Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimicrobial Activity of Rhus javanica Extracts Against Animal Husbandry Disease-Related Bacteria

가축질병 균주에 대한 오배자 추출물의 항균활성

  • Choi, Il (Dept. of Bioindustry and Technology, Sangji University)
  • 최일 (상지대학교 생명산업학과)
  • Published : 2003.12.01
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Abstract

Antimicrobial activity of Rhus javanica (RJ) extract against animal husbandry disease-related bacteria was determined by a paper disc method. The RJ extracts showed a significant antimicrobial activity against Gram positive (+) bacteria and especially the activity was most potent against L. monocytogenes and S. epidermidis. Minimum inhibitory concentrations (MIC) of the MeOH and EtOH extracts of RJ were in the range of 0.8 ∼ 16 mg/mL and 0.8 ∼ 10 mg/mL, respectively. Furthermore, among five solvent fractions (n-hexane, CHC1$_3$, EtOAc, n-BuOH and $H_2O$ frs.) from MeOH extract of RJ, the EtOAc fr. exhibited the most significant antimicrobial activity The antimicrobial activities of RJ extracts against most microbial strains were unstable by either heat treatment or acid treatment. The inhibitory effect of RJ extracts on microbial cell growth was further examined by the addition of 0, 100, 300, and 500 ppm of RJ extracts into growth medium. The growth of gram positive (+) bacteria, S. aureus, S. epidermidis and L. monocytogenes was inhibited for 72 hours when at least 300 ppm of RJ extracts added, but the growth of gram negative (-) bacteria was only inhibited when at least 500 ppm of RJ extracts were added. Taken together, tile antimicrobial activities of RJ extracts were more effective against gram positive (+) bacteria compared to those against gram negative (-) bacteria.

오배자 추출물을 제조하여 가축질병에 관련이 있는 균에 대한 항균활성을 조사하였다. 오배자 추출물은 그람양성균에 대해 높은 항균활성을 보였으며 그 중 S. epidermidis와 L. monocytogenes에서 가장 높은 항균활성을 보였다. 오배자 추출물의 최소저해농도는 MeOH추출물은 0.6 ∼ 12 mg/mL의 범위였으며 EtOH추출물은 0.2 ∼ 16 mg/mL 농도에서 항균활성을 보였고 오배자 분획물의 항균활성은 EtOAc층이 가장 높은 활성을 나타냈다. 열과 pH에 의한 항균활성 변화에 있어서는 대부분의 균주에서 항균력의 차이를 보였다. 오배자 추출물의 미생물 증식 억제 효과를 조사하기위해 증식 배지에 0, 100, 300 및 500 ppm의 추출물을 첨가하여 균주의 증식 억제효과를 조사하였다. 그람양성균인 S. aureus와 S. epidermidis는 300 ppm이상 첨가시 배양 후 72시간까지 증식이 정지된 상태를 보였으며 그람 음성균에서는500 ppm 이상 첨가시 증식이 저지되거나 저해효과가 있었다. 이런 결과를 종합하면 오배자 추출물은 그람 음성균에 비해 그람 양성균에 더욱 효과적인 항균활성이 있다고 보여진다.

Keywords

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