Synthesis and Characterization of a Receptor-Targeting Contrast Agent

  • Yang, Taegyun (Magnetic Resonance Team, Korea Basic Science Institute) ;
  • Park, Ji-Hyung (Magnetic Resonance Team, Korea Basic Science Institute) ;
  • Lee, Seung-Cheol (Magnetic Resonance Team, Korea Basic Science Institute) ;
  • Kim, Cheol-Su (Magnetic Resonance Team, Korea Basic Science Institute) ;
  • Cho, Jee-Hyun (Magnetic Resonance Team, Korea Basic Science Institute) ;
  • Lee, Chul-Hyun (Magnetic Resonance Team, Korea Basic Science Institute) ;
  • Cheong, Chae-Joon (Magnetic Resonance Team, Korea Basic Science Institute)
  • Published : 2003.06.01

Abstract

We synthesized a contrast agent for MRI that is capable of binding to the ABP-1 receptor and enhancing the contrast of the targeted cells. We used a lysine dendrimer (G=3)DTPA[Gd] as the contrast agent and synthesized a biotinylated polyclonal antibody for ABP-1 as the first antibody. Lysine dendrimers were prepared using the solid phase peptide synthesis method.$^3$ Amino-terminated lysine dendrimers were then coupled to DTPA using the anhydride method. Gd was complexed with the DTPA-lysine dendrimer in an acidic solution of 3 eq GdCl$_3$ to one of DTPA. The lysine dendrimer-DTPA[Gd] and avidin were conjugated in MES solution, pH 6.0, using EDC as the coupling reagent. The biotin-avidin system was used to link the polyclonal antibody and contrast agent. K562 cells were used for imaging.

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