DOI QR코드

DOI QR Code

μFIA 바이오 센서를 이용한 포도당 농도 측정

Measurement of Glucose Concentration Using a μFIA Biosensor

  • ;
  • Joseph Irudayaraj (Department of Agricultural & Biological Engineering. The Penn State University)
  • 발행 : 2003.10.01

초록

A microdialysis coupled flow injection amperometric biosensor was calibrated to measure the concentration of glucose using 7 standard samples from 10ml to 70ml of glucose solution. The output of the sensor increased linearly with an increase in the glucose concentration with an $R^2$ correlation of 0.99. The amperometric biosensor was then applied to measure the. glucose concentration of 2 commercial samples(Orange and Pineapple juice) and the results compared with HPLC. Around 12% error was observed in glucose concentration measurements of the samples analyzed. The sensor has potential in rapid measurement once the calibration is done. Potential for on-line sensing is also discussed.

키워드

참고문헌

  1. Cass, A. E. G. 1990. Biosensors-A Practical Approach. Oxford University Press, New York.
  2. Habermuller, K., M, Mosbach and W. Schuhmann. 2000. Electron-transfer mechanism in amperometric biosensors. Fresenius J. Anal. Chem. 366:560-568. https://doi.org/10.1007/s002160051551
  3. Ruzicka, J. and E. H. Hansen. 1975. Flow injection analysis. Part 1. A new concept of fast continuous flow analysis. Anal. Chim. Acta, 78:145. https://doi.org/10.1016/S0003-2670(01)84761-9
  4. Velasco-Garcia, M. N. and T. Mottram. 2003. Biosensor technology addressing agricultural problems. Biosystems Engineering. 84(1):1-12. https://doi.org/10.1016/S1537-5110(02)00236-2
  5. Vijayakumar, A. R. 1998. Amperometric Carbon Paste Biosensors Based on Horseradish Peroxidase and Oxidases. Ph.D. Thesis. Lund University, Sweden.
  6. Wang, J. 2001. Glucose biosensors:40 years of advances and challenges. Electroanalysis 13:983-988. https://doi.org/10.1002/1521-4109(200108)13:12<983::AID-ELAN983>3.0.CO;2-#