The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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A Subpopulation of RNA3 of Cucumber mosaic virus Quasispecies

  • Park, Seung-Kook (Department of Biochemistry and Biophysics, Texas A&M University) ;
  • Park, Sun-Hee (Plant Virus GenBank, PVGABC, Division of Life and Environmental Sciences, Seoul Women's University) ;
  • Yoon, Ju-Yeon (Plant Virus GenBank, PVGABC, Division of Life and Environmental Sciences, Seoul Women's University) ;
  • Park, Jang-Kyung (Division of Biological Environment, Kangwon National University) ;
  • Ryu, Ki-Hyun (Plant Virus GenBank, PVGABC, Division of Life and Environmental Sciences, Seoul Women's University)
  • Published : 2003.08.01
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Abstract

This study examined the existence of genetically diverse population of Cucumber mosaic virus (CMV), known as quasispecies, from lily, Nicotiana benthamiana and from purified virions. Based on the conserved sequences of CMV lily isolates in intergenic region (IR) on RNA3, the genetic variation of IR from three different sources was investigated by a specific restriction endonuclease hydrolysis of amplified reverse transcription-polymerase chain reaction (RT-PCR) products using virus-specific primers, and was compared with IR sequences. The IR nucleotide sequences of CMV lily isolates were highly conserved, however, quasispecies was detected from all three sources in low level, containing sub-populations of RNA3. These subpopulations of RNA3 were inoculated onto zucchini squash by in vitro transcripts from corresponding full-length cDNA clones together with Eny RNA1 and 2 transcripts. The systemic symptom of zucchini plants infected by these quasispecies was chlorotic spotting, which was milder than severe mosaic and stunt symptom caused by Eny-CMV. The severity of symptom was correlated with RNA accumulation of viruses. These results suggest that the genome of CMV lily isolates consists of quasispecies populations.

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References

  1. Aranda, M.A., Fraile, A and Garcia-Arenal, F. 1993. Genetic variability and evolution of the satellite RNA of cucumber mosaic virus during natural epidemics. J. Virol. 67:5896-5901
  2. Boccard, F. and Baulcombe, D. 1993. Mutational analysis of cisacting sequences and gene function in RNA3 of cucumber mosaic virus. Virology 93:563-78
  3. Canto, T., Proir, T.A., Hellwald, K.H., Oparka, K. and Palukaitis, P. 1997. Characterization of cucumber mosaic virus. IV. Movement protein and coat protein are both essential for cell-to-cell movement of cucumber mosaic virus. Virology 237:237-248 https://doi.org/10.1006/viro.1997.8804
  4. Canto, T., Choi, S.K and Palukaitis, P. 2001. A subpopulation of cucumber mosaic virus RNA1 contains 3' termini originating from RNAs 2 or 3. J. Gen. Virol. 82:941-945 https://doi.org/10.1099/0022-1317-82-4-941
  5. Carrere, I., Tepfer, M. and Jacquemond, M. 1999. Recombinants of cucumber mosaic virus (CMV): determinants of host range and symptomatology. Arch. Virol. 144:365-379 https://doi.org/10.1007/s007050050510
  6. Chen, Y.K. Derks, A.F.L.M., Langeveld, S., Goldbach, R. and Prins, M. 2001. High sequence conservation among cucumber mosaic virus isolates from lily. Arch. Virol. 146:1631-1636 https://doi.org/10.1007/s007050170085
  7. Chen, Y.K., Goldbach, R. and Prins, M. 2002. Inter- and intramolecular recombinations in the cucumber mosaic virus genome related to adaptation to alstroemeria. J. Virol. 76:4119-4124 https://doi.org/10.1128/JVI.76.8.4119-4124.2002
  8. Domingo, E., Escarrnis, C., Sevilla, N., Moya, A., Elena, S.F., Quer, J., Novella, I. and Holland, J.J. 1996. Basic concepts in RNA virus evolution. FASEB J. 10:859-864
  9. Domingo, E., Ruiz-Jarabo, C.M., Sierra, S., Arias, A, Pariente, N., Baranowski, E. and Escarrnis, C. 2002. Emergence and selection of RNA virus variants: memory and extinction. Virus Res. 82:39-44
  10. Gal-On, A., Kaplan, I., Roossinck, M. and Palukaitis, P. 1994. The kinetics of infection of zucchini squash by cucumber mosaic virus indicates a function RNA1 in virus movement. Virology 205:280-289 https://doi.org/10.1006/viro.1994.1644
  11. Gal-On, A., Kaplan, I., Roossinck, M. and Palukaitis, P. 1995. Differential effects of satellite RNA on the accumulation of cucumber mosaic virus RNAs and their encoded proteins in tobacco vs. zucchini squash with two strains of CMV helper virus. Virology 208:58-66 https://doi.org/10.1006/viro.1995.1129
  12. Hayes, R.J. and Buck, K.W. 1990. Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363-368 https://doi.org/10.1016/0092-8674(90)90169-F
  13. Jung, H.J., Veda, S., Ryu, K.H., Lee, S.Y. and Choi, J.K. 2000. A novel strain of cucumber mosaic virus isolated from Lilium longiflorum. Plant Pathol. J. 16:306-311
  14. Kaplan, I.E., Gal-On, A and Palukaitis, P. 1997. Characterization of cucumber mosaic virus: III. Localization of sequence in the movement protein controlling systemic infection in cucurbits. Virology 230:343-349 https://doi.org/10.1006/viro.1997.8468
  15. Kurath, G. and Palukaitis, P. 1989. RNA sequence heterogeneity in natural populations of three satellite RNAs of cucumber mosaic virus. Virology 173:231-240 https://doi.org/10.1016/0042-6822(89)90239-0
  16. Liu, S., He, X., Park, G., Josefsson, C. and Perry, K.L. 2002. A conserved capsid protein surface domain of cucumber mosaic virus is essential for efficient aphid vector transmission. J. Virol. 76:9756-9762 https://doi.org/10.1128/JVI.76.19.9756-9762.2002
  17. Masuta, C., Seshimo, Y., Mukohara, M., Jung, H.J., Veda, S., Ryu, K.H. and Choi, J.K. 2002. Evolutionary characterization of two lily isolates of Cucumber mosaic virus isolated in Japan and Korea. J. Gen. Plant Pathol. 68:163-168 https://doi.org/10.1007/PL00013070
  18. Palukaitis, P. and Roossinck, M.J. 1995. Variation in the hypervariable region of cucumber mosaic virus satellite RNAs is affected by the helper virus and the initial sequence context. Virology 206:765-768 https://doi.org/10.1016/S0042-6822(95)80006-9
  19. Palukaitis, P., Roossinck, M.J., Dietzgen, R.G. and Francki, R.I.B.1992. Cucumber mosaic virus. Adv. Virus Res. 41:281-348 https://doi.org/10.1016/S0065-3527(08)60039-1
  20. Peden, K.W.C. and Symons, R.H. 1973. Cucumber mosaic virus contains a functionally divided genome. Virology 53:487-492 https://doi.org/10.1016/0042-6822(73)90232-8
  21. Rizzo, T.M. and Palukaitis, P. 1990. Construction of full-length cDNA clones of cucumber mosaic virus RNAs 1. 2. and 3:generation of infectious RNA transcripts. Mol. Gen. Genet. 222:249-256 https://doi.org/10.1007/BF00633825
  22. Rodriguez-Alvarado, G., Kurath, G. and Dodds, A. 1995. Heterogeneity in pepper isolates of cucumber mosaic virus. Plant Dis. 79:450-45524 https://doi.org/10.1094/PD-79-0450
  23. Roossinck, M.J. and Palukaitis, P. 1990. Rapid induction and severity of symptoms in zucchini squash (Cucurbita pepo) map to RNA1 of cucumber mosaic virus. Mol. Plant-Microbe Interat. 3:188-192 https://doi.org/10.1094/MPMI-3-188
  24. Roossinck, M.J. and Palukaitis, P. 1991. Differential replication in zucchini squash of a cucumber mosaic virus satellite RNA maps to RNA 1 of the helper virus. Virology 181:371-373 https://doi.org/10.1016/0042-6822(91)90506-7
  25. Roossinck, M.J. 1997. Mechanism of plant virus evolution. Annu. Rev. Phytopathol. 35: 191-209 https://doi.org/10.1146/annurev.phyto.35.1.191
  26. Ryu, K.H., Park, H.W. and Choi, J.K. 2002. Characterization and sequence analysis of a lily isolate of cucumber mosaic virus from Lilium tsingtauense. Plant Pathol. J. 18:85-92 https://doi.org/10.5423/PPJ.2002.18.2.085
  27. Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York
  28. Sanger, F., Nicklen, S. and Coulson, A.R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467 https://doi.org/10.1073/pnas.74.12.5463
  29. Schneider, W.L., Roossinck, M.J. 2000. Evolutionarily related Sindbis-like plant viruses maintain different levels of population diversity in a common host. J. Virol. 74:3130-3134 https://doi.org/10.1128/JVI.74.7.3130-3134.2000
  30. Schneider, W.L. and Roossinck, M.J. 2001. Genetic diversity in RNA virus quasispecies is controlled by host-virus interactions. J. Virol. 75:6566-6571 https://doi.org/10.1128/JVI.75.14.6566-6571.2001