DOI QR코드

DOI QR Code

Effects of Powdered Pine Needle (Pinus densiflora seib et Zucc.) on Serum and Liver Lipid Composition and Antioxidative Capacity in Rats Fed High Oxidized Fat

솔잎분말이 과산화지질을 급여한 흰쥐의 혈장 및 간장의 지질구성과 항산화능에 미치는 영향

  • Lee, Eun (Dept. of bio-industry and technology, Sangji University)
  • 이은 (상지대학교 생명산업학과)
  • Published : 2003.08.01

Abstract

Effects of powdered pine needle (Pinus densiflora seib et Zucc) on serum and liver lipid composition and antioxidative capacity were investigated in rat fed high oxidized fat. Sixty male Sprague-Dawley rats weighing 161.25$\pm$2.51 g were blocked into four groups according to body weight and raised seven weeks with basal diet (normal group, I), basal diet and 10% oxidized fat (control group, II), basal diet, 10% oxidized fat and 2% powdered pine needle (2% powdered pine needle group, III) and basal diet, 10% oxidized fat and 3% powdered pine needle (3% powdered pine needle group, IV). Food intake, body weight gain and feed efficiency were not significantly different among oxidized fat diet groups. The level of plasma total cholesterol showed a tendency to decrease, whereas the plasma HDL-cholesterol concentration revealed a tendency to increase in pine needle groups. However plasma triglyceride level showed no significant differences in the treatment groups. Intake of the oxidized fat has increased the levels of liver cholesterol and triglyceride. The powdered pine needle showed a tendency to decrease thiobarbituric acid values in plasma and liver. The pine needle samples have also decreased the plasma GOT and GPT activities, whereas they have increased the liver glutathione peroxidase activity.

솔잎분말이 과산화지질을 급여한 횐쥐의 혈장 및 간장지질구성과 항산화능에 미치는 영향을 검토했다. 일일 식이섭취량은 정상군을 비롯한 과산화지질처리군 모두에서 유의한 차이를 나타내지 않았다. 일일체중증가율 및 식이효율은 과산화지질처리군 모두가 정상군보다 높았으나, 대조군과 솔잎처리군 간에는 유의한 차이를 나타내지 않았다. 혈장 총콜레스테롤량은 과산화지질처리에 의해 증가하였으나, 솔잎 처리에 의해 감소되었다. 혈장 HDL-cholesterol량은 솔잎처리군에서 높은 경향을 나타내었다. 혈장 중성지질량은 처리군간에 유의한 차이를 나타내지 않았다. 간장의 총콜레스테를량과 중성지질량은 과산화지질처리군에서 증가하였으나, 솔잎처리에 따른 유의한 차이는 볼 수 없었다. 혈장 및 간장내 TBARS량과 혈장 GOT 및 GPT의 활성치는 과산화지질처리군에서 증가하였으나, 솔잎처리에 의해 감소되었다(p<0.05). 간장 glutathione peroxidase 활성치는 솔잎처리에 의해 증가하는 경향을 보였으나, 대조군과 유의한 차이를 나타내지는 않았다(p>0.05).

Keywords

References

  1. Bidlack WR, Tappel AL. 1973. Damage to microsomal membrane by lipid peroxidation. Lipids 8: 177-178. https://doi.org/10.1007/BF02544631
  2. Saito M. 1988. Interaction between lipid peroxide formation and nutritional status. J JPN Soc Nutr Food Sci 41: 343-349. https://doi.org/10.4327/jsnfs.41.343
  3. Vergroeson AT. 1997. Physiological effects of dietary linoleic acid. Nutr Rev 35: 1-9.
  4. Younes M, Siegers CP. 1980. Lipid peroxidation as a consequence of glutathione depletion in rat and mouse liver. Res Comm Chem Path Pharm 27: 119-129.
  5. Schwartz MK. 1980. Role of trace elements in cancer. Cancer Res Comm Chem Path Pharm 29: 319-331.
  6. Langanier S, Yu BP. 1987. Anti-lipoperoxidation action of food restriction. Biochem Biophys Res Comm 145: 1185-1202. https://doi.org/10.1016/0006-291X(87)91562-2
  7. Powell CJ, Connoly AK. 1988. The site specificity and sensitivity of the rats liver to butylated hydroxytoluene-induced damage. Toxicol Appl Pharmacoll 5: 1127-1145.
  8. Yamaoka Y, Kawakita T, Kaneko M, Nomoto K. 1996. A polysaccharide fraction of Zizyphi fructus in augmenting natural killer activity by oral administration. Biol Pharm Bull 19: 936-939. https://doi.org/10.1248/bpb.19.936
  9. Jung DW, Shibuya M, Ebizuka Y, Yoshimatsu K, Shimomura K, Chung KS. 1998. ELISA for the determination of saiko-saponin a, an active component of Bupleuri radix. Chem Pharm Bull 46: 1140-1143. https://doi.org/10.1248/cpb.46.1140
  10. Yoshikawa M, Murakami T, Hirano K, Inadzuki M, Ninomiya K, Matsuda H, Scorzonerosides AB, Novei C. 1997. Triterpene oligoglycosides with hepatoprotective effect from chines Bupleuri radix, the roots of Bupleurum scorzonerifolium willd. Telrahearon Letters 38: 7395-7398. https://doi.org/10.1016/S0040-4039(97)01733-4
  11. Park JK. 1984. Hanbang daewejeun. Dongyang jonghab tongsingyoyukweon, Daegu. p 134-136.
  12. MBC. 1988. Hankook minganyobobdaejeun. Kumbak pub. Co., Seoul. p 21-25.
  13. Choi OJ. 1991. Yeuakcho seungbun, iyong. Illweulseugak, Seoul. p 114-116.
  14. Kang YH, Park YK, Ha TY, Moon KD. 1996. Effects of pine needle extracts on serum and liver lipid contents in rat fed high fat diet. J Korean Soc Food Nutr 25: 367-373.
  15. Kang YH, Park YK, Ha TY, Moon KD. 1996. Effects of pine needle extracts on enzyme activities of serum and liver, liver morphology in rats fed high fat diet. J Korean Soc Food Nutr 25: 374-378.
  16. Choi MY, Choi EJ, Lee E, Rhim TJ, Cha BC, Park HJ. 1997. Antimicrobial activities of pine needle (Pinus densiflora seib et Zucc.). Kor J Appl Microbiol Biotechnol 25: 293-297.
  17. Buege JA, Aust SD. 1978. Microsomal lipid peroxidation. In Methods in enzymelogy. Fleischer S, Packer L, eds. Academic press, London. Vol 52, p 302-309. https://doi.org/10.1016/S0076-6879(78)52032-6
  18. Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
  19. Levander OA, DeLoach DP, Morris VC, Moser PB. 1983. Platelet glutathione peroxidase activity as an index of selenium status in rats. J Nutr 113: 55-63.
  20. Ahrens EH, Insull WJ, Blomstrand R, Hirsh J, Tsaltas TT, Peterson MI. 1957. The influence of dietary fat on serum lipids levels in man. Lancet 1: 943-954.
  21. Kim SH, Jo MJ. 1972. A study of metabolic effect in high and low fat diet on albino rat. J Nutr 5: 169-183.
  22. Kim WY, Park HS. 1981. The effect of dietary fat levels and protein source in early life on the cholesterol and lipid metabolism in adult rats. Korean J Nutr 14: 136-147.
  23. Sin MK, Han GJ. 1997. The effects of green tea on the serum lipid and liver tissue of cholesterol fed rats. Korean J Sci Technol 29: 1255-1263.
  24. Kang YH, Ha TY, Moon KD. 1996. Effects of pine needle extracts on serum and liver lipid contents in rats fed high fat diet. J Korean Soc Food Nutr 25: 367-373.
  25. Hang A, Hostmark AT. 1987. Lipoprotein lipases, lipoprotein and tissue lipids in rats fed fish oil or coconut oil. J Nutr 117: 1011-1017.
  26. Harris WS, Connor WE, McMurry MP. 1983. The comparative reductions of the plasma lipids and lipoproteins by dietary polyunsaturated fats: salmon oil versus vegetable oils. Metabolism 32: 179-184. https://doi.org/10.1016/0026-0495(83)90226-3
  27. Sanders TAB, Hochland MC. 1983. A comparison of the influence of on plasma lipids and platelet function of supplements of n-3 and polyunsaturated fatty acid. British J Nutr 50: 521-529. https://doi.org/10.1079/BJN19830123
  28. Chung YJ, Park HJ, Chang YK. 1994. Effect of dietary eicosapentaenoic acid on serum and liver lipids paterns of mail rat. Korean J Nutr 27: 537-551.
  29. Droubay PE, Puppione DL. 1980. Dietary fat-induced post-prandial lipemia: Effect on arterial oxygen saturation as plasma lactate, triglyceride and cholesterol levels in subjects with argina pectoris. Am J Clin Nutr 33: 1199-1215.
  30. Kay RM, Sabry ZI, Csima A. 1980. Multivariate analysis of diet and serum lipids in normal men. Am J Clin Nutr 33: 2566-2581.
  31. Krichevsky D, Tepper SA. 1968. Experimental atherosclerosis in rabbits fed cholesterol free diet. Influence of chow components. J Atheroscler Res 8: 357-372. https://doi.org/10.1016/S0368-1319(68)80070-5
  32. Kubo DK, Mazda DD, Tanaka SK, Kimura SK, Kei CR, You JH, Okuta TD. 1980. The study of Ganoderma luciodum. Kisho to Rinsho 14: 2455-2470.

Cited by

  1. Effects of Pine Needle Extract on Differentiation of 3T3-L1 Preadipocytes and Obesity in High-Fat Diet Fed Rats vol.29, pp.10, 2006, https://doi.org/10.1248/bpb.29.2111
  2. Biological Effects of Allium monanthum Extracts on Lipid Metabolism, Anti-oxidation and the Production of Pro-inflammatory Cytokines in Rats Fed a High-Fat Diet vol.26, pp.3, 2013, https://doi.org/10.7732/kjpr.2013.26.3.337
  3. Effects of Dietary Supplementation of Yacon(Polymnia sonchifolia) By-products and Pine Needle Powder on Growth Performance and Meat Quality of Chicken Thigh Meat vol.40, pp.3, 2013, https://doi.org/10.5536/KJPS.2013.40.3.187
  4. Identification of a Neolignan Glycoside from the Pine Tree, Pinus densiflora Showed Antithrombotic Activity vol.24, pp.8, 2014, https://doi.org/10.5352/JLS.2014.24.8.873
  5. Antioixdative Activity and Quality Characteristics of Rice Cupcakes Prepared with Pine Needle Powder and Extract vol.28, pp.5, 2012, https://doi.org/10.9724/kfcs.2012.28.5.613
  6. Effects of Allium victorials Extract on Lowing Lipid, Anti-oxidation and Concentration of Inflammatory Mediators in Rats Fed High Oxidized Fat vol.26, pp.2, 2013, https://doi.org/10.7732/kjpr.2013.26.2.227
  7. Effects of feed supplemented with fermented pine needles (Pinus ponderosa) on growth performance and antioxidant status in broilers vol.94, pp.6, 2015, https://doi.org/10.3382/ps/pev013
  8. A new abietic acid-type diterpene glucoside from the needles of Pinus densiflora vol.32, pp.12, 2009, https://doi.org/10.1007/s12272-009-2206-x
  9. Effects of Dietary Supplementation of Pine Needle Powder on Meat Quality and Physico-chemical Properties of Chicken Meat vol.38, pp.4, 2011, https://doi.org/10.5536/KJPS.2011.38.4.247
  10. Lipolytic Effect of Sparassis crispa Extracts in Differentiated 3T3-L1 Cells and High Fat Diet-induced Obese Mice vol.41, pp.12, 2012, https://doi.org/10.3746/jkfn.2012.41.12.1708
  11. Analysis of the Volatile Components of ThreePinusSpecies by Headspace-GC/MS vol.17, pp.6, 2014, https://doi.org/10.1080/0972060X.2014.935015
  12. Effects of Scutellariae Radix Extract on Lipid Metabolism, Oxidation and Production of Pro-Inflammatory Cytokines in Rats Fed Highly Oxidized Fat vol.16, pp.2, 2016, https://doi.org/10.15429/jkomor.2016.16.2.85
  13. Chemical Composition and Antioxidant Activity of Essential Oil of Six Pinus Taxa Native to China vol.20, pp.5, 2015, https://doi.org/10.3390/molecules20059380
  14. Effects of Ixeris dentata ext. on Lowering Lipid and Anti-oxidation vol.24, pp.1, 2011, https://doi.org/10.7732/kjpr.2011.24.1.055
  15. Comparison of chemical composition and antimicrobial activity of essential oils from three Pinus species vol.44, 2013, https://doi.org/10.1016/j.indcrop.2012.10.026
  16. Cytotoxic effects of Pinus eldarica essential oil and extracts on HeLa and MCF-7 cell lines vol.11, pp.6, 2016, https://doi.org/10.4103/1735-5362.194887
  17. The Antiobesity Effect ofPolygonum aviculareL. Ethanol Extract in High-Fat Diet-Induced Obese Mice vol.2013, 2013, https://doi.org/10.1155/2013/626397
  18. Profiling of Volatile Components Using Gas Chromatography-Mass Spectrometry in Commercial Pine Needle (Pinus densiflora S. and Z.) Powder vol.16, pp.1, 2011, https://doi.org/10.3746/jfn.2011.16.1.045
  19. Utilization of Processed Pine Needle Powder in Broiler Chicken Diets vol.45, pp.3, 2018, https://doi.org/10.5536/KJPS.2018.45.3.167
  20. 솔잎, 깻잎 및 녹차분말이 함유된 유화형 소시지 품질특성 vol.47, pp.4, 2003, https://doi.org/10.5187/jast.2005.47.4.667
  21. 2형 당뇨 마우스에서 솔잎 추출용액의 경구투여가 혈당조절에 미치는 영향 vol.35, pp.3, 2003, https://doi.org/10.3746/jkfn.2006.35.3.321
  22. 솔잎 열수추출물이 카드뮴으로 유도한 흰쥐의 산화적 손상에 미치는 영향 vol.36, pp.4, 2003, https://doi.org/10.3746/jkfn.2007.36.4.411
  23. 솔잎추출물의 피부건강 개선효과 vol.44, pp.2, 2016, https://doi.org/10.4014/mbl.1603.03006
  24. The Effects of Hydroalcoholic Extract of Pinus eldarica on Hippocampal Tissue Oxidative Damage in Pentylenetetrazole-Induced Seizures in Rat vol.13, pp.1, 2003, https://doi.org/10.2174/1573401312666161017142930
  25. 이상지질혈증 동물 모델을 이용한 솔잎 착즙액 첨가 설기떡의 지질개선 효과 vol.52, pp.1, 2019, https://doi.org/10.4163/jnh.2019.52.1.6