경북 동해안 환경에서 분리된 V. parahaemolyticus 및 V. vulnificus의 생태학적 및 항생제 감수성 특성

Environmental and Antimicrobial Characteristics of Vibrio spp. Isolated from Fish, Shellfish, Seawater and Brackish water samples in Gyeongbuk Eastern Coast

  • 손진창 (경상북도 보건환경연구원) ;
  • 박승우 (경상북도 보건환경연구원) ;
  • 민경진 (계명대학교 공중보건학과)
  • 발행 : 2003.06.01

초록

This study was carried out to investigated the distribution and characteristics of Vibrio spp. isolated from fish and shellfish, seawater and brackish water samples collected from Pohang, Uljin, Yeongduk and Gyeongju in Gyeongbuk Province from April 2000 to October 2000. Total 155 strains of genus Vibrio were isolated from 439 collected samples, and numbers of isolated strains of V. parahaemolyticus and V. vulnificus were 140 and 15, respectively. The isolation rate from the samples collected in Pohang was the highest as 41.5% (76 strains), and wat the highest as 71.4% (30 strains) in brackish water, and was the highest as 55.7% (34 strains) in August. And the optimal pH, temperature, and NaCl concentration for growth of V. parahaemolyticus and V. cholerae were 8.0, 3$0^{\circ}C$ and 2.0%, respectively. In a resistance test for environmental factors, heat and cold resistants of V. parahaemolyticus were higher than those of V. vulnificus, withstanding for 15 minutes at 6$0^{\circ}C$ and 6 days at -18$^{\circ}C$. The pH range for existence of V. parahaemolyticus and V. vulnificus were 4.5~l1.0 and 4.5~10.0, showing the similar resistance to pH. V. parahaemolyticus and V. vulnificus could grow in media containing up to 10.0% and 7.0% NaCl, respectively, Salt-tolerance of V. parahaemolyticus was higher than that of V. vulnificus. In an antibiotics sensitivity test against 16 strains of V. parahaemolyticus, twelve strains were resistant to ampicillin, eight strains were resistant to cephalothin. one strain was resistant to streptomycin, and one strain was resistant to ticarcillin.

키워드

참고문헌

  1. Kcabra, J. J. : Fatty acids and derivatives as antimicrobial agents. Antimicrob. Agents Chemother., 2, 19-25, 1972
  2. Krieg, N. R. and Holt, J. G. : Bergey's manual of sys-tematic bacteriology. Baltimore, Williams & Wilkins, 1984
  3. Bauman, P. : Re-evaluation of taxonomy of Vibrio. Curr. Microbiol., 4, 127-132, 1980 https://doi.org/10.1007/BF02602814
  4. 국립보건원 : 감염병실험실진단지침. 국립보건원,1996
  5. 국립보건원 : 2001년 발생한 콜레라 유형의 역학적 특성(전국). 감염병발생정보,12(10), 119-121, 2001
  6. Blake, P. A.,Weaver, R. E. and Hollis,D. G. : Disease of human (other then Cholera) caused by Vibrios. Annual Review of Microbiolgy, 34, 341-367, 1984
  7. 吉田登 : Vibrio vulnificus よつて 急死し 1部檢例. 傳 染病學雜誌,64(2),236-241, 1990
  8. 김영만 : 어패류의 비브리오패혈증균 오염과 그 대책,한국식품위생학회지,8(2), 13-21, 1993
  9. 국립보건원 : 감염병실험실진단지침. 국립보건원,1996
  10. 주진우,박민정,허문수,정초록 : 부산과 대천 해안에서 V. vulnificus 와 V. parahaemolyticus의 분리 및 동정. 대한미생물학회지,35(4),309-316,2000
  11. Martinez-Manzanares : Relationship between indicators of fecal pollution in shellfish- growing water and occurrence of human pathogenic microorganisms in shellfish. J. Food Prot., 55, 609-614, 1992 https://doi.org/10.4315/0362-028X-55.8.609
  12. 주진우 : 한국 남해안 일대의 해수,해니,및 각종 해산물에서 장염비브리오균의 분리 연구. 대한미생물학회지,18(1), 1-9, 1983
  13. 백성덕,서민호,김성광 : 해수,어패류 및 생활하수에서의 Vibrio균속의 분리 동정과 항생제 감수성. 계명대학교논문집, 9(3), 312-321, 1990
  14. 손진창,정광현,허 완,권오근,박승우 : 경북 동해연안의 해수,하수 및 어패류에서 Vibrio속의 분리와 분포에 관한 조사. 경북보건환경연구원보,13, 29-46, 2000
  15. 주진우 : 한국 흑산도와 홍도해안의 Vibrio species 분리. 대한미생물학회지,25(2), 107-114, 1990
  16. Todd, E. C. D. : Preliminary estimates of costs of foodborne disease in the United States. J. Food. Prot., 52, 595-601, 1989 https://doi.org/10.4315/0362-028X-52.8.595
  17. 김순천,김개환,조성완,박종태,이 향,전두영,하동룡 : 전남해안지역에서의 Vibrio spp. 분포와 생태. 전남보건환경연구원보,2,7-17, 1990
  18. 양송주,김형선,권지영,장수현,김영만 : 금강하구에 있어서 Vibrio vulnificus의 생태학적 특성. 식품위생안전성학회,10(2), 53-59, 1995
  19. 강동훈,전상수,정덕화,조성환 : 남해연안에 분포되어 있는 Vibrio parahaemoyticus의 성상 및 Grapefruit Seed Extract 처리에 의한 항균효과. 식품위생안전성학회,9(3), 141-149, 1994
  20. 이인숙,이정복,박용배,강정복,이명진 : 경기도 연안의 장염비브리오 분포조사 및 각종 환경이 장염비브리오균 증식에 미치는 영향 조사 연구. 경기도보건환경연구소보,3, 55-70, 1989
  21. 월간첨단환경기술 : 2002 환경산업총람. 서울,환경관리연구소,2002
  22. 김상철 : Vibrio parahaemoliyticus의 환경인자에 대한 저항성. 석사학위논문,경북대학교,1987
  23. 이상조,김미정,정중교,이도영 : 경북지역에서 분리된 Vibrio균속의 분자생물학적 연구 (2000~2001). 경북보건환경연구원보,14, 9-47, 2001