Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effects of Mitochondrial DNA Polymorphism on Growth Traits of Hanwoo

mt DNA 다형이 한우 성장에 미치는 영향

  • Jeon, G.J. (National Livestock Research Institute, R.D.A) ;
  • Chung, H.Y. (National Livestock Research Institute, R.D.A) ;
  • Choi, J.G. (National Livestock Research Institute, R.D.A) ;
  • Lee, M.S. (National Livestock Research Institute, R.D.A) ;
  • Chung, Y.H. (National Livestock Research Institute, R.D.A) ;
  • Lee, C.W. (National Livestock Research Institute, R.D.A) ;
  • Park, J.J. (National Livestock Research Institute, R.D.A) ;
  • Ha, J.M. (National Livestock Research Institute, R.D.A) ;
  • Lee, H.K. (Hankyong National University) ;
  • Na, K.J. (National Livestock Research Institute, R.D.A)
  • Published : 2003.12.01
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Abstract

한우의 mt DNA cytochrome oxidase subunit I, II, 및 III complex지역의 유전적 다형현상을 제한효소를 이용하여 검출하였다. PCR primer 6종에 대하여 20가지 제한효소를 처리하였으며, Pst I, Pvu II, Rsa I, Eco RI, Bgl II, and Msp I 제한효소를 사용하여 유전적 변이를 검출하였다. 검출된 변이체와 한우의 성장과의 관련성을 조사한 결과 cytochrome oxidase subunit III complex 지역의 유전염기서열을 근거로 제작한 primer Mt9 좌위에서 제한효소 PvuII를 이용한 절단형과 체중형질 인 WT15(P<0.05) 및 WT18(P<0.01)에서 고도의 유의성이 관찰되었다. 아울러 , Mt9-Pvu II(P=0.07), Mt6-Bgl II(P=0.05), and Mt8-Rsa I(P=0.05) 좌위 또한 WT9, WTl5, and WT15에서 각각 통계적 유의성이 관찰되었다. 따라서 본 결과는 cytochrome oxidase subunit III complex segments가 candidate gene으로서 기초적 유전정 보 제공은 물론 유전적 개량을 위해 사용될 수 있을 것으로 사료된다.

Keywords

References

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