Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Study on Anti-estrogenic Activity of DEHP as an Endocrine Disruption Chemical

내분비 교란성 DEHP의 항-에스트로젠 활성에 관한 연구

  • Kim, Eun-Joo (Digital Information Center for Environment Research, University of Pohang Science and Technology)
  • Published : 2003.06.01
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Abstract

Di-2-ethylhexyl phthalate (DEHP), is a widely used plasticizer known to be a suspected endocrine disrupter, but its exact effects on aquatic organisms are not yet known. When Japanese medaka (Oryzias latipes) were exposed from the time of hatching to 3 months of age to an aqueous DEHP solution at nominal concentrations of 1, 10, and 50 $\mu\textrm{g}$/l, DEHP treated female fish showed distinct reproductive effect. And the midge (Chironomus riparius.). an aquatic invertebrate, was exposed to DEHP to evaluate the effects on reproductive processes via sediment toxicity. The test endpoints included emergence, sex ratio, fecundity, and the viability of F1 offspring egg ropes. The result implied that the normal developmental and/or reproductive processes in C. riparius had been disrupted when exposed to DEHP, the effect also being displayed in the next generation. In summary, DEHP hinders the development of reproductive organs in the female Japanese medaka and C. riparius.

DEHP는 대표적인 플라스틱 가소제 가운데 하나로서, 광범위하게 사용되고 있으며, 내분비계 장애물질로 분류퇴고 있다. 실제로 하천, 해양, 토양 등 광범위한 환경에서 검출되고 있지만, 이 물질이 수서 생물에 미치는 내분비교란 영향과 기작에 대해서는 거의 알려진 바가 없다. 본 연구에서는 송사리로 불리우는 Oryzias latipes(Japanese medaka)와, 유생 시기에 저니성 무척추 동물로 존재하다가 성충이 되는 Chironomus riparius를 대상으로 DEHP가 내분비 장애물질로서 생식작용에 미치는 영향에 대하여 연구하였다. 먼저, Japanese medaka를 부화 직후부터 3개월간 DEHP 1, 10, 50 $\mu\textrm{g}$/l의 농도로 노출시킨 결과 암놈의 혈중 비텔로제닌의 감소 ,생식소 지수인 GSI(Gonado Somatic Index) 감소. 난자 발달 저해 등이 관찰되었다. 또한, C. riparius를 산란 직후부터 DEHP에 노출시킨 경우에는 성체 출현률, 암수 비율, 산란률에서는 용량-반응 관계를 가진 변화가 발견되지 않았으나, 산란된 알의 부화율은 DEHP에 노출된 경우 유의하게 감소하는 것이 관찰되었다. 이러한 결과를 종합하여 볼 때. DEHP는 O. latpes와 C. riparius 모두 생식 작용에 영향을 미치며, 그 작용 기작은 일반적으로 발견되는 에스트로젠(estrogen) 활성이 아닌 암놈의 생식기관의 발달을 직, 간접적으로 저해함으로서 정상적인 알의 생성을 방해하는 이른바 항-에스트로젠 기작을 보이는 것으로 추정된다. 본 연구에서는 DEHP 위해성 평가를 위한 기본 자료로서, 생식작용 영향에 대한 새로운 자료를 제시하였다.

Keywords

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