Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지

Insecticide Susceptibility in the Different Larva of Tobacco Cutworm, Spodoptera litural Fabricius (Lepidoptera: Noctuidae) Collected in the Soybean Fields of Milyang, Korea

밀양산 콩포장 담배거세미나방 유충의 약제에 대한 감수성

  • 배순도 (영남농업시험장 식물환경과) ;
  • 최병렬 (농업과학기술원 작물보호부 농업해충과) ;
  • 송유한 (경상대학교 농업생명과학연구원) ;
  • 김현주 (영남농업시험장 식물환경과)
  • Published : 2003.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

The susceptibility of the different larval stages of Spodoptera litura to nine insecticides was evaluated using the perilla leaf-dipping method. Median lethal concentration ($LC_{50}$) was increased with larval development in the range of 0.5 ppm to 5.6 ppm, 9.9 ppm to 27.9 ppm, 9.6 ppm to 125.1 ppm and 24.3 ppm to 546.6 ppm in the 1st, 2nd, 3rd and 4th instar, respectively. The tolerance ratio (TR), which is the TR of 90 percent lethal concentration (LC/ sub 90/) to the recommended concentration, was 0.04 to 0.8 in the 1 st, 0.2 to 7.5 in the 2nd, 0.7 to 115.3 in the 3rd and 1.2 to 485.4 in the 4th instars. Lower D$LC_{50}$ and DTR, which is the difference between the $LC_{50}$ and the TR of 4th and other instars, respectively, were observed in chlorfenapyr, chlorpyrifos and EPN while higher ones were lufenuron, chlorfluazuron and teflubenzuron. These results mean that insecticides with lower D$LC_{50}$ and DTR are effective in controlling larva of S. litura collected in Milyang, Korea.

밀양에서 채집한 담배거세미나방 유충의 약제감수성을 조사하였다. 약제의 반수치 사농도는 1령층에서 0.5-5.6ppm,2령층에서 9.9-27.9ppm, 3령층에서 9.6-125.1ppm 및 4령층에서 24.3-546.6ppm으로 약종에 따른 차이와 함께 유충의 영기가 증가할수록 높았다. 또한 약제별 추천농도에 대한 유충의 90%치사농도의 비로 나타낸 약제내성비(내성비=$LC_{90}$ 약제별 추천농도)도 1령충에서 0.04-0.8, 2령충에서 0.2-7.5, 3령충에서 0.7-115.3및 4령충 에서 1.2-483.4으로 약종에 따른 차이와 함께 유충의 영기가 증가할수록 높았다. 한편,동일 약제에서 유충의 영기간 반수치사농도, 및 내성비의 차이는 chlorfenapyr, chlorpyrifos 및 EPN에서 매우 낮았으며, lufenuron, chlorfluazuron및 teflubenzuron에서 매우 높았다. 이러한 결과를 종합하면 담배거세미나방 유충의 방제는 영기가 어릴수록 또한 약제감수성이 낮은 약제, 즉 반수치사농도 및 내성비의 차이가 적은 약제인 chlorfenapyr, chlorpyrifos 및 EPN을 사용할수록 효과적임을 의미한다.

Keywords

References

  1. Aguayo, M.I. and F.R. Villaneueva, F.R. 1985. Susceptibility of Meteorus hyphantriae Riley to emthyl parathion. Southwest. Entomol. 10: 107-109
  2. Anonymous. 2001. 5-year programs for environment-friendly agriculture. Ministry of Agriculture and Forestry. 159pp
  3. Anonymous. 2002. Pesticide guide book. Korea crop protection association 911pp
  4. Bae, S.D. and H.J. Cho. 1998. Study on the physio-biology and control of Spodoptera litura Fabricius. Pl. Environ. Res. Rept. Nat. Yeongnam Agr. Expt. Stan. RDA. pp. 834-841
  5. Bae, S.D., K.B. Park and Y.J. Oh. 1997. Effects of temperature and food source on the egg and larval devlopment of tobacco cutworm, Spodoptera litura Fabricius. Kor. J. Appl. Entomol. 36: 48-54
  6. Bae, S.D. and K.B. Park. 1999. Effects of temperature and food source on pupal development, adult longevity and ovipositionof the tobacco cutworm, Spodoptera litura Fabricius. Kor. J. Appl. Entomol. 38: 23-28
  7. Bae, S.D. 1999. Leaf characteristics of leguminous plants and the biology of tobacco cutworm, Spodoptera litura Fabricius, I. The larval development and leaf feeding amount. Kor. J. Appl. Entomol. 38: 217-224
  8. Bratisten, S.B. and R.L. Metcalf. 1973. Age-dependent variations in the response of several species of diptera to insecticidal chemicals. Pestic. Biochem. Physiol. 3: 189-198 https://doi.org/10.1016/0048-3575(73)90103-X
  9. Cho, J.R., W.R. Song, S.Y. Hwang, H.S. Kim and J.O. Lee. 1996. Age-related susceptibility of Spodoptera litura larvae to some insecticides. Kor. J. Appl. Entomol. 35: 249-253
  10. Cho, J.R., Y.J. Kim, H.S. Kim, J.K. Yoo and J.O. Lee. 1999a. Toxicity of insecticides and metabolism of deltamethrin in Helicoverpa assulta Guenee and Spodoptera litura Fabricius. J. Asia-Pacific Entomol. 2: 77-83 https://doi.org/10.1016/S1226-8615(08)60034-5
  11. Cho, J.R., Y.J. Kim, K.J. Kim, H.S. Kim, J.K. Yoo and J.O. Lee. 1999b. Electrophoretic pattern of larval esterases in field and laboratory-selected strains of the tobacco cutworm, Spodoptera litura (Fabricius). J. Asia-Pacific Entomol. 2: 39-44 https://doi.org/10.1016/S1226-8615(08)60029-1
  12. Critchley, B.R. 1972. A laboratory study on the effects of some soil-applied organophosphorus pesticide, thionazin, on predacious carabidae (Coleoptera). Bull. Entomol. Res. 62: 229-241 https://doi.org/10.1017/S0007485300047684
  13. Kim, C.H. and H.Y. Shin. 1987. Studies on bionomics and control of tobacco cutworm, Spdoptera litura Fabricius in southern part of Korea. J. Inst. Agr. Res. Util. Gyeongsang Natl, Univ. 21: 105-122
  14. Kim, Y.G., J.I. Lee, S.Y. Kang and S.C. Han. 1998a. Age variation in insecticide susceptibility and biochemical changes of beet armyworm, Spodoptera exigua (Hubner). J. Asia-Pacific Entomol. I: 109-113 https://doi.org/10.1016/S1226-8615(08)60012-6
  15. Minamikawa, H. 1937. Survey on the tobacco cutworm, Spodop tera litura Fabricius. Taiwan Central Res. Inst. Agr. Report 70: 1-66
  16. Moussa, M., A. Zaher and F. Kotby. 1960. Aboundance of the cotton leafworm, Prodenia litura (F.) (S. litoralis), in relation to host plants, I. host plants and their effect on biology (Lepidoptera: Agrotidae-Zenobiinae). Bull. Soc. Entomol. Egypt 44: 241-251
  17. Mochida, O. and T. Okada. 1974. A bibliography of Spodoptera litura spp. (Lepidoptera: Noctuidae). Misc. Bull. Kyushu Nat. Agr. Expt. Sta. 49: 1-110
  18. Nasr, E.S., M.A. Moussa and A.S. Hassan. 1960. Soil moisture in relation to population and moth emergence of the cotton leaf worm, Prodenia litura Fabricius. Bull. Soc. Entom. Egypte. XLIV: 377-382
  19. Raymond, M. 1985. Presentation d'un programme d'analyse logprobit pour micro-ordinateur. Cah. ORSTOM. Ser. Ent. Med. et Parasitol. 22: 117-121
  20. Shin, H.Y., C.H. Kim, C.G. Park and Y.S. Lee. 1987. Biology of tobacco cutworm, Spodoptera litura (F.), (Lepidoptera: Noctuidae); I. Seasonal occurrence of tobacco cutworm in southern Korea and larval development, pupal period, adult longevity and oviposition on the different food sources. Res. Rept. RDA (D . M & U) 29: 301-307
  21. Taguchi, R. 1961. On the outbreak of tobacco cutworm, Spodoptera litura Fabricius. Plant Quarantine 15: 541-542