Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant and Genotoxic Inhibition Activity of Ethanol Extract from the Inonotus obliquus

차가버섯 분획물의 항산화활성 및 유전독성 억제효과

  • 함승시 (강원대학교 식품생명공학부) ;
  • 오상화 (강원대학교 식품생명공학부) ;
  • 김영균 (국민대학교 임산공학과) ;
  • 신광순 (경기대학교 식품생명공학과) ;
  • 장현유 (한국국립농업대학 버섯학과) ;
  • 정국훈 (두산그룹 생명공학연구조합)
  • Published : 2003.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to observe the antioxidative and genotoxic effect of the fractions from Inonotus obliquus using DPPH test and micronucleus assay. Stepwise fractionation of the ethanol extract from Inonotus obliquus was done by using hexane, chloroform, ethyl acetate, butanol and water to obtain effective fraction. Each fraction was tested in 1.5 ${\times}$ 10$^{-4}$ DPPH. Among six fractions, the ethyl acetate fraction showed the highest electron donating activities (46.5 $\mu\textrm{g}$/mL). The results on genotoxic effects on insoluble fractions and most of fractions showed cytotoxic effects more than 90% activity. These results suggest that some components contained in the Inonotus obliquus showed such activities and much more studies have to perform.

차가버섯 분획물의 항산화활성과 유전독성 억제효과를 규명하기 위하여 차가버섯으로부터 조다당, 불용성 조다당 I과 II를 각각 추출하였으며 그 여액에서 저분자 물질과 에틸 아세테이트, 물 분획물을 얻었다. 얻어진 시료들은 DPPH free radical 소거능으로 항산화 활성을 측정하였으며, 마우스를 이용한 소핵실험으로 유전독성 억제효과를 검토하였다. 실험결과, 에틸 아세테이트 분획물의 RC$_{50}$ 이 46.5 $\mu\textrm{g}$으로 월등히 높았으며, 물 분획물과 수용성 다당도 각각 78.3$\mu\textrm{g}$과 77.4$\mu\textrm{g}$으로 다른 분획물에 비해 높은 항산화 효과를 나타내었다. 그리고 유전독성 억제효과에서는 양성대조군에 비하여 최고 농도인 80 mg/mg에서 저분자 물질과 에틸 아세테이트 분획물, 물 분획물, 수용성 다당 그리고 불용성 다당 I 및 II에서 각각 63.9%, 72.2%, 63.9%, 88.9%, 80.6% 그리고 81.5%의 소핵생성 억제효과를 나타내었으며 다당 분획물들이 80% 이상의 높은 억제율을 나타내었다.

Keywords

References

  1. Bulatov PK, Berezina MP, Jakimov PA. 1959. Tsaga ii ee letsebnoje primenie pri rake IV. Stadii, Leningarad. p 326.
  2. Shivrina AN. 1967. Chemical characteristics of compounds extracted from Inonotus obliquus. Chem Abstr 66: 17271-17279.
  3. Kier L. 1961. Triterpenes of Poria obliqua. J Pharm Sci 50: 471-474. https://doi.org/10.1002/jps.2600500605
  4. Kahlos K, Hiltunen R. 1983. Identification of some lanostane type triterpenes from Inonotus obliquus. Acta Pharm Fenn 92: 220-224.
  5. Ludwiczak RS, Wrzeciono U. 1975. Forschungen uber die chemischen Bestandteil de Inonotus obliquus. IV. Ergosterol Rocz Chem 34: 1701-1705.
  6. Loviagina EV, Shivrina AN. 1962. On steroid compound of the Chaga fungus. Biobimija 27: 794-800.
  7. Kahlos K, Kangas L, Hitunen R. 1987. Antitumor activityof some compounds and fractions from an n-hexane extract of Inonotus obliquus. Acta Pharm Fenn 96: 33-40.
  8. Kahlos K, Lesnau A, Lange W. 1996. Preliminary tests of antiviralactivity of two Inonotus obliquus strains. Fitoterapia, VolLXVII. p 4.
  9. Saitoh A, Sato C, Niiyama K. 1996. チャガカバノアナタケの 變異原性 抑 制効果について. 道衛硏所報 第6集.
  10. Ichimura T, Watanave O, Maruyama S. 1998. Inhibition of HIV-1 protease by water-soluble lignin-like substance from an edible mushrum, Fuscoporia obliqua. Biosci Biotechnol Biochem 62: 575-577. https://doi.org/10.1271/bbb.62.575
  11. Mizuno T, Zhuang C, Abe K, Okamoto H, Kiho T, Ukai S, Leclerc S, Meijer L. 1999. Antitumor and hypohlycemic activities of polysaccharides from the Sclerotia and Mycelia of Inonotus obliquus (Pers.: Fr.) Pil. (Aphyllophoromycetideae). Int J Med Mushrooms 1: 301-316. https://doi.org/10.1615/IntJMedMushr.v1.i4.20
  12. Choi JS, Park JH, Kim HG. 1993. Screening for antioxidant activity of plants and marine algae and its active principles from Prunus daviana. Kor J Pharmacology 24: 299-303.
  13. Schmid W. 1975. The micronucleus test. Mutation Res 31: 9-15. https://doi.org/10.1016/0165-1161(75)90058-8
  14. Kim MN, Cui CB, Lee DS, Ham SS. 2001. Cytotoxicity and antigenotoxic effects of Cordyceps militaris. J Korean Soc Food Sci Nutr 30: 921-927.
  15. Chen YJ, Shiao MS, Lee SS, Wang SY. 1997. Effect of Cordyce sinensis on the proliferation and differentation of human leukemia U937 cells. Life Science 60: 2349-2359. https://doi.org/10.1016/S0024-3205(97)00291-9
  16. Kim SW. 1988. Studes on anti-microbial and anti-cancer function of polysaccharide extracted Ganoderma lucidum. J Korean Soc Food Sci Nutr 27: 1183-1188.

Cited by

  1. Development of a Beverage Using the Extracts from Bombyx mori L., Morus alba L., Dioscoreae rhizome and Inonotus obliquus vol.18, pp.6, 2011, https://doi.org/10.11002/kjfp.2011.18.6.844
  2. Masou salmon (Oncorhynchus masou) ethanol extract decreases 3-hydroxy-3-methylglutaryl coenzyme A reductase expression in diet-induced obese mice vol.29, pp.2, 2009, https://doi.org/10.1016/j.nutres.2008.11.006
  3. Antimutagenic effects of subfractions of Chaga mushroom (Inonotus obliquus) extract vol.672, pp.1, 2009, https://doi.org/10.1016/j.mrgentox.2008.10.002
  4. Antioxidative and antimutagenic activities of 70% ethanol extract from masou salmon (Oncorhynchus masou) vol.22, pp.6, 2008, https://doi.org/10.1016/j.tiv.2008.05.002
  5. Characterization and β-secretase Inhibitory Activity of Water-soluble Polysaccharides Isolated from Phellinus linteus Fruiting Body vol.40, pp.4, 2012, https://doi.org/10.4489/KJM.2012.40.4.229
  6. Chaga mushroom extract inhibits oxidative DNA damage in human lymphocytes as assessed by comet assay vol.21, pp.1-4, 2004, https://doi.org/10.1002/biof.552210120
  7. Anticancer activity of subfractions containing pure compounds of Chaga mushroom (Inonotus obliquus) extract in human cancer cells and in Balbc/c mice bearing Sarcoma-180 cells vol.4, pp.3, 2010, https://doi.org/10.4162/nrp.2010.4.3.177
  8. Antioxidative Activity of Mushroom Water Extracts Fermented by Lactic Acid Bacteria vol.43, pp.1, 2014, https://doi.org/10.3746/jkfn.2014.43.1.080
  9. Individual differences on immunostimulatory activity of raw and black garlic extract in human primary immune cells vol.34, pp.4, 2012, https://doi.org/10.3109/08923973.2011.649288
  10. Hypoglycemic and Antioxidative Effects of Fermented Chaga Mushroom (Inonotus obliquus) on Streptozotocin-induced Diabetic Rats vol.15, pp.5, 2003, https://doi.org/10.5352/jls.2005.15.5.809
  11. 차가버섯 물추출물의 대장암세포 증식억제 및 Apoptosis 유도기전 연구 vol.35, pp.5, 2003, https://doi.org/10.3746/jkfn.2006.35.5.516
  12. 버섯균사체 배양기질로서의 손바닥선인장의 활용과 그 배양추출물의 기능적 특성 vol.16, pp.3, 2003, https://doi.org/10.5352/jls.2006.16.3.477
  13. 기와층버섯 추출물의 항비만활성, 항암활성 및 단회경구독성시험 vol.23, pp.3, 2007, https://doi.org/10.5487/tr.2007.23.3.253
  14. 면섬유 및 한지섬유의 자연염색 및 기능성광물처리에 의한 쾌적성의 변화 vol.12, pp.2, 2010, https://doi.org/10.5805/ksci.2010.12.2.219