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Effects of Liquid Culture of Agaricus blazei Murill on Growth, Lipid and Protein Levels, and Enzyme Activities in Rats

신령버섯 균사체 배양액이 흰쥐의 성장률, 지질과 단백질 농도 및 효소활성에 미치는 영향

  • 고진복 (신라대학교 생명과학과)
  • Published : 2003.08.01

Abstract

The effects of liquid culture of Agaricus blazei Murill on the body weight gain, lipid metabolism, protein levels, and enzyme activities were studied in growing male rats. Sprague-Dawley rats were divided into three groups ; control group (A. blazei free water), 20 or 30% A. blazei groups (20 or 30% A. blazei in water) according to the levels of A. blazei supplementation. The rats were fed ad libitum each of the experimental beverage for 5 weeks. The body weight gain, food intake, food efficiency ratio, and organ weight of the 20 or 30% A. blazei groups were similar to those of the control group. There were no differences in the concentrations of triglyceride, total cholesterol, HDL-cholesterol, total protein and albumin, and the activities of GOT, GPT, LDH and alkaline phosphatase of the serum among the experimental groups. The calcium and phosphorus concentrations of serum in the 20 or 30% A. blazei group were significantly decreased compared with those of the control group. The concentrations of LDL-cholesterol and the atherogenic index of the 20 or 30% A. blazei groups were significantly lower than those of the control group, but the serum HDL-cholesterol/total cholesterol ratio was higher. These results showed that the 20 or 30% A. blazei feeding decreased the LDL-cholesterol and the atherogenic index, and increased HDL-cholesterol/total cholesterol ratio in serum of rats.

신령버섯의 균사체 배양액이 성장기 흰쥐에 미치는 영향을 관찰하고자 생후 7주령의 흰쥐 수컷을 대상으로 음료수에 20% 및 30% 수준으로 신령버섯 균사체 배양액을 혼합하여 5주간 급여하고 성장률, 식이섭취량, 식이효율, 장기의 무게, 간과 혈청의 지질 농도, 혈청의 단백질 농도 및 효소 활성을 조사한 결과는 다음과 같다. 체중증가량, 식이섭취랑, 식이효율 및 장기의 무게는 대조군과 신령버섯 음료군들이 유의한 차이를 보이 지 않았다. 간과 혈청 의 중성 지질과 콜레스테롤 농도 및 혈청의 HDL-콜레스테를 농도는 대조군과 신령버섯 음료군들이 비슷한 수준으로 나타났다. 혈청의 LDL-콜레스테를 농도 및 동맥경화지수는 대조군에 비해 신령버섯 음료군들이 유의하게 감소되었다. 혈청의 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율은 대조군에 비해 신령버섯 음료군들이 유의하게 증가되었다. 혈청의 총 단백질, 알부민, 혈당, 혈색소, 마그네슘 등의 농도는 각 수준별 신령버섯 음료군이 대조군과 비슷한 농도로 신령버섯 음료 섭취에 따른 영향은 나타나지 않았다. 혈청의 GPT, GOT, LDH및 ALP 등의 활성도 신령버섯 음료 섭취에 따른 영향은 나타나지 않았다. 혈청의 칼슘과 인의 농도는 대조군에 비해 신령버섯 음료군들이 유의하게 감소되었다. 이상의 결과로보아 성장기 흰쥐에 신령버섯 균사체 배양액을 20% 및 30% 수준으로 급여시 성장률, 간과 혈청의 콜레스테를 및 중성지질 농도, 단백질 농도 및 효소 활성은 정상수준을 유지하였고, 혈청의 LDL 콜레스테를 농도 및 동맥경화지수를 낮추는 효과가 있고, 혈청의 총 콜레스테롤에 대한 HDL-콜레스테롤의 비율을 높여주는 효과가 있는 것으로 나타났다.

Keywords

References

  1. Kawagish H, Kasumi R, Sazawa T, Mizuno T, Hagiwara T, Nakamura T. 1988. Cytotoxic steroids from the mushroom Agaricus blazei. Phytchemistry 27: 2777-2779. https://doi.org/10.1016/0031-9422(88)80662-9
  2. Takaku T, Kimura Y, Okuda J. 2001. Isolation of an antitumor compound from Agaricus blazei Muril and its mechanism of action. J Nutr 131: 1409-1413.
  3. Chang HL, Chao GR, Chen CC, Mau JL. 2001. Non-volatile taste components of Agaricus blazei, Antrodia camphorata and Cordyceps militaris mycelia. Food Chemistry 74: 203-207. https://doi.org/10.1016/S0308-8146(01)00127-3
  4. Dong Q, Yao J, Yang XT, Fang JN. 2002. Structural characterization of af water-soluble $\beta$-D-glucan from fruiting bodies of Agaricus blazei Murr. Carbohydrate Research 337: 1417-1421. https://doi.org/10.1016/S0008-6215(02)00166-0
  5. Kawagish H, Inagaki R, Kanao T. 1989. Fraction and antitumor activity of the water-insoluble residue of Agaricus blazei fruiting bodies. Carbohydrate Research 186: 267-273 https://doi.org/10.1016/0008-6215(89)84040-6
  6. Mizuno T, Hagiwara Y, Sumiya T. 1990. Antitumor activity and some properties of water soluble polysaccharides from the fruiting body of Agaricus blazei Muril. Agric Biol Chem 54: 2889-2896. https://doi.org/10.1271/bbb1961.54.2889
  7. Mizuno T, Inagaki R, Kanao T, Hagiwara T. 1990. Antitumor activity and some properties of water-insoluble hetero-glucans from “Himematsutake” the fruiting body of Agaricus blazei Muri. Agric Biol Chem 54: 2897-2905. https://doi.org/10.1271/bbb1961.54.2897
  8. Ku HO, Kim IC, Lee YS. 1999. Resistance effects on the infection of Staphylococcus aureus and the growth of Sarcorma 180 cell by polysaccharide separated from in mice. Korean J Lab Anim Sci 15: 155-158.
  9. Itoh H, Amano H, Noda H. 1994. Inhibitory action of a ($1{\rightarrow}6$)-$\beta$-D-glucan-protein complex isolated from Agaricus blazei Muril on metha fibrosarcoma-bearing mice and its antitumor mechanism. Jpn J Pharmacol 66: 265-271. https://doi.org/10.1254/jjp.66.265
  10. Yoshiaki F, Hidekazu K, Koichi O, Ryo S, Takusaburo E. 1998. Tumoricidal activity of high molecular weight polysaccharides derived from Agaricus blazei via oral administration in the mouse tumor model. Nippon Shokuhim Kagaku Kaishi 45: 246-252. https://doi.org/10.3136/nskkk.45.246
  11. Fujimiya Y, Suzuki Y, Katakura R, Ebina T, 1999. Tumor-specific cytocidal and immunopotentiating effects of relatively low molecular weight products derived from the basidiomycete, Agaricus blazei Murill. Anticancer Res 19: 113-118.
  12. Menoli RCRN, Mantovani MS, Ribeiro LR, Speit G, Jordao BQ. 2001. Antimutagenic effects of the mushroom Agaricus blazei Murrill extracts on V79 cells. Mutation Research 496: 5-13. https://doi.org/10.1016/S1383-5718(01)00227-3
  13. Ji JH, Kim MN, Choi KP, Chung CK, Ham SS. 2000. Antimutagenic and cytotoxicity effects of Agaricus blazei Murill extracts. Korean J Food Sci Technol 32: 1371-1378.
  14. Choi JM, Koo SJ. 2000. Effects of $\beta$-glucan from Agaricus blazei Murill on blood glucose and lipid composition in db/db mice. Korean J Food Sci Technol 32: 1418-1425
  15. Sugiyama K, Saeki S, Ishiguro Y. 1992. Hypercholesterolemic activity of ningyotake (Polyporus confleuens) mushroom in rats. J Jpn Soc Nutr Food Sci 45: 265-270. https://doi.org/10.4327/jsnfs.45.265
  16. Reeves PG, Nielsen FH, Fahey GC. 1993. AIN-93 purified diets for laboratory rodents final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123: 1939-1951.
  17. Medical Laboratory. 1969. Technology and Clinical Pathology. 2th ed. WB. Saunders Co., Philadelphia. p 673.
  18. Davidson I, Henry JB. 1966. Todd-Sanford clinical diagnosis by laboratory methods. 13th ed. WB. Saunders Co., Philadelphia. p 73-75.
  19. Warnick GR, Knoopp RH, Fitzpatrick V, Branson L. 1990. Estimating low density lipoprotein cholesterol by the Friedewald equation is adequate for classifying patient on the basis of nationally recommended cutpoints. Clin Chem 36: 15-19.
  20. Schectman G, Patsches M, Sasse EA. 1996. Variability in cholesterol of measurements: comparison of calculated and direct LDL cholesterol determinations. Clin Chem 42: 732-737.
  21. Friedwald WT, Levy RI, Fedreicson DS. 1979. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499-508.
  22. Haglund O, Loustarinen R, Wallin R, Wibell I, Saldeen T. 1991. The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in humans supplemented with vitamin. Eur J Nutr 121: 165-172.
  23. Folch J, Lees M, Stanley GSH. 1957. A simple method for the isolation and purification of total lipid from animal tissues. J Biol Chem 226: 497-509.
  24. Frings CS, Dunn RT. 1970. A colorimetric method for determination of total serum lipid based on the sulfo-phospho-vanillin reaction. Am J Clin Path 53: 89-91.
  25. Koh JB. 2001. Effect of fruiting body of Cordyceps militaris on growth, lipid and protein metabolism and enzyme activities in male rats. Korean J Nutrition 34: 741-747.
  26. Koh JB. 2002. Effect of mycelium of Cordyceps militaris on growth, lipid metabolism and protein levels in male rats. J Korean Soc Food Sci Nutr 31: 65-690. https://doi.org/10.3746/jkfn.2002.31.4.685
  27. Cheung PCK. 1996. The hyporcholesterolemic effect of extracellular polysaccharide from the submerged fermentation of mushroom. Nutr Res 16: 1953-1957. https://doi.org/10.1016/S0271-5317(96)00218-7
  28. Ebihara K, Schnceman BO. 1989. Interaction of bile acids, phospholipids, cholesterol and triglycerides with dietary fiber inthe small intestine of rats. J Nutr 119: 1100-1106.
  29. Kinosian B, Glick H, Preiss L, Puder KI. 1995. Cholesterol and coronary heart disease: predicting risk in men by changes in levels and ratios. J Invest Med 43: 443-450.
  30. Kailash P. 1999. Reduction of serum cholesterol and hyper-cholesterolemic atherosclerosis in rabbits by secoisolariciresinol diglucoside isolated from flaxseed. Circulation 99: 1355-1362. https://doi.org/10.1161/01.CIR.99.10.1355
  31. Kim HY, Song SW, Ha CS, Han SS. 1993. Effects of the population density on growth and various physiological values of Sprague-Dawley rats. Korean J Lab Ani Sci 9: 71-78.
  32. Kang BH, Son HY, Ha CS, Lee HS, Song SW. 1995. Reference value of hematology and serum chemistry in Ktc: Sprague-Dawley rats. Korean J Lab Ani Sci 11: 141-145.
  33. Torre M, Roddriguez AR, Saura FC. 1991. Effects of dietary fiber and phytic acid on mineral availability. Crit Rev Food Sci Nutr 1: 1-22. https://doi.org/10.1080/10408399109527539
  34. Kim JY, Koh JB. 2003. Effects of fibers and lipid levels on mineral concentrations in ethanol-treated rats. J Nat Sci Silla Univ 11: 1-11.
  35. Burtis CA, Ashwood ER. 1999. Tietz Textbook of Clinical Chemistry. 3th ed. WB. Saunders Co., Philadelphia. p 652-656.

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