Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Modification of Gamma-radiation Response in Mice by Green Tea and Diethyldithiocarbamate

마우스에서 방사선 영향에 대한 녹차와 Diethyldithiocarbamate의 조절효과

  • Kim, Se-Ra (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Hae-June (College of Veterinary Medicine, Chonnam National University) ;
  • Oh, Heon (Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Ryu, Si-Yun (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Yun-Sil (Dept. of Radiation Response, Korea Cancer Center Hospital) ;
  • Kim, Tae-Hwan (Dept. of Radiation Response, Korea Cancer Center Hospital) ;
  • Jo, Sung-Kee (Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute)
  • 김세라 (전남대학교 수의과대학) ;
  • 김성호 (전남대학교 수의과대학) ;
  • 이해준 (전남대학교 수의과대학) ;
  • 오헌 (한국원자력연구소 방사선식품.생명공학팀) ;
  • 류시윤 (충남대학교 수의과대학) ;
  • 이윤실 (원자력의학원) ;
  • 김태환 (원자력의학원) ;
  • 조성기 (한국원자력연구소 방사선식품.생명공학팀)
  • Published : 2003.10.01
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Abstract

We performed this study to determine the effect of green tea on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells of mice irradiated with high and low dose of gammairradiation. The radioprotective effect of green tea was compared with the effect of diethyldithiocarbamate (DDC). Jejunal crypts were protected by pretreatment of green tea (p<0.01). Green tea administration before irradiation resulted in an increase of the formation of endogenous spleen colony (p<0.05). The frequency of radiation-induced apoptosis in intestinal crypt cells was also reduced by pretreatment of green tea (p<0.05). The radioprotective effect on jejunal crypts and apoptosis in the DDC treated group appeared similar to those in the green tea treated groups. Treatment with DDC showed no significant modifying effects on the formation of endogenous spleen colony. These results indicated that green tea might be a useful radioprotector, especially since it is a relatively nontoxic natural product. Further studies are needed to characterize better the promotion nature of green tea and its components.

본 연구에서는 방사선 손상 경감효과를 나타내는 기호식품을 발굴하고자 국내에서 주로 음용되고 있는 녹차의 방사선 손상 경감효과를 확인하기 위하여 고선량 및 저선량 방사선을 마우스에 조사하고 소장움 생존, 내재성 비장 조혈세포집락 형성 및 apoptosis 유발 등을 관찰하였으며, 그 효과를 방사선 방호효과가 알려져 있는 diethyldithiocarbamate(DDC)와 비교$.$관찰하였다. 생존 소장움의 수는 방사선 조사 전 녹차 투여군(경구투여군 : p<0.01, 복강내 투여군 : p<0.01) 및 DDC 투여군(p<0.005)에서 유의성있게 증가되었다. 내재성 조혈세포집락 형성은 방사선 조사 대 조군에 비하여 조사 전 복강내 투여한 군(p<0.05)에서만 유의성 있는 증가를 나타냈으며, DDC 투여군에서는 효과를 관찰할 수 없었다. 방사선에 의한 apoptotic cell의 형성은 방사선 조사 전 녹차 투여군(경구 투여군 : p<0.005, 복강내 투여군 : p<0.05)및 DDC투여군 (p<0.005)에서 통계적으로 유의성 있게 감소되었다. 이상의 결과에서 녹차의 방사선 손상 경감효과를 조혈세포의 생존과 회복, 소장움 세포 생존 및 방사선에 의한 apoptosis 유발억제를 통하여 확인하였으며, 이는 독성이 적은 천연물이라는 관점에서 방사선 손상 경감 음료로서 적용이 가능할 것으로 사료되며 추후 유효 분획 및 성분에 관한 연구가 요구된다.

Keywords

References

  1. IAEA safety series No. 47. 1978. Manual on Early Medical Treatment of Possible Radiation Injury. IAEA, Vienna. p 74.
  2. NCP report No. 65 1980. Management of Persons Accidentally Contaminated with Radionuclides. p 77.
  3. Patt H, Tyree M, Straube RL. 1949. Cystein protects against x-irradiation. Science 110: 213-214. https://doi.org/10.1126/science.110.2852.213
  4. Milas L, Hunter N, Reid BO, Thames Jr HD. 1982. Protective effects of S-2-(3-aminopropylamino) ethylphosphorothioic acid against radiation damage of normal tissues and a fibrosarcoma in mice. Cancer Res 42: 1888-1987.
  5. Milas L, Murray D, Brock WA, Meyn RE. 1988. Radioprotectors in tumorradiotherapy: Factors and settings determining therapeutic ratio. Pharmacol Ther 39: 179-189. https://doi.org/10.1016/0163-7258(88)90059-9
  6. Neta R, Douches S, Oppenheim JJ. 1986. Interleukin 1 is a radioprotector. J Immunol 136: 2483-2485.
  7. Neta R. 1988. Role of cytokines in radioprotection. Pharmacol Ther 39: 261-266. https://doi.org/10.1016/0163-7258(88)90070-8
  8. MacVittie TJ, Monroy RL, Patchen ML, Souza LM. 1990. Therapeutic use of recombinant human G-CSF (rhG-CSF) in a canine model of sublethal and lethal whole body irradiation. Int J Radiat Biol 57: 723-736. https://doi.org/10.1080/09553009014550891
  9. Sweeney TR. 1979. A survey of compounds from the antiradiation drug development program of the U.S. army medical research & development command. Walter Reed Army Institute of Research. Washington, DC.
  10. Kligerman MM, Shaw MT, Slavid M, Yudas JM. 1980. Phase I clinical studies with WR2721. Cancer Clin Trials 3: 217-221.
  11. Sohn KH, Lee MJ, Min SH, Lee HJ. 2000. A study on the factors affecting the consumption of coffee and tea among female in Seoul. Korean J Dietary Culture 15: 398-412.
  12. Mathieu J, Ferlat S, Ballester B, Platel S, Herodin F, Chancerelle Y, Mestries JC, Kergonou JF. 1996. Radiation-induced apoptosis in thymocytes: inhibition by diethyldithiocarbamate and zinc. Radiat Res 146: 652-659. https://doi.org/10.2307/3579381
  13. Rencova J, Svoboda V, Holusa R, Volf V, Jones MM, Singh PK. 1997. Reduction of subacute lethal radiotoxicity of polonium-210 in rats by chelating agents. Int J Radiat Biol 72: 341-348. https://doi.org/10.1080/095530097143338
  14. Hall EJ. 1988. Radiobiology for the radiologist. 4th edition. J.B. Lippincott Company, New York. p 48-49.
  15. Washburn LC, Carlton JE, Hayes RL. 1974. Distribution of WR-2721 in normal and malignant tissue of mice and rats bearing solid tumors: dependence on tumor type, drug dose and species. Radiat Res 59: 483-575.
  16. Cairnie AB. 1983. Adverse effect of radioprotector WR2721. Radiat Res 94: 221-226. https://doi.org/10.2307/3575878
  17. Yuan Y, Hou S, Lian T, Han Y. 1992. Studies of Rehmannia glutinosa Libosch. f. hueichingensis as a blood tonic. Chung Kuo Chung Yao Tsa Chih 17: 366-368.
  18. Miyanomae T, Frindel E. 1988. Radioprotection of hemopoiesis conferred by Acanthopanax senticosus Harms (Shigoka) administered before or after irradiation. Exp Hematol 16: 801-806.
  19. Wang Y, Zhu B. 1996. The effect of angelica polysaccharide on proliferation and differentiation of hematopoietic progenitor cell. Chung Hua I Hsueh Tsa Chih 76: 363-366.
  20. Zneg XL, Li XA, Zhang BY. 1992. Immunological and hematopoietic effect of Codonopsis pilosula on cancer patients during radiotherapy. Chung Kuo Chung Hsi I Chieh Ho Tsa Chih 12: 607-608.
  21. Wang HB, Zheng QY, Ju DW, Fang J. 1993. Effects of phytolaccaacinosa polysaccharides II on lymphocyte proliferation and colony stimulating factor production from mice splenocytes in vitro. Yao Hsueh Hsueh Pao 28: 490-493.
  22. Hsu HY, Hau DM, Lin CC. 1993. Effects of kuei-pi-tang on cellular immunocompetence of gamma-irradiated mice. Am J Chin Med 21: 151-158. https://doi.org/10.1142/S0192415X93000182
  23. Lu G, Yang M, Shen Y, Meng J. 1991. The absorption of Fe, Zn, Cu in siwu, sijunzi, and Liuwei dihuang decoction by small intestine in rats. Chung Kuo Chung Yao Tsa Chih 16: 297-298.
  24. Mitscher LA, Jung M, Shankel D, Dou JH, Steele L, Pillai SP. 1997. Chemoprotection: a review of the potential therapeutic antioxidant properties of green tea (Camellia sinensis) and certain of its constituents. Med Res Rev 17: 327-365. https://doi.org/10.1002/(SICI)1098-1128(199707)17:4<327::AID-MED2>3.0.CO;2-Y
  25. Sato T, Miyata G. 2000. The nutraceutical benefit, part I: green tea. Nutrition 16: 315-317. https://doi.org/10.1016/S0899-9007(99)00301-9
  26. Dufresne CJ, Farnworth ER. 2001. A review of latest research findings on the health promotion properties of tea. J Nutr Biochem 12: 404-421. https://doi.org/10.1016/S0955-2863(01)00155-3
  27. Cotelle N, Bernier JL, Henichart JP, Catteau JP, Gaydou E, Wallet JC. 1992. Scavenger and antioxidant properties of ten synthetic flavones. Free Radic Biol Med 13: 211-219. https://doi.org/10.1016/0891-5849(92)90017-B
  28. Guo Q, Zhao B, Li M, Shen S, Xin W. 1996. Studies on protective mechanisms of four components of green tea polyphenols against lipid peroxidation in synaptosomes. Biochim Biophys Acta 1304: 210-222. https://doi.org/10.1016/S0005-2760(96)00122-1
  29. Dreosti IE. 1996. Bioactive ingredients: antioxidants and polyphenols in tea. Nutr Rev 54: 51-58.
  30. Zloch Z. 1996. The role of dietary plant polyphenols in health maintenance. Cas Lek Cesk 135: 84-88.
  31. Weisburger JH. 1999. Tea and health: the underlying mechanisms. Proc Soc Exp Biol Med 220: 271-275 https://doi.org/10.1046/j.1525-1373.1999.d01-46.x