Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Changes of Strength and Stiffness of Freeze-Dried Bovine Cortical Bone according to Rehydration Time in Electrolyte Solution

동결건조한 소의 치밀골에서 전해질용액의 침지시간에 따른 Strength와 Stiffness의 변화

  • 김남수 (전북대학교 수의과대학) ;
  • 장세웅 (전북대학교 수의과대학) ;
  • 김희은 (전북대학교 수의과대학) ;
  • 정인성 (건국대학교 수의과대학) ;
  • 최성진 (전북대학교 수의과대학) ;
  • 최인혁 (전북대학교 수의과대학)
  • Published : 2003.12.01
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Abstract

Transplanted cortical bone grafts of freeze-dried bones also function as sustaining for defected bones, however, it has less strength and is fragile without rehydration. In this study, strength and stiffness of freeze-dried bone from bovine cortical bones were evaluated by three point bending test according to different time frames such as rehydration times of 0.5, 3, 6, 12 and 24 hrs in electrolyte solution and was compared with those of frozen bones. The strength and stiffness of frozen bone were $264.4\pm36.7$ MPa, $17.0\pm1.5$ GPa, respectively. The strength and stiffness of freeze-dried bone which fat was removed by treatments of chloroform-methanol solutions for 6 days, then was freeze-dried at $-80^{\circ}C$ and sterilized with ethylene oxide gas, were $224.9\pm27.6$ MPa, $19.2\pm2.8$ GPa, respectively. The strength and stiffness of feeze-dried bone were decreased 15.0% and increased 13.2% than these of frozen bone, respectively. The strength and stiffness of freeze-dried bone rehydrated for 6 hrs were restored to 96.0% strength and 99.2% stiffness of frozen bone. The rehydration time of freeze-dried bone which had the highest strength and stiffness was six hours and three hours, respectively. The results of the mathematica program for the variation of the strength and stiffness showed 3 hours and 30 minutes of rehydration time in electrolyte solution for the best condition in the strength and stiffness which was adequate to treat freeze-dried cortical bone.

Keywords

References

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