Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Anti-Inflammation Activity of Actinidia polygama

  • Published : 2003.11.01
Download PDF
⟨ Previous Next ⟩

Abstract

The fruit of Actinidia polygama (AP) has long been used as a folk medicine in Korea for treating pain, rheumatic arthritis and inflammation. The present investigation was carried out to determine the in vivo and in vitro anti-inflammatory activity of AP using several animal models of inflammation. The 70% ethanol extract of the fruit of AP significantly inhibited acetic acidinduced, vascular permeability in a dose dependent manner (23%, 38%, and 41 % inhibition at doses of 200 mg/kg, 500 mg/kg and 1000 mg/kg, respectively). This effect was maintained in AP water-soluble fraction (APW). The APW fraction also showed significant inhibitory activity against the rat paw edema induced by a single treatment of carrageenan. In vitro experiments were performed to demonstrate the inhibitory activities of APW (100 $\mu$ g/ml) on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production. The results showed that APW dose-dependently suppressed LPS-induced NO production in RAW 264.7 macrophages without a notable cytotoxic effect and also decreased inducible NO synthase (iNOS) protein expression. APW also showed a significant inhibitory effect in LPS-induced $PGE_2$ production and cyclooxygenase-2 (COX-2) expression.

Keywords

References

  1. Harris, S. G., Padilla, J., Koumas, L., Ray, D., and Phipps, R. P., Prostaglandins as modulators of immunity. Trends. Immunol., 23(3),144-150 (2002) https://doi.org/10.1016/S1471-4906(01)02154-8
  2. Herschman, H.R., Prostaglandin synthase 2. Biochem. Biophys. Acta., 1299(1), 125-140 (1996) https://doi.org/10.1016/0005-2760(95)00194-8
  3. Kapu, S. D., Ngwai, Y. B., Kayode, O., Akah, P.A.., Wambebe, C., and Gamaniel, K., Anti-inflammatory, analgesic and antilymphocytic activities of the aqueous extract of Crinum giganteum. J. Ethnopharmacol., 78, 7-13 (2001) https://doi.org/10.1016/S0378-8741(01)00308-7
  4. Kim, J. S., Kim, J. M., Jung, H. C., and Song, I. S., Expression of cyclooxygenase-2 in human neutrophils activated by helicobacter pylori water-soluble proteins: possible involvement of NF-kappaB and MAP kinase signaling pathway. Dig. Dis. Sci., 46(10), 2277-2284 (2002) https://doi.org/10.1023/A:1011939704802
  5. Lee, E. B., Li, D.W., Hyun, J. E., Kim, I. H., and Whang, W. K., Anti-inflammatory activity of methanol extract of Kalopanx pictus bark and its fractions. J. Ethnopharmacol., 77, 197-201 (2001) https://doi.org/10.1016/S0378-8741(01)00301-4
  6. Lee, S. H. , Soyoola, E., Chanmugam, P., Hartt, S., Sun, W., Zhong, H., Liou, S., Simmons, D., and Hwang, D., Selective expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide. J. Biol. Chem., 267(36), 25934-25938 (1992)
  7. Olajide, O. A., Awe, S. O., Makinde, J. M., Ekhelar, A. I., Olusola. A., Morebise, O., and Okpako, D. T., Studies on the anti inflammatory, antipyretic and analgesic properties of Alstonia boonei stem bark. J. Ethnopharmacol., 71(1-2), 179-186 (2000) https://doi.org/10.1016/S0378-8741(99)00200-7
  8. Srinivasan, K., Muruganandan, S., Lal, J., Chandra, S., Tandan, S. K., and Prakash, V. R., Evaluation of anti-inflammatory activity of Pongamia pinnata leaves in rats. J. Ethnopharmacol., 78(2-3), 151-157 (2001) https://doi.org/10.1016/S0378-8741(01)00333-6
  9. Vane, J. R. and Botting, R. M., Anti-inflammatory drugs and their mechanism of action. Inflamm Res., 2, S78-87. (1998)