Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Identification and Antibiotic Susceptibility Test of Bacteria from In Vitro Cultures of Pinellia ternata and Tea Plant

반하 및 차나무의 기내배양시 발생하는 세균의 동정 및 항생제 감수성 검정

  • Kim, Haeng-Hoon (National Institute of Agricultural Biotechnology, RDA) ;
  • Cho, Gyu-Taek (National Institute of Agricultural Biotechnology, RDA) ;
  • Yoon, Mun-Sup (National Institute of Agricultural Biotechnology, RDA) ;
  • Yoon, Ju-Won (National Institute of Agricultural Biotechnology, RDA) ;
  • Cho, Eun-Gi (National Institute of Agricultural Biotechnology, RDA)
  • 김행훈 (농업생명공학연구원 유전자원과) ;
  • 조규택 (농업생명공학연구원 유전자원과) ;
  • 윤문섭 (농업생명공학연구원 유전자원과) ;
  • 윤주원 (농업생명공학연구원 유전자원과) ;
  • 조은기 (농업생명공학연구원 유전자원과)
  • Published : 2003.06.01
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Abstract

Contamination of bacterial infection is one of serious problems in in vitro culture system of root crops. From the contaminated tubes over 140 of petiole cultures of Pinellia ternata, a medicinal plant, 4 genera 8species 48 strains of bacteria, including Aeromonas and Pseudomonas, were isolated and identified and another 8 strains were not fully identified. Most of them were motile Gram positive bacteria as in common in early stage of in vitro cultures. Six strains of bacteria, 5 of Gram negative, including Enterobacter, and 1 of Gram positive, were identified from the embryonic axes cultures of tea plant. From the susceptibility test to pre-screened 5 antibiotics, all of the bacteria except for 2 species of Pseudomonas were susceptible to cefotaxime 60∼100mg/L. While 60mg/L erythromycin only was effective to Pseudomonas. Combination of erythromycin 20mg/L and cefotaxime 60mg/L totally suppressed the growth of all bacterial strains tested. Susceptibility test of bacteria from tea embryonic axes cultures showed similar results. Combination of erythromycin 35mg/L and cefotaxime 60mg/L was effective to 15 bacterial strains and partially effective to 1 unidentified.

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References

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