Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
권호 표지

Effects of Subacute Oral Administration of Bisphenol A on the IgM-PFC and Proliferation of Splenocytes in Mice

마우스에서 Bisphenol A의 아급성노출이 IgM-PFC형성능과 비장세포 증식능에 미치는 영향

  • 변정아 (숙명여자대학교 약학대학) ;
  • 표명윤 (숙명여자대학교 약학대학)
  • Published : 2003.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

To determine whether or not bisphenol A affects the Immune system, female ICR mice were treated bisphenol A (BPA) orally at the doses of 100, 500 and 1,000 mg/kg for 30 consecutive days. Four days before enumerating Plaque -forming cells (PFCs) mice were immunized intraperitoneally with sheep red blood cells (SRBCs). The spleen cellularity and PFC/spleen were significantly reduced by 30-day exposure to BPA (1,000 mg/kg/day), but the PFC/10$\^$6/ spleen cells was slightly decreased.. When splenocytes isolated from the mice exposed to BPA for 30 days were cultured in the presence of LPS, Con A or PHA with IL-2, the lymphocyte proliferation ex vivo was not significantly suppressed by BPA. Our present results indicated that 30-day exposure of mice to BPA might have mild immunotoxic potential.

Keywords

References

  1. 표명윤, 변정아. Bisphenol A의 급성노출이 마우스의 면역 기능에 미치는 영향. 약학회지 2001; 45(1): 55-63
  2. Chen MY, Ike M and Fujita M. Acute toxicity, Mutagenicity, and Estrogenecity of Bisphenol-A and other bisphenols. Environ. Toxicol, 2002; 17: 80-86 https://doi.org/10.1002/tox.10035
  3. Cunningham A and Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. 1968; Immunol., 14: 599-600
  4. Dechaud H, Ravard C, Claustrat F, Aude Brae de la perriere and Pugeat M. Xenoestrogen interaction with human sex hormone-binding globulin (hSHBG). Steroids, 1999; 64: 328-334 https://doi.org/10.1016/S0039-128X(98)00114-7
  5. Deyo JA and Kerkvliet NI. Immunotoxicity of the pyrrolizidine alkaloid monocrotaline following subcronic administration to C57B1/6 mice. Fundam. Appl. Taxicol., 1990; 14: 842-849 https://doi.org/10.1016/0272-0590(90)90308-7
  6. Dolye A, Griffiths JB and Newell DG. Cell & Tissue Culture; Laboratory procedures, 1993; John Wiily & Sons Ltd., U.K
  7. EPA-Health Effects Test Guidelines-Immunotoxicity (1996)
  8. Farabollini F, Porrini S, Seta DD, Bianchi F and Dessi-Fulgheri F. Effects of perinatal exposure to bisphenol A on sociosexual behavior of female and male rats. Environmental Health Perspectives, 2002; 110(suppl 3): 409-414 https://doi.org/10.1289/ehp.02110s3409
  9. Goodson A, Summerfield W and Cooper I. Survey of bis-phenol A and bisphenol F in canned foods. Food Addit Contam, 2002; 19(8): 796-802 https://doi.org/10.1080/02652030210146837
  10. Hiliard CA, Armstrong MJ, Bradt CI, Hill RB, Greenwood SK and Galloway SM. Chromosome aberrations in vitro related to cytotoxicity of nonmutagenic chemicals (nd metabolic poisons. Environmenatal and molecular mutagenesis, 1998; 31: 316-326 https://doi.org/10.1002/(SICI)1098-2280(1998)31:4<316::AID-EM3>3.0.CO;2-G
  11. Kang J and Kondo F. Determination of bisphenol A in canned pet foods. Res Vet Sci, 2002; 73(2): 177-183 https://doi.org/10.1016/S0034-5288(02)00102-9
  12. Krishnan AV, Stathis P, Permuth SF, Tokes L and Feldman D. Bisphenol-A: An estrogenic substance is released from polycarbonate flasks during autoclaving. Endo. 1993; 132: 2279-2286 https://doi.org/10.1210/en.132.6.2279
  13. Mishell RI and Dutton RW. Immunization of normal mouse spleen cell suspensions in vitro. Science., 1966; 153: 1004-1005 https://doi.org/10.1126/science.153.3739.1004
  14. Morrissey RE, George JD, Price CJ, TYL RW, Marr MC and Kimmel CA. The developmental toxicity of bisphenol A in rats and mice. Fundam. Appl. Toxicol., 1987; 8: 571-582 https://doi.org/10.1016/0272-0590(87)90142-4
  15. Mountfort KA, Kelly J, Jickells SM and Castle L. Investigations into the potential degradation of polycarbonate baby bottles during sterilization with consequent release of bisphenol A. Food Addit Contam, 1997; 14(6-7): 737-740 https://doi.org/10.1080/02652039709374584
  16. Olea N, Pulgar R, Perez P, Olea-Serrano F, Rivas A, Novillo-Fertrell A, Pedreaza V, Soto AM and Sonnens-chein C. Estrogenecity of resinbased composites and sealants used in dentistry. Environmental Health perspectives, 1996; 104(3): 298-305 https://doi.org/10.2307/3432888
  17. Papaconstantinou AD, Umbreit TH, Fisher BR, Goering PL, Lappas NT and Brown KM. Bisphenol A-induced increase in uterine weight and alteration in uterine morphology in ovariectomized B6C3FI mice: role of the estrogen receptor. Toxicol Sci., 2000; 56: 332-339 https://doi.org/10.1093/toxsci/56.2.332
  18. Sakurai H, Maeda M, Miyahara K, Nakayama M, Murayama H, Hasegawa H, Hayakawa M, Sugiura T and Kamikura K. Extraction of bisphenol-A from a cardiopulmonary bypass circuit. Kyobu Geka, 2002; 55(9): 770-772
  19. Tallarida RJ and Murray RB. Manual of Pharmacologic calculations with computer programs, 1981; Springer-Verlag