약학회지 (YAKHAK HOEJI)

  • 제47권6호
  • /
  • Pages.438-443
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  • 2003
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  • 0377-9556(pISSN)
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  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

페튜인에 대한 면역글로부린 Y의 생성 및 친화성 컬럼에 의한 분리

Production of Anti-fetuin Immunoglobulin Y and its Isolation Using an Affinity Column

  • 발행 : 2003.12.01
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초록

For the investigations of the usefulness of immunoglobulin Y (IgY), we attempted to produce and isolate IgY from egg yolk of white Leghorn hens immunized with fetuin as a model antigen. We used three methods to optimize the recovery of IgY: water dilution, polyethylene glycol, and carrageenan. The daily yield of anti-fetuin IgY from egg yolk was also examined, and it was isolated using a fetuin-affinity column at a yield of IgY of 2.7% from total IgY Furthermore, peroxidase-labeled antifetuin IgY was prepared, and was used to determine the minimum sensitivity against fetuin, which was found to occur to a fetuin concentration of 1 ng/$m\ell$.

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