The Transactions of the Korean Institute of Electrical Engineers C (대한전기학회논문지:전기물성ㆍ응용부문C)

The Korean Institute of Electrical Engineers (대한전기학회)
권호 표지

Development of New DNA Chip and Genome Detection Using an Indicator-free Target DNA

비수식화 DNA를 이용한 유전자 검출 및 새로운 DNA칩의 개발

  • Park, Yong-Sung (Dept.of Electric Electronics Information Engineering, Wonkwang University) ;
  • Park, Dae-Hee (Dept.of Electric Electronics Information Engineering, Wonkwang University) ;
  • Kwon, Young-Soo (Dept.of Electric Electronics Computer Engineering, Donga University) ;
  • Tomoji Kawai (Research Institute of Industrial Science, Osaka University)
  • Published : 2003.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

This research aims to develop an indicator-free DNA chip using micro-fabrication technology. At first, we fabricated a DNA microarray by lithography technology. Several probe DNAs consisting of thiol group at their 5-end were immobilized on the gold electrodes. Then indicator-free target DNA was hybridized by an electrical force and measured electrochemically in potassium ferricyanide solution. Redox peak of cyclic-voltammogram showed a difference between target DNA and mismatched DNA in an anodic peak current. Therefore, it is able to detect various genes electrochemically after immobilization of various probe DNAs and hybridization of indicator-free DNA on the electrodes simultaneously It suggested that this DNA chip could recognize the sequence specific genes.

Keywords

References

  1. M. J. O'Donnell, K. Tang, H. Koster, C. L. Smith, and C. R. Cantor, 'High-Density, Covalent Attachment of DNA to Silicon Wafers for Analysis by MALDI-TOF Mass Spectrometry', Anal Chem, Vol. 69, pp. 2438-2443, 1997 https://doi.org/10.1021/ac961007v
  2. E. M. Southern, S. C. Case-Green, J. K. Elder, M. Johnson, K. U. Mir, L. Wang and J. C. Williams, 'Arrays of complementary oligonucleotides for analysing the hybridisation behaviour of nucleic acids', Nucleic Acids Res., Vol. 22, pp. 1368-1373, 1994 https://doi.org/10.1093/nar/22.8.1368
  3. A. C. Pease, D. Solas, E. J. Sullivan, M. T. Cronin, C. P. Holmes and S. P. A. Fodor, 'Light-Generated Oligonucleotide Arrays for Rapid DNA Sequence Analysis', Proc. Natl. Acad, of Sci. U.S.A., Vol. 91, pp. 5022-5026, 1994 https://doi.org/10.1073/pnas.91.11.5022
  4. A. B. Steel, T. M. Herne, and M. J. Tarlov, 'Electrochemical Quantitation of DNA Immobilized on Gold', Anal. Chem, Vol. 70, pp. 4670-4677, 1998 https://doi.org/10.1021/ac980037q
  5. R. F. Service, 'Microchip Arrays Put DNA on the Spot', Science, Vol. 282, pp. 396-399, 1998 https://doi.org/10.1126/science.282.5388.396
  6. M. Schena, D. Shalon, R. W. Davis, P. O. Brown, 'Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray', Science, Vol. 270, pp. 467-470, 1995 https://doi.org/10.1126/science.270.5235.467
  7. D. Shalon, S. J. Smith, and P. O. Brown, 'A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization', Genome Res.. Vol. 6, pp. 639-645, 1996 https://doi.org/10.1101/gr.6.7.639
  8. J. R. Pollack, C. M. Perou, A. A. Alizadeh, M. B. Eisen, A. Pergamenschikov, C. F. Williams, S. S. Jeffrey, D. Botstein, P. O. Brown, 'Genome-wide analysis of DNA copy-number changes using cDNA microarrays', Nat. Genet, Vol. 23(1), pp. 41-46, 1999 https://doi.org/10.1038/12640
  9. E. Palecek, M. Fojta, M. Tomschik and J. Wang, 'Electrochemical biosensors for DNA hybridization and DNA damage' Biosens. Bioelectron, Vol. 13, pp. 621-628, 1998 https://doi.org/10.1016/S0956-5663(98)00017-7
  10. D. H. Johnston, K. C. Glasgow, and H. H. Thorp, 'Electrochemical Measurement of the Solvent Accessibility of Nucleobases Using Electron Transfer between DNA and Metal Complexes', J. Am Chem Soc., Vol. 117, pp. 8933-8938, 1995 https://doi.org/10.1021/ja00140a006
  11. S. O. Kelly, E. M. Boon, J. K. Barton, N. M. Kackson and M. G. Hill, 'Single-base mismatch detection based on charge transduction through DNA', Nucleic. Acids Res., Vol. 27, pp. 4830-4837, 1999 https://doi.org/10.1093/nar/27.24.4830
  12. M. I. Pividori, A. MerkoCi and S. Alegret, 'Electrochemical genosensor design: immobilisation of oligonucleotides onto transducer surfaces and detection methods', Biosens. Bioelectron., Vol. 15, pp. 291-303, 2000 https://doi.org/10.1016/S0956-5663(00)00071-3
  13. J. Wang, G. Rivas, J. R. Femandes, J. L. L. Paz, M. Jiang, R. Waymire, 'Indicator-free electrochemical DNA hybridization biosensor', Anal Chim Acta, Vol. 375, pp. 197-203, 1998 https://doi.org/10.1016/S0003-2670(98)00503-0