Korean Journal of Animal Reproduction (한국가축번식학회지)

The Korean Society of Animal Reproduction (한국동물번식학회)
권호 표지

Cellulose Digestibility Increased with CelD Transgenic Pigs

  • Park, J.K. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Lee, Y.K. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Lee, P.Y. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Kim, S.W. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Kim, S.J. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Lee, H.G. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Han, J.H. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Chung, H.K. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Park, C.G. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Chung, Y.H. (Division of Animal Biotechnology, National Livestock Research Institute, RDA) ;
  • Lee, C.H. (Department of Animal Scienc, Korea National Agricultural College, RDA) ;
  • Lee, H.T. (Animal Resources Research Center, Konkuk University) ;
  • Chang, W.K. (Division of Animal Biotechnology, National Livestock Research Institute, RDA)
  • Published : 2003.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to test the cellulose digestibility using the transgenic pigs harboring cellulose degradation gene D (CelD). After delivered offsprings between normal pig and transgenic swine, DNA was isolated from piglets tail for PCR analysis. In first generation, five out of 65 piglets showed CelD positive. Unfortunately, four CelD-positive pigs were died during growing, but one survived pig was used as a transgenic founder to produce F$_1$ descendents. Among 3 F$_1$ transgenic pigs produced, one died and the remaining two pigs were used to test the fiber digest efficiency. An assorted feed was composite of 5％ fiber with other ingredients. The feed of 3 kg per day was provided to the pigs including transgenic founders and littermate controls. The manure quantity was measured daily for a month, and all manures were dried for three days to analysis nitrogen, phosphate and fiber concentrations. The fiber digestion efficiencies of the transgenic F$_1$ pigs showed approximately 10％ higher than those of control pigs. Fiber digestion was not greatly improved in transgenic pigs as it had been expected approximately 30％. Nitrogen concentration of transgenic pig's manure was slowly decreased compare to the control pigs. Because there were only two transgenic pigs tested, a large number of transgenic pigs may be necessary to obtain more reliable data. Breeding of animals to obtain sufficient transgenic pigs subjected for a further study is on progress. Taken together, this study demonstrated successful production of transgenic pigs with increase of cellulose digestibility in the porcine feed.

Keywords

References

  1. Bayer, E. A., Morag, E., Shoham, Y., Tormo, J. and Lamed, R. 1996. The cellulosome: a cell surface organelle for the adhesion to and degradation of cellulose. p. 155-182. In M. Fletcher (ed.). Bacterial adhesion: molecular and ecological diversity. Wiley-Liss. Inc. New York. N.Y.
  2. Beguin, P. 1990. Molecular biology of cellulose degradation. Annu. Rev. Microbiol. 44:219-248 https://doi.org/10.1146/annurev.mi.44.100190.001251
  3. Beguin, P. and Lemaire, M. 1996. The cellulosome: an exocellular, multiprotein complex specialized in cellulose degradation. Crit. Rev. Biochem. Mol. BioI. 31:201-236.
  4. Beguin, P., Millet, J. and Aubert, J. P. 1987. The cloned cel(cellulose degradation) genes of Clostridium thermocellum and their products. Microbiol. Sci. 4:277-280
  5. Belaich, J. P., Tardif, C., Belaich, A. and Gaudin, C. 1997. The cellulolytic system of Clostridium cellulolyticum. J. Biotechnol. 57:3-14
  6. Brinster, R. L., Allen, J. M., Behringer, R. R., Gelinas, R. E. and Palmiter, R. D. 1988. Introns increase transcriptional dfficiency in transgenic mice. Proc. Natl. Acad. Sci. USA. 85:836-40 https://doi.org/10.1073/pnas.85.3.836
  7. Doi, R. H., Goldstein, M., Hashida, S., Park, J. S. and Takagi, M. 1994. The Clostridium cellulovorans cellulosome. Crit. Rev. MicrobioI. 20:87-93
  8. Hammer, R. E., Pursel, V. G., Rexroad, C. E., Wall, R. J., Bolt, D. J., Ebert, K. M., Palmiter, R. D. and Brinster, R. L. 1985. Production of trans genic rabbits, sheep and pigs by microinjection. Nature. 315:680-683 https://doi.org/10.1038/315680a0
  9. Martin, M. J. and Pinkert, C. A. 1994. Production of transgenic swine. In: Pinkert, C.A. (ed) Transgenic Animal Technology, Academic Press, San Diego. pp. 118-125
  10. Morage, E., Yaron, S., Lamed, R., Kenig, R, Shoham, Y. and Bayer, E. A. 1996. Dissociation of the cellulosome of Clostridium thermocellum under nondenaturing conditions. J. Biotechnol. 51:235-242 https://doi.org/10.1016/S0168-1656(96)01601-X
  11. Nottle, M. B., Haskard, K. A., Verma, P. J., Du, Z. T., Grupen, C. G., et al. 2001. Effect of DNA concentration on transgenesis rates in mice and pigs. Transgenic Res. 10:523-531 https://doi.org/10.1023/A:1013007329936
  12. Padfield, P. J. 2000. Control of the priming and triggering phase of exocytosis in the pancreatic acinar cell. J. Korean Med. Sci. 15:49-50 https://doi.org/10.3346/jkms.2000.15.1.49
  13. Park, J. K., Lee, Y. K., et aI., 2002. Production of transgenic pig harboring the cellulase digest gene(CeID). Korean J. Animal Reprod. 26:87-94
  14. Pursel, V. G., Hammer, R G., Bolt, D. J., Palmiter, R. D., and Brinster, R. L. 1990a. Integration, expression and germ-line transmission of growth-related genes in pigs. J. Reprod. Fertil. 41:77-87
  15. Teunissen, M. J. and Op den Camp, H. J. M. 1993. Anaerobic fungi and their cellulolytic and xylanolytic enzymes. Antonie Leeuwenhoek Int. J. Genet. Mol. Microbiol. 63:63-76
  16. Williams, J. A., Groblewski, G. E., Ohnishi, H. and Yule, D. J. 1997. Stimulus secretion coupling of pancreatic digestive enzyme secretion. Digestion. 58:42-45 https://doi.org/10.1159/000201524