Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Multi-step Reactions on Microchip Platform Using Nitrocellulose Membrane Reactor

  • Park, Sung-Soo (School of Chemical Engineering, Seoul National University) ;
  • Joo, Hwang-Soo (School of Chemical Engineering, Seoul National University) ;
  • Cho, Seung-Il (School of Electrical Engineering and Computer Science, Seoul National University) ;
  • Kim, Min-Su (School of Electrical Engineering and Computer Science, Seoul National University) ;
  • Kim, Yong-Kweon (School of Electrical Engineering and Computer Science, Seoul National University) ;
  • Kim, Byung-Gee (School of Chemical Engineering, Seoul National University)
  • Published : 2003.07.01
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Abstract

A straightforward and effective method is presented for immobilizing enzymes on a microchip platform without chemically modifying a micro-channel or technically microfabricating a column reactor and fluid channel network. The proposed method consists of three steps: the reconstitution of a nitrocellulose (NC) membrane on a plane substrate without a channel network, enzyme immobilization on the NC membrane, and the assembly of another substrate with a fabricated channel network. As a result, enzymes can be stably and efficiently immobilized on a microchip. To evaluate the proposed method, two kinds of enzymatic reaction are applied: a sequential two-step reaction by one enzyme, alkaline phosphatase, and a coupled reaction by two enzymes, glucose oxidase and peroxidase, for a glucose assay.

Keywords

References

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