DOI QR코드

DOI QR Code

Effects of Green Tea, Cocoon Hydrolysates and Capsulated Components Mixture on Body Fat and Serum Lipid Composition in College Women in Terms of Supplemented Periods

녹차와 실크 펩타이드 및 캡슐 소재 함유 조성물이 여대생들의 복용 기간별 체지방 및 혈청 지질 성상 변화에 미치는 영향

  • Lee, Sung-Hee (Aminogen Co., Ltd., Cancer Research Institute, College of Medicine, Seoul National University) ;
  • Cho, Byung-Nam (Aminogen Co., Ltd., Cancer Research Institute, College of Medicine, Seoul National University) ;
  • Hong, I-Jin (Aminogen Co., Ltd., Cancer Research Institute, College of Medicine, Seoul National University) ;
  • Lee, Min-Sook (Aminogen Co., Ltd., Cancer Research Institute, College of Medicine, Seoul National University) ;
  • Jew, Sang-Sup (Dept. of Pharmacy, Seoul National University) ;
  • Kim, Dong-Myung (Aminogen Co., Ltd., Cancer Research Institute, College of Medicine, Seoul National University) ;
  • Jin, Dong-Kyu (Anyang Hospital) ;
  • Rho, Sook-Nyung (Dept. of Food Science and Technology, Chung Ang University)
  • 이성희 ((주)아미노젠 중앙연구소) ;
  • 조병남 ((주)아미노젠 중앙연구소) ;
  • 홍이진 ((주)아미노젠 중앙연구소) ;
  • 이민숙 ((주)아미노젠 중앙연구소) ;
  • 주상섭 (서울대학교 약학대학) ;
  • 김동명 ((주)아미노젠 중앙연구소) ;
  • 진동규 (효산의료재단) ;
  • 노숙령 (중앙대학교 생활과학대학)
  • Published : 2003.07.01

Abstract

This study is to investigate effects of green tea and capsulated components mixture on body fat and serum lipid composition and fat distribution in college women students in terms of supplemented periods. During 3 months of this research, 34 college women students (average age 20.3 yr) were selected as subjects. Nutrient intake was investigated by questionnaire and 24-hr recall method. Anthropolatric assessments of the subjects were investigated by SBIA method (Segmental Bioimpedance Assay, Inbody 3.0). The results were as follows: mean body height was 162.5cm and mean body weight and mean BMI (Body Mass Index, kg/$m^2$) were 57.9kg and 21.9, respectively. Status of carbohydrate intake decreased significantly (p<0.05) and dietary fiber intake increased significantly (p<0.001) after supplementation. WHR (waist-hip ratio) decreased significantly (p<0.05) and body fat and percent body fat decreased significantly after supplementation (p<0.05). Total cholesterol and triglyceride decreased significantly after supplementation (p<0.001). Above results of this study show that green tea, silk peptide and dietary fiber supplementation-added routine diet improves body fat distribution, total cholesterol, triglyceride. Especially, declination of abdominal fat and WHR was notable, since that means diminution of risk factors for obesity and chronic degenerative diseases.

본 연구에서는 산화, 변성되기 쉬운 비타민 C와 유산균을 캡슐화하여 비타민 C의 활성을 유지시킴은 물론 유산균에 보호막을 씌움으로써 불활성화를 방지하고자 하였다. 녹차잎과 캡슐화한 비타민 C, 유산균 및 실크 펩타이드, 다시마, 차전자피, 알로에 등의 섭취가 체지방 감소 및 체중 감량에 미치는 효과를 확인하고자 여대생 34명을 대상으로 2002년 8월부터 2002년 11월까지 3개월 동안 실시하였으며, 그 결과를 요약하면 다음과 같다. 실험군의 평균 연령은 20.3세, 평균신장은 162.5 cm, 평균 체중은 57.9 kg, BMI 평균은 21.9 였다. 총열량 섭취량은 보충 전.후에 유의적인 차이는 나타나지 않았으며, 당질 섭취량은 보충 전보다 보충 후에 유의적으로 감소하는 경향으로 나타났으며 (p<0.05), 단백질과 지방의 섭취는 보충 전후에 따른 유의적인 차이는 나타나지 않았다. 식이 섬유소 섭취의 경우 보충 전에 비해 보충 후에 유의적으로 증가한 것으로 나타났다(p<0.001). 각 주별 체중 및 BMI의 변화는 유의적인 차이는 나타나지 않았으나 보충 전에 비하여 보충 후에 2.5 kg 정도 감소하는 것으로 나타났다. 체지방량 변화는 보충 전에 비해 보충 4주군에서 유의적인 감소가 나타나(p<0.05) 보충 전에 비해 보충 4주 후 2.0 kg정도 감소하는 것으로 나타났으며, 체지방율 변화 역시 보충 전보다 보충 4주 후에 유의적인 감소 경향이 나타나(P<0.05) 보충 전에 비해 보충 후 1.9%의 체지방율 감소가 나타났다. 허리-엉덩이 둘레 비율(WHR)의 변화는 보충 전에 비해 보충 4주군에서 유의한 감소 경향이 나타났다(p<0.05). 보충 전.후의 총 콜레스테롤과 중성지방의 변화는 보충 후에 유의적으로 감소하는 것으로 나타났으며 (p<0.001), LDL-cholesterol, HDL,-cholesterol 및 LDL/HDL ratio는 보충 전.후에 따른 유의적인 차이는 나타나지 않았다. 이상의 결과를 토대로 보면 본 연구에서 한달 동안 일상식이를 섭취하면서 녹차와 실크 펩타이드와 식이 섬유소의 보충은 체지방을 낮추는 역할을 하였으며, 총 콜레스테롤과 중성 지방 수준의 감소 효과를 알 수 있었다. 또한 산화, 변성되기 쉬운 비타민 C와 유산균을 캡슐화하여 비타민 C의 항산화 기능으로 혈청 지질 개선에 유의적이었으며, 정장 작용을 도움으로써 체중 감소에 효과적인 것으로 사료된다. 특히 신체의 부위 중 복부의 지방을 감소시켜 복부 비만의 관리 및 체지방 관련 위험율을 감소시키는 결과가 나타났다.

Keywords

References

  1. Kim YS. 1990. Classification and estimation of obesity. Korean J Nutrition 23: 337-340
  2. Moon HN, Hong SJ, Suh SJ. 1992. The prevalence of obesityin children and adolescents. Korean J Nutrition 25: 413-418
  3. Lim KS, Yoon YY, Kim CI, Kim KT, Kim CY, Mo SM, Choi GM. 1993. Eating behavior, obesity and serum lipid levelsin children. Korean J Nutrition 26: 56-66
  4. Huh KB. 1990. Cause of obesity. Korean J Nutrition 23:333-336
  5. Ebestein LH, Wing RR, Valoski A. 1995. Childhood obesity. Pediatr Clin North Am 32: 363-379
  6. Lee HK. 1990. Diseases related to obesity. Korean J Nutrition 23: 341-346
  7. Ashley FW Jr, Kannel WB. 1974. Relation of weight changeto changes in atherogenic traits: The Framingham study J Chronic Dis 27: 103-114 https://doi.org/10.1016/0021-9681(74)90079-4
  8. Schneeman BO. 1987. Soluble vs insoluble fiber-differentphysiological responses. Food Technol 41: 81-82
  9. Englyst HN, Cummings JH. 1985. Digestion of Polysac-charides of some cereal foods in human small intestine. Am J Clin Nutr 42: 778-787
  10. Muir JG, O'Dea K. 1992. Measurement of resistant starch:Factors affecting the amount of starch escaping digestionin vitro. Am J Clin Nutr 56: 123-127
  11. Castelli WP, Wilson PW, Levy D, Anderson K. 1990. Serum lipids and risk of coronary artery diseases. Atheroscl Rev21: 222-229
  12. Van Itallile TB. 1978. Dietary fiber and obesity. Am J Clin Nutr 31: 123-128
  13. Park JS, Shin MK, Sohn HS, Park R. 2002. Green tea (-)EGCG induces the apoptotic death of lung cancer cellsvia activation of c-Jun N-terminal kinase 1 and activatingprotein-1. Kor Nutr Soc 35: 53-59
  14. Feyes AL, Nieminen R, Ahmad ND. 1997. Green tea Con stituent epigallocatechin-3-gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells. Journal of the National of the Cancer Institute 89: 1881-1886 https://doi.org/10.1093/jnci/89.24.1881
  15. Dulloo AG, Duret C, Rohrer D. 1999. Efficacy of a green tea extract rich in catechin polyphenols and caffeine in increasing 24-h energy expenditure and fat oxidation in humans.Am J Clin Nutr 70: 1040-1045
  16. Watanabe J, Kawabata J, Niki R. 1998. Isolation and iden tification of acetyl-CoA carboxylase inhibitors from greentea (Camellia sinensis). Biosci Biotechnol Biochem 62: 532-534 https://doi.org/10.1271/bbb.62.532
  17. Lee SH, Cho BN, Hyun CK, Jew SS. 2002. Physiological, functional characteristics of silk peptide: Antioxidant effectand immune function. Food Science and Industry 35: 57-62
  18. Havel PJ, Uriu Hare JY, Lui T, Stanhope KL, Stern JS, Keen CL, Ahren B. 1998. Marked and rapid decreases of circulating leptin in streptozotocin diabetic rats: reversal byinsulin. Am J Physiol 274: 1482-1491
  19. Ahren B, Mansson S, Gingerich RL, Havel PJ. 1997. Regulation of plasma leptin in mice: influence of age, high-fatdiet, and fasting. Am J Physiol 273: 113-120
  20. Park KJ, Hong SY, Do MS, Hyun CK. 2002. Stimulation of insulin secretion by silk fibroin hydrolysate in strepto zotocin: induced diabetic rats and db/db mice. Kor J Pharmacogn 33: 21-28
  21. Lee SH, Lee CJ, Choi YJ, Ko KH. 2001. Components and antitumor activities of silk amino acid. Abstract presentedat 2001 Annual Meeting of the Korean Nutrition Society. p 85
  22. Lee SH, Lee CJ, Choi YJ. 2001. Antitumor activites and thelowering effect on the serum cholesterol level of naturalsilk hydrolysates. Abstract P8-58 presented at 67th AnnualMeeting of the Korean Society of Food Science and Technology
  23. Gould BS, Woessner JF. 1957. Biosynthesis of collagen. Theinfluence of ascorbic acid on the proline, hydroxyproline,glycine and collagen content of regenerating guinea pigskin. J Biol Chem 266: 289-300
  24. Cameron E, Pauling L. 1976. Supplemental ascorbate in thesupportive treatment of cancer: prolongation of survivaltimes in terminal human cancer. Proceedings of the Nation Academy of Sciences 73: 3685-3689 https://doi.org/10.1073/pnas.73.10.3685
  25. Kim HJ. 1989. Determination of total vitamin C by ionexclusion chromatography with electrochemical detection.J Assoc Off Anal Chem 72: 681-686
  26. Marteau P, Pochart P, Flourie B. 1990. Effect of chronicingestion of a fermented dairy Product containing Lactoba-cillus acidophilus and Bifidobacterium bifidum on metabolicactivities of the colonic flora in humans. Am J Chem Nutr52: 685-688
  27. Colombel JF, Cortot A, Neut C, Romond C. 1987. Yoghurtwith Bifidobacterium longum reduces erythromycin inducedgastrointestinal effect. Lancet 2: 43-48
  28. Clark PA, Martin JH. 1993. Selection of Bifidobacteria foruse dietary adjuncts in cultured dairy food: Tolerance tosimulated pH of human stomachs. Cult Dairy Prod J 11-14
  29. Lee KW, Kim CH, Baick SC. 1996. Utilization of milk fatfor the measurement of micro encapsulation yield. Bulletinof Dairy Food Research 7: 9-10
  30. Korea Foods Industry Association. 2001. Food Code
  31. Kim MK, Lee KH, Lim HJ, Lee SJ, Lee SH, Min KS. 1996. Preparation protocols for the functional polypeptide materials from cocoon. Korean Patent 098, 712
  32. Lee JK. 1997. Process for the preparation of yoghurt addedcapsulated lactic acid bacteria. Korean Patent 052,653
  33. The Korean Nutrition Society. 2000. Recommended dietaryallowances for Koreans. 7th ed. Chungang Publisher, Seoul.p 24-25
  34. Friedewald WT, Levy RL, Fredrickson DS. 1972. Estimationof the concentration of low density lipoprotein cholesterolin plasma, without use of the preparative ultracentrifuge,clinical chemistry, Clini Chem 18: 499-502
  35. Wallach J. 1998. Interpretation of diagnostic test. 6th ed.Little Brown and Company, Boston. p 477-485
  36. Lee YH, Lee HY, Noh SR, Ahn SJ, Lee BH. 1997. Healthand Nutrition. Chungang university Publisher, Korea. p 95-109
  37. Polloick ML, Jacson AS. 1994. Research progress in Validation of clinical methods of assessing body composition. Med. Sci Sports Exerc 16: 606-613
  38. Sayama K, Lin S, Zheng G, Oguni I. 2000. Effects of greentea on growth, food utilization and lipid metabolism in mice.In Vivo 14: 481-484
  39. Kim MJ, Lee SS. 1995. The effect of dietary fiber on theserum lipid level and bowel function in rats. Korean J Nutrition 28: 23-32
  40. Jeong KA. 1995. Effect of cereals on lipid concentration ofliver and serum in the rats. Korean J Nutrition 28: 11-13

Cited by

  1. Influences of Chitosan, Sericin and Collagen Extract Complexes on the Improvement Actions of Lipid Component in Diabetes vol.20, pp.8, 2011, https://doi.org/10.5322/JES.2011.20.8.1031
  2. Influences of Silkworm Sericin on the Improvement Actions of Lipid Metabolism in Dyslipidemic Rats vol.20, pp.10, 2010, https://doi.org/10.5352/JLS.2010.20.10.1525
  3. Improvements Caused by Chitosan, Sericin and Collagen Peptide Extract Complexes on Lipid Metabolism in Dyslipidemia vol.20, pp.8, 2011, https://doi.org/10.5322/JES.2011.20.8.1021
  4. Introduction to the Technology, Applications, Products, Markets, R&D, and Perspectives of Nanofoods in the Food Industry vol.11, pp.4, 2003, https://doi.org/10.3746/jfn.2006.11.4.348