YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effects of Protoberberine Alkaloids on L-DOPA-Induced Cytotoxicity in PC12 Cells

Protoberberine 알칼로이드가 PC12 세포중의 L-DOPA 유도 세포독성 작용에 미치는 영향

  • 이재준 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 김유미 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 김춘매 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 양유정 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 강민희 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 이명구 (충북대학교 약학대학, 생물건강산업개발연구센터)
  • Published : 2003.08.01
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Abstract

Previously, protoberberine alkaloids such as berberine and palmatine have been found to lower dopamine content in PC12 cells (Shin et at., 2000). In this study, the effects of berberine and palmatine on L-DOPA-induced increase in dopamine level and cytotoxicity in PC12 cells were investigated. Treatment of PC12 with L-DOPA at concentration ranges of 20∼50 $\mu$M increased dopamine content and the increase in dopamine levels by L-DOPA was inhibited by 10∼40 $\mu$M berberine and 10∼80 $\mu$M palmatine, which the concentration ranges did not show a cytotoxicity. However, berberine and palmatine at concentrations higher than 50 $\mu$M and 100 $\mu$M caused a cytotoxicity, respectively. In addition, berberine (10∼20 $\mu$M) and palmatine (10∼50 $\mu$M) at non-cytotoxic concentration ranges aggravated L-DOPA-induced cytotoxicity in PC12 cells (L-DOPA concentration ranges, 20∼50 $\mu$M). The L-DOPA-induced cytotoxicity was also significantly potentiated by berberine (50 $\mu$M) and palmatine (100 $\mu$M) with cytotoxic ranges. These data demonstrate that berberine and palmatine inhibit L-DOPA-induced increase in dopamine content and stimulate L-DOPA-induced neurotoxicity. Therefore, the possibility that the long-term L-DOPA treated patients with berberine and palmatine could be checked the adverse symptoms.

Keywords

References

  1. Chinese drugs of plant origin Tang, W.;Eisenbrand, G.
  2. J. Ethnopharmacol. v.15 Comparative in vitro antimicrobial activity of chinese medicinal herbs Franzblau, S. G.;Cross, C. https://doi.org/10.1016/0378-8741(86)90166-2
  3. Am. J. Chin. Med. v.14 Two-year experience with :San-Huang-Hsih-Tang" in essential hypertension Chen, H. G.;Hsieh, M. T. https://doi.org/10.1142/S0192415X86000090
  4. Jpn. J. Pharmacol. v.51 A possible mechanism for the gastric mucosal protection by OREN-GEDOKU-TO (OGT), A traditional herbal medicine Takase, H.;Imanishi, K.;Miura, O.;Yumioka, E.
  5. Nippon Yakurigaku Zasshi v.72 Behavioral pharmacology of berberine-type alkaloids (1), Central depressive action of Coptis Rhizoma and its constituents Yamahara, J. https://doi.org/10.1254/fpj.72.899
  6. Arzneim.-Forsch./Drug Res. v.42 Evaluation of the α-adrenoceptor antagonistic action of berberine in isolated organs Oelmez, E.;Ilhan, M.
  7. Neurochem. Res. v.25 Inhibition of dopamine biosynthesis by protoberberine alkaloids in PC12 cells Shin, J. S.;Kim, E. I.;Kai, M.;Lee, M. K. https://doi.org/10.1023/A:1007541020736
  8. Arch. Pharm. Res. v.20 Inhibitory effects of noscapine on dopamine biosynthesis in PC12 cells Shin, J. S.;Lee, S. S.;Lee, M. K. https://doi.org/10.1007/BF02973950
  9. Med. Sci. Res. v.24 Inhibition of tyrosine hydroxylase by berberine Lee, M. K.;Zhang, Y. H.
  10. Arch. Pharm. Res. v.19 Inhibition of tyrosine hydroxylase by palmatine Lee, M. K.;Zhang, Y. H.;Kim, H. S. https://doi.org/10.1007/BF02976236
  11. Med. Sci. Res. v.25 Inhibition of tyrosine hydroxylase by hydrastine Lee, M. K.;Zhang, Y. H.;Shin, J. S.;Lee, S. S.
  12. Biochem. Pharmacol. v.56 Isoquino-line derivatives as endogenous neurotoxins in the aetiology of Parkinsons disease McNaught, K. St. P.;Carrupt, P. A.;Altmore, C.;Cellamare, S.;Carotti, A.;Testa, B.;Jenner, P.;Marsden, C. D. https://doi.org/10.1016/S0006-2952(98)00142-7
  13. Biochem. Pharmacol. Tetrahydropapaveroline aggravates L-DOPA-induced neurotoxicity in PC12 cells Lee, J. J.;Kim, Y. M.;Yin, S. Y.;Park, H. D.;Kang, M. H.;Hong, J. T.;Lee, M. K.
  14. Free Rad. Biol. Med. v.30 Oxidative DNA damage and cytotoxicity induced by copper-stimulated redox cycling of salsolinol, a neurotoxic tetrahydroisoquinoline alkaloid Jung, Y. J.;Surh, Y. J. https://doi.org/10.1016/S0891-5849(01)00548-2
  15. Neurochem. Int. v.25 1-Methyl-4-phenyl-pyridium (MPP+)-induced cell death in PC12 cells: inhibitory effects of several drugs Itano, Y.;Kitamura, Y.;Nomura, Y. https://doi.org/10.1016/0197-0186(94)90017-5
  16. J. Neurochem. v.64 L-DOPA cytotoxicity to PC12 cells in culture is via its autoxidation Basma, A. N.;Morris, E. J.;Nicklas, W. J.;Geller, H. M. https://doi.org/10.1046/j.1471-4159.1995.64020825.x
  17. J. Neurochem. v.30 Short-term regulation of catecholamine biosynthesis in nerve growth factor responsive clonal line of rat pheochromocytoma cells Greene, L. A.;Rein, G.
  18. Advance in Cellular Neurobiology v.3 PC12 pheochromocytoma cultures in neurobiological research Greene, L. A.;Tischler, A. S.
  19. J. Chromatogr. v.344 High-performance liquid chromatography of plasma catecholamines using 1,2-dirivatization reagent Mitsui, A.;Nohta, H.;Ohkura, Y.
  20. J. Chromatogr. v.378 Occurrence of aromatic L-amino acid decarboxylase in human plasma and its assay by high-performance liquid chromatography with fluorescence detection Lee, M. K.;Nohta, H.;Ohkura, Y. https://doi.org/10.1016/S0378-4347(00)80729-X
  21. J. Immunol. Meth. v.65 Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays Mosmann, T. https://doi.org/10.1016/0022-1759(83)90303-4
  22. J. Biol. Chem. v.193 Protein measurement with the Folin phenol reagent Lowry, O. H.;Rosenbrough, N. L.;Farr, A. L.;Randall, R. L.
  23. J. Neurochem. v.73 Enhancing effect of manganese on L-DOPA-induced apoptosis in PC12 cells: role of oxidative stress Migheli, R.;Godani, C.;Bciola, L.;Delodu, M. R.;Serra, P. A.;Zangani, D.;Natale, G. D.;Miele, E.;Desole, M. S. https://doi.org/10.1046/j.1471-4159.1999.0731155.x