Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Bioactive Functions of Detoxified Puffer Liver Oil

제독처리한 복어 간유의 생리활성

  • 최종원 (경성대학교 약학대학 약학과) ;
  • 김나영 (동아대학교 식품영양학과) ;
  • 김동수 (경성대학교 식품공학과)
  • Published : 2003.10.01
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Abstract

In this study, we investigated the effects of detoxified puffer liver (PL) oil on fatigue, hepatotoxicity and hyperlipidemia. There are no toxicities in both raw and purified PL oil. The test of swimming time was extended in detoxified PL oil pretreated group compared to the non-treated group. When rats treated with PL oil, the hepatic injuries induced by carbon tetrachloride or DL-galactosamine were reduced. The increased serum triglyceride and total cholesterol by poloxamer-407 were lowered by treating with PL oil remarkably. Also the bleeding time of hyperlipidemic animals was extended and plasma clotting time was delayed by PL oil.

인류는 질병에 대한 예방 및 치료에 주로 합성 의약품을 사용하여 왔으나 근래에 이르러 여러 가지 합성 의약품의 부작용 또는 독성이 밝혀짐에 따라 세계적으로 천연자원으로부터의 의약품 개발이 활발히 추진되고 있다. 한편, 우리나라를 위시한 동양에서는 오랜 시일을 통하여 많은 식물을 치료제로 사용하여 왔으나 약화학적 및 약리학적 검토 자료는 지금에 와서 많이 도출되고 있으며 다양한 연구가 이루어지고 있는 실정이다. 본 연구에서는 해양생물로부터 생리활성을 검색할 목적으로 검은 밀복으로부터 제독처리하여 추출, 정제한 검은 밀복 간유를 대상으로 항피로효과, 간기능에 미치는 영향 및 poloxamer-407로 유도되는 고지혈증에 대한 효과를 검색한 결과, 다음과 같은 결론을 얻었다. 첫째, 식품위생학적 안전성 여부를 조사하기 위하여 검은밀복 간유의 정제품을 mouse assay로 그 잔류 독성을 조사한 결과 모두 무독하였다. 둘째, 생쥐를 유영하게 하여 사망하는 시간을 측정하여 본 결과 정상 생쥐에서는 사망시간보다 복어간유를 용량별로 2주일간 경구투여하였을때 사망 시간은 용량 의존적으로 증가되었다. 셋째, 간장해의 유발 약물로 사염화탄소 및 D-galactosamine(GaIN)을 사용하여 복어로부터 추출한 복어간유를 투여한 결과 사염화탄소 및 GaIN을 투여하였을 때 혈중 AST, ALT 및 SDH의 활성이 정상군에 비하여 현저히 증가되던 것이 복어간유의 전처리로 현저히 억제되었다. 넷째, 혈청 중 중성지방 및 total cholesterol의 함량은 정상군에 비하여 poloxamer-407의 투여로 증가되던 것이 복어간유를 용량별로 투여함으로써 현저히 감소되었다. 한편 poloxamer-407로 유도된 고지혈증의 실험동물의 bleeding time이 복어간유의 투여로 연장되었으며 plasma clotting time도 증가되었다.

Keywords

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