한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제32권7호
  • /
  • Pages.1172-1175
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  • 2003
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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감마선 조사된 난백 함유 White Layer Cake의 돌연변이원성 평가

Safety Evaluation on Mutagenicity of White Layer Cake Containing Gamma-Irradiated Egg White

  • 김미정 (안양대학교 식품영양학과) ;
  • 이주운 (한국원자력연구소 방사선식품생명공학기술개발) ;
  • 서지현 (한국원자력연구소 방사선식품생명공학기술개발) ;
  • 송현파 (한국원자력연구소 방사선식품생명공학기술개발) ;
  • 육홍선 (충남대학교 식품영양학과) ;
  • 최정미 (건양대학교 미용디자인학과) ;
  • 변명우 (한국원자력연구소 방사선식품생명공학기술개발)
  • 발행 : 2003.10.01
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초록

감마선 조사 기술을 이용하여 계란의 알러지성을 감소시키기 위한 실증 연구의 일환으로 제조한 감마선 조사된 난백 함유 white layer cake의 독성학적 안전성을 검증하고자 본 시험을 실시하였다. 시험 시료의 물 가용분 및 methanol 가용분을 대상으로 in vitro 단기 시험인 S. typhimurium TA98과 TA100을 이용한 유전자 복귀돌연변이 시험(Ames test) 결과, 감마선 조사 난백 함유 white layer cake이 직접변이원이나 간접변이원으로 작용하지 않음을 알 수 있었다.

Mutagenicity of white layer cake including 20 kGy-gamma irradiated egg white manufactured as a research on the practical approaches of gamma irradiation for the reduction of egg allergy was evaluated by Salmonella typhimurium reversion assay (Ames test). The water-soluble and organic solvents mixture of methanol: chloroform (2 : 1)-soluble fractions of the white layer cake including 20 kGy-gamma irradiated egg white were examined in S. typhimurium TA98 and TA100. Both with and without metabolic activation, the number of revertant colonies were not increased in each extract compared with negative controls. No significant difference in the formation of the colonies was observed at the non-irradiated and 20 kGy-irradiated samples. The results indicate that there is no evidence of mutagenicity in white layer cake including 20 kGy-gamma irradiated egg white.

키워드

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피인용 문헌

  1. Scientific Opinion on the Chemical Safety of Irradiation of Food vol.9, pp.4, 2011, https://doi.org/10.2903/j.efsa.2011.1930