The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Erwinia pyrifoliae, a Causal Endemic Pathogen of Shoot Blight of Asian Pear Tree in Korea

  • Shrestha, Rosemary (Division of Biological Environment, Kangwon National University) ;
  • Koo, Jun-Hak (Division of Biological Environment, Kangwon National University) ;
  • Park, Duck-Hwan (Division of Biological Environment, Kangwon National University) ;
  • Hwang, In-Gyu (School of Agricultural Biotechnology, Seoul National University) ;
  • Hur, Jang-Hyun (Division of Biological Environment, Kangwon National University) ;
  • Lim, Chun-Keun (Division of Biological Environment, Kangwon National University)
  • Published : 2003.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Bacterial strains were isolated from diseased samples of shoot blight collected from different pear growing orchards of Chuncheon, Korea from 1995 to 1998. Forty-nine strains showed their pathogenicity on immature fruit and shoot of pear. Microbiological, physiological, and biochemical tests were performed on these pathogenic strains. One strain, designated as WT3 in this study, was selected as a representative strain as it was collected from the first outbreak area in Jichonri, Chuncheon in 1995. Further detailed characterization of the strain WT3 was done by PCR amplification using specific primers described previously for distinguishing Erwinia pyrifoliae from its close pathogen Erwinia amylovora. Based on phenotypical, biochemical, and molecular analyses, strain WT3 was identified as a shoot blight pathogen which was the same as E. pyrifoliae Ep16 previously described by a German group in 1999.

Keywords

References

  1. Bereswill, S., Pahl, A., Bellemann, P., Zeller, W. and Geider, K. 1992. Sensitive and species-specific detection of Erwinia amylovora by polymerase chain reaction analysis. Appl. Environ. Microbiol. 58:3522-3526
  2. Bereswill, S., Jock, S., Bellemann, P. and Geider, K 1998. Identification of Erwinia amylovora by growth in the presence of copper sulfate and by capsule staining with lectin. Plant Dis. 82:158-164 https://doi.org/10.1094/PDIS.1998.82.2.158
  3. Falkenstein, H., Zeller, W. and Geider, W. 1989. The 29 kb plasmid common in strains of Erwinia amylovora, modulates development of fire blight symptoms. J. Gen. Microbiol. 135:2643-2650
  4. Keane, P. J., Kerr, A and New, P. B. 1970. Crown gall of stone fruit. II. Identification and nomenclature of Agribacterium isolates. Aust. J. Biol. Sci. 23:85-595
  5. Kim, W-S., Hildebrand, M., Jock, S. and Geider, K. 2001a. Molecular comparison of pathogenic bacteria from pear treesin Japan and the fire blight pathogen Erwinia amylovora. Microbiology 147:2951-2959 https://doi.org/10.1099/00221287-147-11-2951
  6. Kim, W-S., Jock, S., Paulin, J.-P., Rhim, S.-L. and Geider, K. 2001b. Molecular detection and differentiation of Erwinia pyrifoliae and host range analysis of the Asian pear pathogen. Plant Dis. 85:1183-1188 https://doi.org/10.1094/PDIS.2001.85.11.1183
  7. Kim, W.-S., Garden, L., Rhim, S.-L. and Geider, K. 1999. Erwinia pyrifoliae sp. nov., a novel pathogen that affects Asian pear trees (Pyrus pyrifolia Nakai). Intl. J. Sys. Bacteriol. 49:899-906 https://doi.org/10.1099/00207713-49-2-899
  8. McGhee, G. C, Schnabel, E. L., Maxson-Stein, K, Jones, B., Stromberg, V. K, Lacy, G. H. and Jones, A. L. 2002. Relatedness of chromosomal and plasmid DNAs of Erwinia pyrifoliae and Erwinia amylovora. Appl. Environ. Microbiol. 63:4421-4426
  9. Nakagawa, T., Shimada, M., Mukai, H., Asada, K., Kato, I., Fujino, K. and Sato, T. 1994. Detection of alcohol-tolerant Hiochi bacteria by PCR. Appl. Environ. Microbiol. 60:637-640
  10. Perry, S. F. 1995. Freeze drying and cryopreservation of bacteria. pp. 21-30. In: Methods in molecular biology. J. G. Day and M. R. McLellan (eds.) Humana Press Inc
  11. Rhim, S.-L., Voelkisch, B., Gardan, L., Paulin, J.-P., Langlotz, C., Kim, W.-S. and Geider, K. 1999. An Erwinia species, different from E. amylovora cause a necrotic disease of Asian pear trees. Plant Pathol. 48:514-520 https://doi.org/10.1046/j.1365-3059.1999.00376.x
  12. Sambrook, J. and Russell, D. W. 2001. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor, NY, U.S.A
  13. Schaad, N. W., Jones, J. B. and Chun, W. 1988. Laboratory guide for identification of plant pathogenic bacteria. 2$^n^d$ ed. Amer. Phytopath. SOC., MN, USA
  14. Shrestha, R, Hur, J. H. and Lim, C. K 2001. The effects of temperature and pH on the growth of Asian pear pathogen, Erwinia pyrifoliae. (abstr.) Phytopathology. 91(suppl.):S82
  15. Van der Zwet T. and Keil, H. L. 1979. Fire blight: a bacterial disease of rosaceous plants. Agriculture Handbook No. 510, US Government printing office, Washington, DC
  16. Weisburg, W. G., Barns, S. M., Pelletier, D. A. and Lane, D. J. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173:697-703

Cited by

  1. Diversity and detection of Korean Erwinia pyrifoliae strains as determined by plasmid profiling, phylogenetic analysis and PCR vol.56, pp.6, 2007, https://doi.org/10.1111/j.1365-3059.2007.01679.x
  2. Erwinia spp. from pome fruit trees: similarities and differences among pathogenic and non-pathogenic species vol.26, pp.1, 2012, https://doi.org/10.1007/s00468-011-0644-9
  3. Detection of Erwinia species from the apple and pear flora by mass spectroscopy of whole cells and with novel PCR primers vol.112, pp.1, 2012, https://doi.org/10.1111/j.1365-2672.2011.05165.x
  4. Associations between Ectomycorrhizal Fungi and Bacterial Needle Endophytes in Pinus radiata: Implications for Biotic Selection of Microbial Communities vol.7, 2016, https://doi.org/10.3389/fmicb.2016.00399
  5. Genetic islands in pome fruit pathogenic and non-pathogenic Erwinia species and related plasmids vol.6, 2015, https://doi.org/10.3389/fmicb.2015.00874
  6. Classification and Taxonomy of Vegetable Macergens vol.6, 2015, https://doi.org/10.3389/fmicb.2015.01361
  7. Phenotypic and genetic characterization of Erwinia rhapontici isolated from diseased Asian pear fruit trees vol.40, pp.5, 2012, https://doi.org/10.1007/s12600-012-0251-3
  8. Functions and origin of plasmids in Erwinia species that are pathogenic to or epiphytically associated with pome fruit trees vol.26, pp.1, 2012, https://doi.org/10.1007/s00468-011-0630-2
  9. Comparative genomics of JapaneseErwinia pyrifoliaestrain Ejp617 with closely related erwinias vol.56, pp.2, 2013, https://doi.org/10.1139/gen-2012-0094