Aip3) proteins of Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. Disruption of this gene, designated YIBUD6, in haploid and diploid strains significantly reduced the ability of Y. lipolytica to switch from the yeast form to the hyphal form in hypha-inducing media. It was also found that YIBud6$\Delta$ mutants were rounder than the wild type when grown in the yeast form. These results indicate that the YIBud6 protein is necessary for hyphal growth and cell polarity in both haploid and diploid Y. lipolytica cells.

Keywords

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