Production of Laccase and Bioremediation of Pentachlorophenol by Wood-Degrading Fungus Trichophyton sp. LKY-7 immobilized in Ca-Alginate Beads

  • Hyunchae Jung (College of Agriculture and Life Science, Sunchon National University) ;
  • Kyuhwan Hyun (College of Agriculture and Life Science, Sunchon National University) ;
  • Park, Chongyawl (Division of Forest Science, Gyeongsang National University)
  • Published : 2003.12.01

Abstract

The wood-degrading fungus Trichophyton sp. LKY-7 (T. LKY-7) was immobilized in ca-alginate beads for laccase production and PCP bioremediation. The immobilized T. LKY-7 enabled the repeated use of this fungus for laccase production and produced high amount of laccase throughout 5 cycles incubation. As a laccase inducer. oak wood meal (Quercus variabilis) seemed to be effective laccase inducer for T. LKY-7, and the optimum addition amount was 1% (W/W) in glucose-peptone medium. Bioremediation of pentachlorophenol by the immobilized T. LKY-7 reached an efficency of up to 90% without toxic inhibition. The immobilized T. LKY-7 might thus be applicable for semicontinuous laccase production and bioremediation to serve inoculum for reactor system.

Keywords

References

  1. Eriksson,K.EL.;lanchette,R.A.;Ander,P.
  2. Appl. Environ. Microbiol v.56 Lamar,R.T.;Dietrich,D.M.
  3. Water Res. v.29 Alleman,B.C.;Logan,B.E.;Gilbertson,R.I. https://doi.org/10.1016/0043-1354(94)E0106-G
  4. Biotech. Bioeng. v.35 Mileski,G.J.;Bumpus,J.A.;Jurek,M.A.;Aust S.D. https://doi.org/10.1002/bit.260351108
  5. Enzyme Microb. Technol. v.13 Roy-Arcand,L;Archibald F.S. https://doi.org/10.1016/0141-0229(91)90128-W
  6. Appl. Microbiol. Biotechnol. v.53 Ullah,M.A.;Bedford,C.T.;Evans,C.S. https://doi.org/10.1007/s002530050013
  7. Enzyme Microb. Technol. v.8 Shuttleworth,K.L.;Bollag J.M. https://doi.org/10.1016/0141-0229(86)90108-0
  8. Appl. Microbiol. Biotechnol. v.54 Ullah,M.A.;Kadhim,H.R.;Rastall,A.;Evans,C.S. https://doi.org/10.1007/s002530000466
  9. Enzyme Microb. Technol. v.30 Jung,H.C.;Xu,F.;Li,K. https://doi.org/10.1016/S0141-0229(01)00485-9
  10. Arch. Microbiol. v.85 Kirk,T.K.;Schultz,E.;Connors,W.J.;Lorenz,L.F;Zeikus,J.G.
  11. Appl. Environ. Microbiol v.62 Eggert,C.;Temp,U.;Eriksson,K.EL.
  12. Bioprocess engineering v.21 Leidig,E.;Prusse,U.;Vorlop,K.D.;Winter,J.
  13. v.59 Ricotta,A.;Unz,R.F.;Borag,J.M.
  14. Appl. Environ. Microbiol. v.62 Ruttimann,J.C.;Lamar,R.T.
  15. Arch. Environ. Contam. Toxicol. v.19 Dec,J.;Bollag,J.M. https://doi.org/10.1007/BF01059073