Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Sequence and phylogenetic analysis of the phnS gene encoding 2-hydroxychromene-2-carboxylate isomerase in Sphingomonas chungbukensis DJ77

Sphingomonas chungbukensis DJ77 균주에서 2- hydroxychromene-2-carboxylate isomerase를 암호화하는 phnS 유전자의 염기서열과 상동성 분석

  • 엄현주 (충북대학교 자연과학대학 생명과학부, 바이오연구소) ;
  • 강민희 (충북대학교 자연과학대학 생명과학부, 바이오연구소) ;
  • 김영필 (충북대학교 자연과학대학 생명과학부, 바이오연구소) ;
  • 김성재 (충북대학교 자연과학대학 생명과학부, 바이오연구소) ;
  • 김영창 (충북대학교 자연과학대학 생명과학부, 바이오연구소)
  • Published : 2003.09.01
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Abstract

Sphingomonas chungbukensis DJ77 is able to metabolize phenanthrene as the sole carbon and energy source. The plasmid pUPX5 includes phnS gene encoding 2-hydroxychromene-2-carboxylate (HCCA) isomerase, which is needed for phenanthrene and naphthanene degradation. We determined the nucleotide sequence of DNA fragment of 3271 bp which included the phnS gene. The fragment included an open reading frame of 594 bp which has ATG initiation codon and TAA termination codon and GGAA ribosomal binding site. The predicted amino acid sequence of the enzyme consists of 198 amino acids. The deduced amino acid sequence of the phnS enzyme exhibited 94％ identity with that of the corresponding enzyme in Sphingomonas aromaticivorans F199. The phnS gene is located downstream and in the same operon as phnQ and phnR, encoding a 2,3-dihydroxybiphenyl 1,2-dioxygenase and a ferredoxin component of biphenyl dioxygenase, respectively.

Sphingomonas chungbuken교 DJ77은 phenanthrene을 단일 탄소원과 에너지원으로 이용하여 살아갈 수 있다. pUPXS는 phenanthrene과 naphthalene분해를 위해 필요한 2-hydroxychromene-2-carboxylate (HCCA) isomerase를 암호화하는phnS유전자를 포함한다. 본 논문에서는 phnS유전자가 포함된 3271 bp의 염기 서열을 결정하였다. 이 유전자는 ATG를 개시코돈으로 사용하며, TAA를 종결 코돈으로 사용하고 있다. 또한 개시 코돈의 5 bp앞쪽으로 GGAA라는 ribosomal binding site를 갖는다. phnS는 총 594 bP의 open reading frame을 포함하며,158개의 아미노산으로 구성되었다. phns를 구성하는 아미노산서열은 S. aromaticivorans F199의 유사서열과 87％의 유사성을 나타냈다. phns 유전자는 biphenyl dioxygenase를 구성하는 2,3-dihydroxybiphenyl 1,2-dioxygenase를 암호화하는 phnQ와 ferredoxin를 암호화 하는 phnR과 같은 operon을 이루며, 이들 유전자의 downstream에 위치하고 있다.

Keywords

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