Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Expression and Inheritance of bar Gene in Petunia hybrida Transformed with Agrobacterium

Petunia hybrida에 Agrobacterium으로 도입된 bar Gene의 발현과 후대검정

  • Ha, Young-Min (Department of Crops Biotechnology, Jinju National University) ;
  • Kim, Jong-Chul (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Sang-Woo (Department of Horticulture, Gyeongsang National University) ;
  • Lee, Shin-Woo (Department of Crops Biotechnology, Jinju National University) ;
  • Kim, Zhoo-Hyeon (Department of Horticulture, Gyeongsang National University)
  • 하영민 (진주산업대학교 작물생명과학과) ;
  • 김종철 (경상대학교 응용생명과학부) ;
  • 이상우 (경상대학교 원예학과) ;
  • 이신우 (진주산업대학교 작물생명과학과) ;
  • 김주현 (진주산업대학교 원예학과)
  • Published : 2003.06.01
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Abstract

This experiment was carried out to confirm the stability of bar gene introduced into petunia plant through Agrobacerium-mediated transformation. Twenty-five transgenic plants T$_{0}$ plants, back cross (BC$_1$) populations to wild type and F$_1$plants between different T$_{0}$ plants were prepared, and polymerase chain reaction(PCR), PCR-Southern blot analysis, and field test with 0.1% Basta treatment were done. The results of PCR, PCR-Southern blot hybridization, and field test indicated that NPTII and bar gene introduced into the genome of petuina plants were stably transmitted to their progenies, and conferred the plants resistance to herbicide, Basta.sta.

Keywords

References

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