Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Antioxidant Activity from the Stem Bark of Albizzia julibrissin

  • Jung, Mee-Jung (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Chung, Hae-Young (College of Pharmacy, Pusan National University) ;
  • Kang, Sam-Sik (Natural Products Research Institute, Seoul National University) ;
  • Choi, Jin-Ho (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Bae, Kae-sun (College of Natural Resources and Life Science, Dong-A University) ;
  • Choi, Jae-Sue (Faculty of Food Science and Biotechnology, Pukyong National University)
  • Published : 2003.06.01
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Abstract

The antioxidant activity of the stem bark from Albizzia julibrissin was evaluated for its potential to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, to inhibit the generation of the hydroxyl radical ($\cdot OH$), total reactive oxygen species (ROS) and to scavenge authentic peroxynitrites ($ONOO^{-}$). The methanol extract of A. julibrissin exhibited strong antioxidant activity in the tested model systems. Therefore, it was further fractionated using several solvents. The antioxidant activity of the individual fractions were in the order of ethyl acetate (EtOAc) ＞ n-butanol (n-BuOH) ＞ dichloromethane ($CH_2 CI-2$) ＞ and water ($H_2O$). The ethyl acetate soluble fraction, which exhibited strong antioxidant activity, was further purified by repeated silicagel, Sephadex LH-20 and RP-18 gel column chromatography. Sulfuretin (1) and 3 ,4 ,7-trihydroxyflavone (2) were isolated as the active principles. Compounds 1 and 2 exhibited good activity in all tested model systems. Compound 1 exhibited five times more inhibitory activity on the total ROS than Trolox. Compound 2 showed six times stronger DPPH radical scavenging activity than L-ascorbic acid. These results show the possible antioxidant activity of the A. julibrissin crude extract and its major constituents.

Keywords

References

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