Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Screening for Antioxidative and Antimutagenic Capacities in 7 Common Vegetables Taken by Korean

한국인 상용채소 7종의 항산화능 및 항돌연변이능 검색

  • Published : 2003.12.01
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Abstract

This study was performed to investigate the antioxidative effect as the inhibition of malondialdehyde (MDA) and bovine serum albumin (BSA) conjugation reaction, inhibition of lipid peroxidation and the scavenging effect on 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, and antimutagenic capacities as the Ames test in 7 common vegetables taken by Korean for suggestion of prevention and dietetic treatment of chronic diseases and development of antioxidative and antimutagenic functional food. The water fractions of perilla leaves and sedum were most effective in the inhibition of MDA and BSA conjugation reaction showing 62.5% of inhibition rate among 7 vegetables. The inhibition rates of ethanol fractions of sedum and wild water dropwort on the lipid peroxidation were 67.1% and 61.5%, respectively. The ethanol fractions of crown daisy and wild water dropwort showed the most effective results among 7 vegetables in the DPPH radical scavenging capacities showing inhibition rate of 78.8% and 73.6%, respectively. The indirect and direct antimutagenic effects of ethanol extract of 7 vegetables were examined by Ames test using Salmonella typimurium TA98 and TA100. Inhibitory effects of wild water dropwort was superior to the other vegetables on the Ames test. These results suggest that common 7 vegetables taken by Korean are believed to be a possible antioxidative and antimutagenic capacities, although the results were different, more or less, according to the assay method and vegetables used.

한국인이 일상생활에서 손쉽게 구입하여 주로 상용하는 7종의 채소에 대해 MDA에 대한 단백질의 보호효과, linoleic acid를 이용한 지질과산화억제, DPPH 라디칼 소거 활성을 측정하여 항산화 효과를 측정하고, Ames test를 통한 항돌연변이능의 탐색 및 상호 비교 결과는 다음과 같다. 7종의 채소류물 추출물의 MDA에 대한 단백질의 보호효과에서는 깻잎과 돌나물(62.5%)이 가장 효과적으로 MDA의 교차결합을 차단하였고 부추, 참취, 돌미나리도 각각 57.4%, 53.5%, 45.6%의 저해율을 보였다. 또한 MDA의 교차결합의 형성 차단정도를 비교한 상대 저해율은 채소류의 물추출물 처리농도가 증가할수록 저해율이 높아짐을 알 수 있었다. 7종의 채소류 에탄올추출물의 지질과 산화억제 활성을 측정한 결과, 가장 우수한 저해활성을 보인 채소는 상대적으로 돌나물(67.1%), 돌미나리(61.5%)가 유지류의 자동산화 반응에 대한 항산화효과가 가장 높았다. 7종의 채소류 에탄올추출물의 DPPH 라디칼 소거 활성을 측정한 결과, 쑥갓(78.8%), 돌미나리 (73.6%)가 높은 저해활성을 보였다. 각 채소류 에탄올 추출물들의 $IC_{50}$/값은 쑥갓이 31.1 $\mu\textrm{g}$/assay, 돌미나리가 93.8 $\mu\textrm{g}$/assay이었다. Ames test를 통한 채소류 에탄올 추출물의 항돌연변이능 실험에서는, 간접 작용 항돌연변이 능을 S. typimurium TA98로 측정한 결과 2-anthramine에 대한 항돌연변이능은 돌미나리(99.8%), 깻잎(85.6%), 부추(79.6%), 쑥갓(79.4%), 시금치(74.5%)등이 높았다. S. typimurium TA100에서 2-anthramine의 돌연변이능에 대한 저해효과는 돌미나리(100.0%), 쑥갓(79.1%), 시금치 (71.3%) 등이 높았다. 직접작용 항돌연변이능을 S. typimurium TA98로 실험한 결과 2-nitrofluorene의 돌연변이능의 저해율이 높은 채소는 깻잎(82.8%), 참취 (82.3%), 쑥갓(56.6%)이었다. 또한 S. typimurium TA100 에서 sodium azide의 돌연변이능에 저해효과가 가장 큰 채소는 돌미나리 (91.8%)이었다. 따라서 MDA & BSA conjugation 반응, 지질과산화억제활성, DPPH 라디칼 소거 활성을 측정한 결과, 각 측정법에 따라 항산화효과에 차이가 있었으나 이들 7종의 채소류를 상용함으로써 광범위하게는 만성 성인병의 예방 및 치료에 효과를 볼 수 있을 것이라고 사료되며, 본 연구의 결과가 항산화성 및 항돌연변이성 기능성 식품을 개발할 수 있는 기초자료로 이용될 수 있으리라 사료된다.

Keywords

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