한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제32권7호
  • /
  • Pages.1164-1167
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  • 2003
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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국내 유통 한약재 중 Aristolochic Acid분석

Detection of Aristolochic Acid from Medicinal Herbs Marketed

  • 발행 : 2003.10.01
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초록

국내 유통 중인 한약재에 발암물질로 알려진 aristolochic acid 함유 여부를 검사하고자 방기, 목통, 목향, 마두령, 세신, 철목향 등을 대상으로 aristolochic acid 검출 유무 및 정량분석을 수행하였다. 시료에 대한 TLC와 $C_{18}$ column을 이용한 HPLC의 aristolochic acid 최적 분석 조건을 수립하였고 aristolochic acid 정량분석 결과 방기 type II에는 3.9 mg/g, 마두령에는 2.3 mg/g이 함유되어 있는 것으로 나타났다. 그외 목향, 목통, 세신, 청목향 등 시료에서는 aristolochic acid가 검출되지 않았다.

Aristolochic acid has been known to be a carcinogenic compound and to cause Chinese herbs nephrophathy(CHN). To determine the content of aristolochic acid in various medicinal herbs marketed domestically, samples of Aristolochia fangchi, Aristolochia fructus, Aristolochia radix, Asiasari radix, Saussureae radix, and Akebia caulis were analyzed using TLC and HPLC. The optimal conditions for TLC and HPLC using $C_{18}$ column were established for the quantitative analysis of aristolochic acid. This study showed that Aristolochia fangchi and Aristolochia fructus examined in this study contain aristolochic acid of 3.9 and 2.3 mg/g sample, respectively. In contrast, aristolochic acid was not detected in the other samples.

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참고문헌

  1. Vanhaelen M, Vanhaelen-Fastre R, But P, Vanherweghem J. 1994. Identification of aristolochic acid in Chinese herbs. Lancet 343: 174.
  2. Arlt VM, Stiborova M, Schmeiser HH. 2002. Aristolochic acid as a probable human cancer hazard in herbal remedies: a review. Mutagenesis 17: 265-277. https://doi.org/10.1093/mutage/17.4.265
  3. Gotzl E, Schimmer O. 1993. Mutagenicity of aristolochic acids (I, II) and aristolochic acid I in new YG strains in Salmonella typhimurium highly sensitive to certain mutagenic nitroamines. Mutagenesis 8: 17-22. https://doi.org/10.1093/mutage/8.1.17
  4. Lord GM, Tagore R, Cook T, Gower P, Pusey CD. 1999. Nephropathy caused by Chiness herbs in the UK. Lancet 354: 481-482.
  5. Pezzuto JM, Swanson SM, Mar W, Che CT, Cordell GA, Fong HH. 1998. Evaluation of the mutagenic and cytostatic potential of aristolochic acid (3,4-methylenedioxy-8-methoxy-10-nitrophenanthrene-1-carboxylicacid) and several of its derivatives. Mutation Res 206: 447-454.
  6. Nortier JL, Martinez MM, Schmeiser HH, Arlt VM, Bieler CA, Petein M, Depierriux MF, Pauw LD, Abramowicz D, Vereerstraeten P, Vanherweghem J. 2000. Urothelial carcinoma associated with the use of a Chinese herb (Aristolochia fangchi). New Eng J Med 342: 1686-1692. https://doi.org/10.1056/NEJM200006083422301
  7. Choi H, Lee S, Suh Y. 1997. PCR-mediated RFLP to identify 'Bangpoong' a crude drug. Korean J Pharm 28: 1-8.
  8. Hashimoto K, Higuchi M, Makino B, Sakakibara I, Kubo M, Komatsu Y, Maruno M, Okada M. 1999. Quantitative analyisis of aristolochic acids, toxic compounds, contained in some medicinal plants. J Ethnopharm 64: 185-189. https://doi.org/10.1016/S0378-8741(98)00123-8
  9. Hoe OS, Lee JH, Kim SE, Joo IS, Shin DW, Lee JH, Kim JY, Kang SK, Lee HK, Paek SW, Moon BW, Kim JS. 2000. Chemical analysis of circulated Sinomeni stem. Ann Report KFDA 4: 111-118.
  10. Ze KR, Sung RS, Lee JP, Park SY, Jung YJ, Cho CH, Cho SY, Ha KW, Lee HS, Lee HK. 2001. Quantitative analysis of aristolochic acids in circulating herbal medicines in Korea. Ann Report KFDA 5: 167-174.

피인용 문헌

  1. Introduction of evidence-based practical medicine through safety classification for herbal medicine(1) vol.35, pp.1, 2014, https://doi.org/10.13048/jkm.14011