Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Purification and Characterization of Anti-Coagulant Activity Fraction from Persimmon Stem

감꼭지로부터 혈액응고저해물질의 정제와 특성

  • Published : 2003.12.01
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Abstract

Persimmon has been considered to have therapeutic values for various diseases in Korea. Dried persimmon has been applied to wounded parts for anti-inflammatory and analgesic activities. Anti-coagulant fraction from Persimmon stem was purified through gel filtration, phenyl Sepharose, DEAE-Sephadex and additional gel filtration column chromatographies. Its molecular weight was estimated to be 130,000 ∼ 180,000. By element analysis, its main components were C, H, and O. The anti -coagulant was heat- stable and completely inhibited after periodate oxidation, indicating that it was a complex carbohydrate.

감은 예전부터 민간약으로 종기나 염증질환, 치질에 곶감을 이겨 붙였고 부스럼이나 화상에는 불에 말린 감을 바르면 통증을 멎게 하고 새살을 돋게 하는 효능이 있고 고혈압을 예방하고 동맥경화에 효과가 있는 성분이 있다고 알려져 있다. 사람의 plasma에서 thrombin time(TT)를 사용한 혈액 응고저해 assay를 이용하여 감꼭지에서 혈액응고저해물질을 겔여과크로마토그래피, 소수성크로마토그래피, 음이온교환크로마토그래피, 또 한번의 겔여과크로마토그래피를 통해 정제했다. 감꼭지의 혈액응고저해물질은 분자량이 130,000 ∼ 180,000으로 거대 분자이고 구성분은 C, H, O로 구성되어 있고 periodate oxidation시 거의 활성이 소멸되고 열에 안정하며 주로 glucose와 galactose로 구성된 다당류로 추정된다.

Keywords

References

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