Effects of Citrus Essential Oils on Melanin Production in B16 Melanoma Cells

B16 Melanoma 세포에서 Citrus Essential Oil이 Melanin 생성에 미치는 영향

  • 임혜원 (중앙대학교 의약식품대학원) ;
  • 조남영 (중앙대학교 의약식품대학원) ;
  • 윤미연 (중앙대학교 의약식품대학원) ;
  • 차상복 (중앙대학교 의약식품대학원) ;
  • 김경원 (중앙대학교 의약식품대학원) ;
  • 박영미 (중앙대학교 의약식품대학원) ;
  • 이지윤 (중앙대학교 약학대학) ;
  • 이진희 (중앙대학교 약학대학) ;
  • 김창종 (중앙대학교 약학대학)
  • Published : 2003.02.01

Abstract

This study is performed to investigate the effects of citrus essential oils on melanin production in B16 melanoma cells. Five kinds of citrus essential oils (Bergamot, Grapefruit, Lemon, Mandarin, Petigrain) did not have any influence on DPPH radical scavenger activity, cell growth and cytotoxicity in B16 melanoma cells. Both mandarin and petigrain essential oils dose-dependently inhibited purified tyrosinase activity, but bergamot did not. In 1$\mu$M MSH-stimulated B16 melanoma cells, all of 5 citrus essential oils inhibited melanin production in a dose dependent manner. From the above results, it is possible that citrus essential oils may be developed to be an anti-melanogenesis agent on the basis of their inhibitory effect on MSH-induced melanin production.

References

  1. FASEB J. v.5 Enzymatic control of pigmentation in mammals Hearing, V. J.;Tsukamoto, K. https://doi.org/10.1096/fasebj.5.14.1752358
  2. Planta Med. v.59 Inhibition of tyrosinase activity by cultured lichen tissues and bionts Higuchi,M.;Miura, Y.;Boohena, J.;Kinoshita, Y.;Yamamamoto, Y.;Yushimura, I.;Yamaha, Y. https://doi.org/10.1055/s-2006-959662
  3. Yakhak Hoeji. v.42 Antioxidative effects of the rhizomo of Rhodiola sachalinensis Ryu, K. Y.;Kang, W. S.;Kim, Y. H.;Jang, H. D.;Hong, J.T.;Yoo, H. S.;Yun, Y. P.
  4. Yakhak Hoeji v.42 Tyrosinase inhibition activity of some herbal drugs Park, J. H.;Shin, Y. G.;Shin, U. K.;Baek, S. K.;Lee, S.;K, Hee.;Chung, M. H.;Park, Y. I.
  5. Yakhak Hoeji v.42 Insolation of Inhibitory components on tyrosinase activity from the vark of paeonia moutan Lee, S. H.;Park, J. S.;Kim, S. Y.;Kim, J. J.;Chung, S. R.
  6. The art of Aromatherapy Tisserand, R.
  7. Planta Med. v.62 Effect of some components of the essential oil of chamomile, Chamomilla recutita, on histamine release from rat mast cells Miller, T.;Wittstock, U.;Lindequist, U.;Teuscher, E. https://doi.org/10.1055/s-2006-957799
  8. Eur, J. Pharmacol. v.23 Mast cell involvement in the rat paw oedema response to 1,8-cineole, the main constituent of eucalyptus and rosemary oils Santos, F. A.;Rao, V. S.
  9. J. Agric Food Chem. v.48 Radicalscavenging activites of citrus essential oils and their componenrs; detection using 1,1-diphenyl-2-picrylhydrazyl Choi, H. S.;Song, H. S.;Ukeda, H.;Sawamura, M.
  10. Phytother Res. v.14 Antioxidant activity of Nigekka sativa essential oil Burits, M.;Bucar, F. https://doi.org/10.1002/1099-1573(200008)14:5<323::AID-PTR621>3.0.CO;2-Q
  11. Arzneimittelforschung v.50 no.135 Antioxidant properties of essential oils. Possible explanations for their anti-inflammatory effects Grassmann, J.;Hippeli, S.;Dornisch, K.;Rohnert, U.;Beuscher, n.;Elstner, E. F.
  12. J. Dermatol. v.25 Efficacy of topical treatment of pigmentation skin disorders with plant hydroqyinone glucoskdes as assessed by quantitatibe color analysis Clarys, P.;Barel, A. https://doi.org/10.1111/j.1346-8138.1998.tb02425.x
  13. Arch. Biochem. Biophys. v.15 Purification of hamster melanoma tyrosinases and structural studies of their asparagine-linked sugar chains Ohkura, T.;Yamashita, K.;Mishima, Y.;Kobata, A.
  14. J. Pharmacol. Exp. Ther. v.276 Arbutin mechanism of its depigmenting action in human melanocyte culture Maeda, K.;Fukuda, M.
  15. J. Appl. Toxicol. v.21 Kojic acid reduces the cytotoxic effects of sulfur mustard on cultures containing human melanoma cells in vitro Smith, C. N.;Lindsay, C. D. https://doi.org/10.1002/jat.777