Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Influence of Human Follicular Fluid for IVF on PN Grade and Development of Human Embryos

사람 난포액에 의한 정자 처리가 체외수정란의 전핵과 발달에 미치는 영향

  • B. G. Jeon (Kaya Mother-Child's Hospital) ;
  • Lee, S. L. (Institute of Animal Science, College of Veterinary Medicine, Gyeongsang National University) ;
  • S. A. Ock (Department of Biology, Gyeongsang National University) ;
  • Kim, K. S. (Kaya Mother-Child's Hospital) ;
  • J. S. Moon (Kaya Mother-Child's Hospital) ;
  • D. O. Kwack (Department of Biology Education, Gyeongsang National University) ;
  • Park, G. J. (Institute of Animal Science, College of Veterinary Medicine, Gyeongsang National University) ;
  • S. Y. Choe (Institute of Animal Science, College of Veterinary Medicine, Gyeongsang National University)
  • Published : 2003.08.01
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Abstract

본 연구는 사람의 시험관아기 프로그램에서 체외 수정란의 질을 향상시키는 한 방법으로 난포액으로 처리된 정자를 체외 수정에 사용하여 생산된 체외 수정란의 전핵 등급과 발달 능력을 조사하였다. 정상적인 시험관 아기 시술을 시행한 실례 환자를 대상으로 과배란을 유기하여 배란 직전의 난자를 채취하여, 난포액으로 처리된 정자와 체외 수정시킨 후 사람 체외 수정란의 전핵 등급과 체외발달율을 조사하였다. 체외수정을 위한 정자의 처리 방법으로 synthetic serum substitute (SSS)를 15% 첨가한 modified human total fluid (m-hTF) 혹은 난포액에서 정자를 2시간 동안 swimming-up 처리 후 각각 체외수정에 사용한 결과, 수정율은 75.3 및 82.1%를 나타내어 유의적인 차이는 없었으나, 1등급 전핵란은 각각 48.0 및 65.5%를 나타내어 난포액에서 유의적으로 (P<0.05) 높았고, 배양 후 3일째에 수정란의 등급을 조사한 결과, 1등급 체외수정란은 각각 44.9 및 60.5%를 나타내어 난포액에서 유의적으로 (P<0.05) 높았다. 또한 체외수정란을 배양 후 3일째에 수정란의 발달 단계를 조사 한 결과, 5-세포기 단계 이상을 발달하는 비율은 각각 51.0 및 70.5%를 나타내어 난포액에서 유의적으로 (P<0.05) 높았다.

Keywords

References

  1. Bajamonte M, Ruvolo G, Cimino C and Cittadini E. 1994. Advantages of treatment with human follicular fluid in the management of severely dyspermic patients in human in vitro fertilization programs. Acta. Eur. Fertil., 25(2):87-92
  2. Battaglia DE, Koehler JK, Klein NA and Tucker MJ. 1997. Failure of oocyte activation after intracytoplasmic sperm injection using roundheaded sperm. Fertil. Steril., 68(1):118-22 https://doi.org/10.1016/S0015-0282(97)81486-0
  3. Brucker C and Lipford GB. 1995. The human sperm acrosome reaction: physiology and regulatory mechanisms. An update. Hum. Reprod., Update, 1 (1):51-62 https://doi.org/10.1093/humupd/1.1.51
  4. Burkman LJ, Coddington CC, Franken DR, Krugen TF, Rosenwaks Z and Hogen GD. 1998. The hemizona assay (HZA): development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential. Fertil. Steril., 49 (4):688-97
  5. Chiu WW and Chamley LW. 2002. Antibodybinding proteins in human seminal plasma. Am. J. Reprod. Immunol., 48(4):269-74 https://doi.org/10.1034/j.1600-0897.2002.01122.x
  6. Choi YH, Takagi M, Kamishita H, Wijayagunawardane MP, Acosta TJ, Miyazawa K and Sato K. 1998. Developmental capacity of bovine oocytes matured in two kinds of follicular fluid and fertilized in vitro. Anim. Reprod. Sci., 27; 50(1-2):27-33 https://doi.org/10.1016/S0378-4320(97)00087-0
  7. Coddington CC, Oehninger SC, Olive DL, Franken DR, Kruger TF and Hodgen GD. 1994. Hemizona index (HZI) demonstrates excellent predictability when evaluating sperm fertilizing capacity in in vitro fertilization patients. J. Androl., 15(3):250-4
  8. Fabbri R, Porcu E, Lenzi A, Gandini L, Marsella T and Flamigni C. 1998. Follicular fluid and human granulosa cell cultures: influence on sperm kinetics parameters, hyperactivation and acrosome reaction. Fertil. Steril., 69:112-7 https://doi.org/10.1016/S0015-0282(97)00421-4
  9. Florman HM, Corron ME, Kim TD and Babcock DF. 1992. Activation of voltage-dependent calcium channels of mammalian sperm is required for zona pellucida-induced acrosomal exocytosis. Dev. BioI., 152(2):304-14 https://doi.org/10.1016/0012-1606(92)90137-6
  10. Franco JG Jr, Baruffi RL, Mauri AL, Petersen CG and Oliveira JB. 1994. Ovarian synchrony factor: a new ultrasound parameter in the prognosis of follicular rupture. Hum. Reprod., 9(7): 1250-2 https://doi.org/10.1093/oxfordjournals.humrep.a138688
  11. Giorlandino C, Calugi G, Iaconianni L, Santoro ML and Lippa A. 1998. Spermatozoa with chromosomal abnormalities may result in a higher rate of recurrent abortion. Fertil. Steril., 70(3) : 576-7 https://doi.org/10.1016/S0015-0282(98)00192-7
  12. Harlow CR, Cahill DJ, Maile LA, Talbot WM, Mears J, Wardle PG and Hull MG. 1996. Reduced preovulatory granulosa cell steroidogenesis in women with endometriosis. J. Clin. Endocrinol. Metab., 81(1):426-9 https://doi.org/10.1210/jc.81.1.426
  13. Hourani CL, Check JH, Baker AF, Hoover LM, Summers DC and Benfer KM. 1995. Cumulus removal and addition of follicular fluid possibly improves pregnancy rates with in vitro fertilization for male factor. Arch. Androl., 34 (1):47-52 https://doi.org/10.3109/01485019508987830
  14. Huyser C, Fourie FR and Moolman H. 1997. The influence of sera, follicular fluids and seminal plasma on human sperm-zona pellucida binding. Hum. Reprod., 12(4):792-9 https://doi.org/10.1093/humrep/12.4.792
  15. Jeon BG, Moon JS, Kim KC, Lee HJ, Choe SY and Rho GJ. 2001. Follicular fluid enhances sperm attraction and its motility in human. J. Assist. Reprod. Genet., 18(8):407-12 https://doi.org/10.1023/A:1016674302652
  16. Kimura Y, Yanagimachi R, Kuretake S, Bortkiewicz H, Perry AC and Yanagimachi H. 1998. Analysis of mouse oocyte activation suggests the involvement of sperm perinuclear material. BioI. Reprod., 58(6):1407-15 https://doi.org/10.1095/biolreprod58.6.1407
  17. Lambert H, Steinleitner A, Eisermann J, Serpa N and Cantor B. 1992. Enhanced gamete interaction in the sperm penetration assay after coincubation with pentoxifylline and human follicular fluid. Fertil. Steril., 58(6):1205-8 https://doi.org/10.1016/S0015-0282(16)55570-8
  18. Marin-Briggiler CI, Tezon JG, Miranda PV and Vazquez-Levin MH. 2002. Effect of incubating human sperm at room temperature on capacitation-related events. Fertil. Steril., 77(2):252-9 https://doi.org/10.1016/S0015-0282(01)02982-X
  19. Minami S, Yamano S, Ishikawa H and Aono T. 1995. Increase of intracellular free [Ca2+] in single human motile spermatozoa treated with human follicular fluid. Arch. Androl., 34(3):115-23 https://doi.org/10.3109/01485019508987839
  20. O'Toole CM, Roldan ER and Fraser LR. 1996. Protein kinase C activation during progesterone-stimulated acrosomal exocytosis in human spermatozoa. Mol. Hum. Reprod., 2(12): 921-7 https://doi.org/10.1093/molehr/2.12.921
  21. Parinaud J, Vieitez G, Labal B and Richoilley G. 1994. Effect of sperm pre-treatments on the results of sub-zonal insemination (SUZI). Hum. Reprod., 9(1):110-2 https://doi.org/10.1093/humrep/9.suppl_4.110
  22. Parrington J, Swann K, Shevchenko VI, Sesay AK and Lai FA. 1996. Calcium oscillations in mammalian eggs triggered by a soluble sperm protein. Nature, 25;379(6563):364-8 https://doi.org/10.1038/379364a0
  23. Qiao J, Yeung WS, Yao YQ and Ho PC. 1998. The effects of follicular fluid from patients with different indications for IVF treatment on the binding of human spermatozoa to the zona pellucida. Hum. Reprod., 13(1):128-31 https://doi.org/10.1093/humrep/13.1.128
  24. Quinn P, Kerin JF and Warnes GM. 1985. Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil. Steril., 44(4):493-8 https://doi.org/10.1016/S0015-0282(16)48918-1
  25. Ralt D, Goldenberg M, Fetterolf P, Thomson D, Dor J, Mashiach S, Garbers DL and Eisenbach M. 1991. Sperm attraction to a follicular factor(s) correlates with human egg fertilizability. Proc. Nat. Acad. Sci., 88:2840-4 https://doi.org/10.1073/pnas.88.7.2840
  26. Rufas O, Gilman A, Fisch B and Shalgi R. 1998. Spontaneous and follicular fluid-induced acrosome reaction in sperm samples from in vitro fertilizing and nonfertilizing normozoospermic patients.J. Assist. Reprod. Genet., 15(2):84-9 https://doi.org/10.1007/BF02766831
  27. Shibahara H, Hirano Y, Takamizawa S and Sato I. 2003. Effect of sperm-immobilizing antibodies bound to the surface of ejaculated human spermatozoa on sperm motility in immunologically infertile men. Fertil. Steril., 79(3): 641-2 https://doi.org/10.1016/S0015-0282(02)04702-7
  28. Shiomi H, Yamano S, Shono M and Aono T. 1996. Characteristics of calcium ion influx induced by human follicular fluid in individual human sperm. Arch. Androl., 37(2):79-86 https://doi.org/10.3109/01485019608988506
  29. Siegel MS and Graczykowski JW. 1991. Influence of porcine follicular fluid on the fertilizing capacity of human spermatozoa. Fertil. Steril., 55(6):1204-6 https://doi.org/10.1016/S0015-0282(16)54379-9
  30. Stock CE, Bates R, Lindsay KS, Edmonds DK and Fraser LR. 1989. Extended exposure to follicular fluid is required for significant stimulation of the acrosome reaction in human spermatozoa. J. Reprod. Fertil., 86(1):401-11 https://doi.org/10.1530/jrf.0.0860401
  31. Strehler E, Baccetti B, Sterzik K, Capitani S, Collodel G, De Santo M, Gambera L and Piomboni P. 1998. Detrimental effects of polyvinylpyrrolidone on the ultrastructure of spermatozoa (Notulae seminologicae 13). Hum. Reprod., 13(1):120-3 https://doi.org/10.1093/humrep/13.1.120
  32. Wittemer C, Ohl J, Bettahar-Lebugle K, Moreau L and Dellenbach P. 1996. Could in vitro fertilization with a modified sperm preparation technique be an option to micromanipulations? J. Assist. Reprod. Genet., 13(9):726-30 https://doi.org/10.1007/BF02066427
  33. World Health Organization. Laboratory for the examination of human semen and semen-cervical mucus interaction (4th ed.). Cambridge, Cambridge University Press. 1999
  34. Yao YQ, Ho PC and Yeung WS. 1999. Effects of human oviductal cell coculture on various functional parameters of human spermatozoa Fertil. Steril., 71(2):232-9 https://doi.org/10.1016/S0015-0282(98)00430-0
  35. Yao YQ, Yeung WS and Ho PC. 1996. Human follicular fluid inhibits the binding of human spermatozoa to zona pellucida in vitro. Hum. Reprod., 11(12):2674-80 https://doi.org/10.1093/oxfordjournals.humrep.a019190