Effects of Citrus Essential Oils on Melanin Production in B16 Melanoma Cells

B16 Melanoma 세포에서 Citrus Essential Oil이 Melanin 생성에 미치는 영향

  • 임혜원 (중앙대학교 의약식품대학원) ;
  • 조남영 (중앙대학교 의약식품대학원) ;
  • 윤미연 (중앙대학교 의약식품대학원) ;
  • 차상복 (중앙대학교 의약식품대학원) ;
  • 김경원 (중앙대학교 의약식품대학원) ;
  • 박영미 (중앙대학교 의약식품대학원) ;
  • 이지윤 (중앙대학교 약학대학) ;
  • 이진희 (중앙대학교 약학대학) ;
  • 김창종 (중앙대학교 약학대학)
  • Published : 2003.02.01


This study is performed to investigate the effects of citrus essential oils on melanin production in B16 melanoma cells. Five kinds of citrus essential oils (Bergamot, Grapefruit, Lemon, Mandarin, Petigrain) did not have any influence on DPPH radical scavenger activity, cell growth and cytotoxicity in B16 melanoma cells. Both mandarin and petigrain essential oils dose-dependently inhibited purified tyrosinase activity, but bergamot did not. In 1$\mu$M MSH-stimulated B16 melanoma cells, all of 5 citrus essential oils inhibited melanin production in a dose dependent manner. From the above results, it is possible that citrus essential oils may be developed to be an anti-melanogenesis agent on the basis of their inhibitory effect on MSH-induced melanin production.


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